Detection of the root-knot nematode Meloidogyne luci Carneiro et al., 2014 (Tylenchida: Meloidogynidae) in vegetable fields of Samsun Province, Turkey

Year 2018, Volume: 42 Issue: 3, 229 - 237, 31.05.2018

Gökhan Aydınlı

https://doi.org/10.16970/entoted.409941

Cited By: 22

Abstract

The root-knot nematode, Meloidogyne
luci Carneiro et al., 2014 (Tylenchida: Meloidogynidae), is commonly found
in greenhouses in Samsun Province in northern Turkey but has not been reported
in open fields. However, the most recent study on the distribution of root-knot
nematodes in open fields of this region was conducted more than 20 years ago
and identification was based on perineal patterns. Therefore, the aim of the
present study was to update the distribution of Meloidogyne spp. in the
vegetable fields of Samsun Province. For that purpose, soil samples were
collected from 50 vegetable fields during July 2017. Nematode isolates obtained
from bioassay tests were identified based on their esterase enzyme phenotype
and identification was confirmed with molecular techniques. Root-knot nematodes
were detected in 20% of the fields sampled. Meloidogyne luci was the
most prevalent species, as was reported
from greenhouses in Samsun Province, followed by Meloidogyne arenaria
(Neal, 1889) Chitwood, 1949 and Meloidogyne incognita (Kofoid
& White, 1919) Chitwood, 1949. Meloidogyne luci is reported for the
first time from open fields in Turkey with this study.

Keywords

Distribution, esterase phenotype, Meloidogyne, mitochondrial DNA, Turkey

Samsun İli sebze alanlarında kök-ur nematodu Meloidogyne luci Carneiro et al., 2014 (Tylenchida: Meloidogynidae)’nin belirlenmesi

Abstract

Türkiye’nin
kuzeyindeki Samsun İli’ndeki seralarda yaygın bulunan kök-ur nematodu Meloidogyne
luci Carneiro et al., 2014 (Tylenchida: Meloidogynidae), bu çalışmadan önce
açık alanlarda tespit edilmemiştir. Ayrıca, bu bölgedeki açık alanlarda kök-ur
nematodlarının dağılımı ile ilgili en son çalışma, 20 yıldan daha uzun süre
önce yürütülmüş ve teşhis perineal desenlere göre yapılmıştır. Bu yüzden, mevcut
çalışmanın amacı, Samsun ilindeki sebze tarlalarında Meloidogyne
türlerinin dağılımına ait sonuçların güncellenmesidir. Bu amaçla, Temmuz
2017’de 50 sebze tarlasından toprak örnekleri alınmıştır. Test bitkilerinden
elde edilen nematod izolatları, esteraz enzim fenotiplerine göre teşhis edilmiş
ve teşhis moleküler teknikler ile teyit edilmiştir. Örnekleme yapılan sebze
tarlalarının %20’sinde kök-ur nematodları tespit edilmiştir. Samsun ilinde daha
önce seralarda belirlenen M. luci, en yaygın tür olarak tespit
edilirken, bu türü Meloidogyne arenaria (Neal, 1889) Chitwood, 1949 ve
Meloidogyne incognita (Kofoid & White,
1919) Chitwood, 1949 takip etmiştir. Türkiye’de açık alanlarda M. luci’ nin varlığı ilk defa bu çalışma
ile bildirilmiştir.

