"Mor rengin farklı tonlarında": ticari konsantrasyondaki farklı siyah chokeberry meyve özütü konsantrasyonlarının [Aronia melanocarpa (Michx) Elliott] meyve sineği Drosophila melanogaster Meigen 1830’in hareket özelliği bileşenleri ve kanat morfolojisi üzerindeki etkileri

Year 2019, Volume: 43 Issue: 1, 3 - 16, 01.03.2019

Tatjana Savić , Gordana Radivojević Jelena Trajković Nataša Bajalović Luka Lučić Dragana Miličić Vladimir Tomić Slobodan Makarov Sonja Duletić-laušević Sofija Pavković-lučić

https://doi.org/10.16970/entoted.480766

Abstract

Son yıllarda, meyve ve sebzelerin içindeki biyoaktif bileşiklerin oksidatif stresi düşürdüğü, böylece birçok insan hastalığının riskini azaltan faydalı bir etkiye sahip olduğu yaygın olarak kabul edilmektedir. Mevcut in vivoçalışmanın amacıDrosophila melanogaster Meigen, 1830 larvası kullanılarak Aronia melanocarpa (Michx) Elliott’nın meyve özütünün muhtemel koruyucu etkilerini değerlendirmektir. Çalışma 2016 yılında, Belgrad Üniversitesi, Biyolojik Araştırma Enstitüsü'ndeki, Popülasyon Genetiği ve Ekogenotoksikoloji Bölümü’nde gerçekleştirilmiştir. Uygulamalar ile aynı anda, metil metansülfonat (MMS) ile karıştırılmış aynı konsantrasyondaki siyah chokeberry meyve özütüyle yan uygulamalar yapılmıştır. Hareket özelliği ve morfolojik karakterler izlenmiştir. Konsantre meyve özütünde, MMS + Konsantre meyve özütünde ve yalnızca MMS’de gelişen sinekler, uzun gelişme süresi, daha düşük yaşama gücü sergilemiş ve kanat gelişimi de olumsuz etkilenmiştir. Olumlu biyolojik etkiler %2 ile 25 arası A. melanocarpaözütlü substratlarda gelişen sineklerde gözlenmiştir. Sadece MMS + %25 meyve özütü karışımı, diğer MMS yan uygulamaları ile karşılaştırıldığında hareket bileşenleri ve kanat gelişiminin her ikisinde de tercih edilebilir etkiler göstermiş, bu karışımın MMS tarafından verilen hasara karşı hücreyi koruduğuna işaret etmiştir.

Keywords

gelişme süresi, yumurtadan çıkış dinamiği, metil metansülfonat, yaşama gücü, kanat şekli, kanat boyutu

"In different shades of purple": effects of different concentrations of commercial black chokeberry fruit extract [Aronia melanocarpa (Michx) Elliott] on fitness components and wing morphology of the fruit fly, Drosophila melanogaster Meigen, 1830

Abstract

It is now widely accepted that bioactive compounds of fruits and vegetables reduce oxidative stress, thus having the beneficial effect of decreasing the risk of many human diseases. The aim of this in vivostudy was to evaluate the possible protective effects of Aronia melanocarpa(Michx) Elliott fruit extract using Drosophila melanogasterMeigen, 1830 larvae. Study was done in the year 2016, in Department of Genetics of Populations and Ecogenotoxicology at the Institute for Biological Research, University of Belgrade. Simultaneously with treatments, co-treatments with the same concentrations of black chokeberry fruit extract mixed with a methyl methanesulfonate (MMS) were performed. Fitness traits and morphological characters were monitored.Flies fed on undiluted fruit extract, on MMS + undiluted fruit extract and on MMS, exhibited a prolonged developmental time, lower viability and negatively-impacted wing development. Positive biological effects were observed in flies that developed on substrates with 2 and 25% A. melanocarpaextract. Only a mixture of MMS + 25% fruit extract showed positive effects on both fitness components and wing development in comparisonwith other MMS co-treatments, indicating the ability of this concentration to protect the cells from MMS-induced damage.

Keywords

developmental time, dynamic of eclosion, methyl methanesulfonate, viability, wing shape, wing size

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