Detection of the root-knot nematode Meloidogyne luci Carneiro et al., 2014 (Tylenchida: Meloidogynidae) in vegetable fields of Samsun Province, Turkey

Year 2018, Volume: 42 Issue: 3, 229 - 237, 31.05.2018

Gökhan Aydınlı

https://doi.org/10.16970/entoted.409941

Cited By: 23

Abstract

The root-knot nematode, Meloidogyne
luci Carneiro et al., 2014 (Tylenchida: Meloidogynidae), is commonly found
in greenhouses in Samsun Province in northern Turkey but has not been reported
in open fields. However, the most recent study on the distribution of root-knot
nematodes in open fields of this region was conducted more than 20 years ago
and identification was based on perineal patterns. Therefore, the aim of the
present study was to update the distribution of Meloidogyne spp. in the
vegetable fields of Samsun Province. For that purpose, soil samples were
collected from 50 vegetable fields during July 2017. Nematode isolates obtained
from bioassay tests were identified based on their esterase enzyme phenotype
and identification was confirmed with molecular techniques. Root-knot nematodes
were detected in 20% of the fields sampled. Meloidogyne luci was the
most prevalent species, as was reported
from greenhouses in Samsun Province, followed by Meloidogyne arenaria
(Neal, 1889) Chitwood, 1949 and Meloidogyne incognita (Kofoid
& White, 1919) Chitwood, 1949. Meloidogyne luci is reported for the
first time from open fields in Turkey with this study.

Keywords

Distribution, esterase phenotype, Meloidogyne, mitochondrial DNA, Turkey

Samsun İli sebze alanlarında kök-ur nematodu Meloidogyne luci Carneiro et al., 2014 (Tylenchida: Meloidogynidae)’nin belirlenmesi

Abstract

Türkiye’nin
kuzeyindeki Samsun İli’ndeki seralarda yaygın bulunan kök-ur nematodu Meloidogyne
luci Carneiro et al., 2014 (Tylenchida: Meloidogynidae), bu çalışmadan önce
açık alanlarda tespit edilmemiştir. Ayrıca, bu bölgedeki açık alanlarda kök-ur
nematodlarının dağılımı ile ilgili en son çalışma, 20 yıldan daha uzun süre
önce yürütülmüş ve teşhis perineal desenlere göre yapılmıştır. Bu yüzden, mevcut
çalışmanın amacı, Samsun ilindeki sebze tarlalarında Meloidogyne
türlerinin dağılımına ait sonuçların güncellenmesidir. Bu amaçla, Temmuz
2017’de 50 sebze tarlasından toprak örnekleri alınmıştır. Test bitkilerinden
elde edilen nematod izolatları, esteraz enzim fenotiplerine göre teşhis edilmiş
ve teşhis moleküler teknikler ile teyit edilmiştir. Örnekleme yapılan sebze
tarlalarının %20’sinde kök-ur nematodları tespit edilmiştir. Samsun ilinde daha
önce seralarda belirlenen M. luci, en yaygın tür olarak tespit
edilirken, bu türü Meloidogyne arenaria (Neal, 1889) Chitwood, 1949 ve
Meloidogyne incognita (Kofoid & White,
1919) Chitwood, 1949 takip etmiştir. Türkiye’de açık alanlarda M. luci’ nin varlığı ilk defa bu çalışma
ile bildirilmiştir.

Keywords

Dağılım, esteraz fenotipi, Meloidogyne, mitokondrial DNA, Türkiye

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