Keywords

Dağılım, esteraz fenotipi, Meloidogyne, mitokondrial DNA, Türkiye

References

• Aydınlı, G. & S. Mennan, 2016. Identification of root-knot nematodes (Meloidogyne spp.) from greenhouses in the Middle Black Sea Region of Turkey. Turkish Journal of Zoology, 40 (5): 675-685.
• Aydınlı, G., S. Mennan, Z. Devran, S. Sirca & G. Urek, 2013. First report of the root-knot nematode Meloidogyne ethiopica on tomato and cucumber in Turkey. Plant Disease, 97 (9): 1262.
• Bora, A., 1970. Karadeniz bölgesi bitki parazit nematodlarının tür ve yayılış alanlarının tesbiti ve ilaçlı mücadele imkanları üzerinde araştırmalar. Bitki Koruma Bülteni, 10 (1): 53-71.
• Brito, J. A., R. Kaur, R. Cetintas, J. D. Stanley, M. L. Mendes, E. J. McAvoy, T. O. Powers & D. W. Dickson, 2008. Identification and isozyme characterization of Meloidogyne spp. infecting horticultural and agronomic crops, and weed plants in Florida. Nematology, 10 (5): 757-766.
• Carneiro, R. M. D. G., V. R. Correa, M. R. A. Almeida, A. C. M. M. Gomes, A. M. Deimi, P. Castagnone-Sereno & G. Karssen, 2014. Meloidogyne luci n. sp. (Nematoda: Meloidogynidae), a root-knot nematode parasitising different crops in Brazil, Chile and Iran. Nematology, 16 (3): 289-301.
• Conceicão, I. L., E. A. Tzortzakakis, P. Gomes, I. Abrantes & M. J. Cunha, 2012. Detection of the root-knot nematode Meloidogyne ethiopica in Greece. European Journal of Plant Pathology, 134: 451-457.
• Esteves, I., C. Maleita & I. Abrantes, 2015. Root-lesion and root-knot nematodes parasitizing potato. European Journal of Plant Pathology, 141: 397-406.
• Garcia, L. E. & M. V. Sanchez-Puerta, 2012. Characterization of a root-knot nematode population of Meloidogyne arenaria from Tupungato (Mendoza, Argentina). Journal of Nematology, 44 (3): 291-301.
• Gerič Stare, B., P. Strajnar, N. Susic, G. Urek & S. Širca, 2017. Reported populations of Meloidogyne ethiopica in Europe identified as Meloidogyne luci. Plant Disease, 101 (9): 1627-1632.
• Hall, T. A., 1999. Bioedit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/nt. Nucleic Acids Symposium Series, 41: 95-98.
• Janssen, T., G. Karssen, M. Verhaeven, D. Coyne & W. Bert, 2016. Mitochondrial coding genome analysis of tropical root-knot nematodes (Meloidogyne) supports haplotype-based diagnostics and reveals evidence of recent reticulate evolution. Scientific Reports, 6: 22591.
• Karuri, H. W., D. Olago, R. Neilson, E. Mararo & J. Villinger, 2017. A survey of root knot nematodes and resistance to Meloidogyne incognita in sweet potato varieties from Kenyan fields. Crop Protection, 92: 114-121.
• Kaşkavalcı, G. & C. Öncüer, 1999. Investigations on distribution and economic importance of Meloidogyne Goeldi, 1887 (Tylenchida: Meloidogynidae) species found in the major areas of hot climate vegetables in Aydın Province. Turkish Journal of Entomology, 23 (2): 149-160.
• Katı, T. & S. Mennan, 2006. “Researches on species and race determination of root-knot nematodes (Meloidogyne spp.) found in greenhouse of Samsun, Turkey, 130”. Abstracts of the 28th International Symposium of the European Society of Nematologists (5-9 June 2006, Blagoevgrad, Bulgaria), 130 pp.
• Kiewnick, S., M. Holterman, S. Van Den Elsen, H. Van Megen, J. E. Frey & J. Helder, 2014. Comparison of two short DNA barcoding loci (COI and COII) and two longer ribosomal DNA genes (SSU & LSU rRNA) for specimen identification among quarantine root-knot nematodes (Meloidogyne spp.) and their close relatives. European Journal of Plant Pathology, 140: 97-110.
• Maleita, C. M., M. J. Simões, C. Egas, R. H. C. Curtis & I. M. De O. Abrantes, 2012. Biometrical, biochemical, and molecular diagnosis of Portuguese Meloidogyne hispanica isolates. Plant Disease, 96 (6): 865-874.
• Maleita, C., I. Esteves, J. M. S. Cardoso, M. J. Cunha, R. M. D. G. Carneiro & I. Abrantes, 2018. Meloidogyne luci, a new root-knot nematode parasitizing potato in Portugal. Plant Pathology, 67 (2): 366-376.
• Mennan, S. & O. Ecevit, 1996. “Studies on biology, distribution and the ratio of infestation of root-knot nematodes (Meloidogyne spp.) in summer vegetable growing area in Bafra and Carşamba Plains, 700-705”. Proceedings of the 3rd Turkish National Congress of Entomology (24–28 September 1996, Ankara, Turkey), 716 pp.
• Moens, M., R. N. Perry & J. L. Starr, 2009. “Meloidogyne Species a Diverse Group of Novel and Important Plant Parasites,1-17”. In: Root- Knot Nematodes (Eds. R. N. Perry, M. Moens & J. L. Starr). CABI, London, 488 pp.
• Onkendi, E.M., G. M. Kariuki, M. Marais & L. N. Moleleki, 2014. The threat of root-knot nematodes (Meloidogyne spp.) in Africa: A review. Plant Pathology, 63 (4): 727-737.
• Randig, O., M. Bongiovanni, R. M. D. G. Carneiro & P. Castagnone-Sereno, 2002. Genetic diversity of root-knot nematodes from Brazil and development of SCAR markers specific for the coffee-damaging species. Genome, 45: 862-870.
• Sirca, S., G. Urek & G. Karssen, 2004. First report of the root-knot nematode Meloidogyne ethiopica on tomato in Slovenia. Plant Disease, 88: 680.
• Strajnar, P., S. Sirca, M. Knapic & G. Urek, 2011. Effect of Slovenian climatic conditions on the development and survival of the root-knot nematode Meloidogyne ethiopica. European Journal of Plant Pathology, 129: 81-88.
• Tesarova, B., M. Zouhar & P. Ryšanek, 2003. Development of PCR for specific determination of root-knot nematode Meloidogyne incognita. Plant Protection Science, 39 (1): 23-28.
• TUIK, 2017. Turkish Statistical Institute, Ankara. Crop Production Statistics. (Web page: https://biruni.tuik.gov.tr/medas/?kn=92&locale=en) (Date accessed: 5 March 2018).
• Van Gundy, S. D., 1985. “Ecology of Meloidogyne spp. - Emphasis on Environmental Factors Affecting Survival and Pathogenicity, 177-182”. In: An Advance Treatise on Meloidogyne. Vol. I, Biology and Control (Ed: J. N. Sasser & C. C. Carter) Raleigh, NC, USA: North Carolina State University Graphics, 223 pp.
• Wesemael, W. M. L., N. Viaene & M. Moens, 2011. Root-knot nematodes (Meloidogyne spp.) in Europe. Nematology, 13 (1): 3-16.
• Wishart, J., M. S., Phillips & V. C. Blok, 2002. Ribosomal intergenic spacer: A polymerase chain reaction diagnostic for Meloidogyne chitwoodi, M. fallax, and M. hapla. Phytopathology, 92: 884-892.
• Yüksel, H., 1974. Kök-ur nematodlarının (Meloidogyne spp.) Türkiyedeki durumu ve bunların populasyon problemleri üzerine düşünceler. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 5 (1): 83-105.
• Zijlstra, C., D. T. H. M. Donkers-Venne & M. Fargette, 2000. Identification of Meloidogyne incognita, M. javanica and M. arenaria using sequence characterised amplified region (SCAR) based PCR assays. Nematology, 2(8): 847-853.