Efficacy of entomopathogenic nematodes against neonate larvae of Capnodis tenebrionis (L., 1758) (Coleoptera: Buprestidae)

Year 2019, Volume: 43 Issue: 3, 279 - 285, 01.09.2019

Çiğdem Şahin Uğur Gözel

https://doi.org/10.16970/entoted.543469

Cited By: 3

Abstract

Entomopathogenic nematodes (EPN) have a high potential for control of pests living in isolated places such as underground or galleries.In this study, mortality rates of Capnodis tenebrionis (L., 1758) (Coleoptera: Buprestidae) larvae from four EPN species Steinernema affine Bovien, 1937, Steinernema carpocapsae Weiser, 1934, Steinernema feltiae Filipjev, 1934 (Rhabditida: Steinernematidae) and Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) collected from Turkey under controlled conditions were determined. EPN used in the study were cultured on Galleria mellonella (L., 1758) (Lepidoptera: Pyralidae). Adults of C. tenebrionis were collected from the orchards of Çanakkale Province and, eggs and larvae were cultured under controlled conditions. Three densities of EPN species, viz. 50, 500 and 1000 infective juveniles/C. tenebrionis, were applied in 12-well plates. Cherry saplings were planted into pots with sterilized soil mixture and 10 neonate larvae of C. tenebrionisadded to each pot. To each pot, 40,000 infective juveniles were applied for each EPN species in 10 ml of water. Mortalities of C. tenebrionis larvae were determined 1, 3, 5 and 7 d after application. In the plates, mortality of C. tenebrionis larvae increased with time after EPN application. For all application rates, mortality of C. tenebrionis larvae was 100% by day 5. Mortality of C. tenebrionis larvae ranged between 50 and 90% depending on species and time in pots. Efficacy studies were conducted in 2016 in Çanakkale. Research on the efficacy of EPN species that have a high mortality under controlled conditions is important to determine their potential to control the target pest.

Keywords

Capnodis tenebrionis, Heterorhabditis bacteriophora, Steinernema affine, Steinernema carpocapsae, Steinernema feltiae

Supporting Institution

Scientific and Technological Research Council of Turkey (TUBITAK TOVAG project 116O361).

Project Number

Scientific and Technological Research Council of Turkey (TUBITAK TOVAG project 116O361).

Thanks

This research was a part of the doctoral thesis of first author and it was financially supported by the Scientific and Technological Research Council of Turkey (TUBITAK TOVAG project 116O361).

Capnodis tenebrionis (L., 1758) (Coleoptera: Buprestidae)’in ilk dönem larvalarına karşı entomopatojen nematodların etkinliği

Abstract

Toprak altı ve galeriler gibi izole alanlarda yaşayan zararlıların mücadelesinde entomopatojen nematodlar (EPN) yüksek bir potansiyele sahiptir.Bu çalışmada Türkiye’den elde edilen dört entomopatojen nematod (EPN) türünün Steinernema affine Bovien, 1937, Steinernema carpocapsae Weiser, 1955, Steinernema feltiae Filipjev, 1934 (Rhabditida: Steinernematidae) ve Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) kontrollü koşullarda Capnodis tenebrionis (L., 1758) (Coleoptera: Buprestidae) larvalarında meydana getirdikleri ölüm oranları belirlenmiştir. Çalışmada kullanılan EPN’ler laboratuvarda Galleria mellonella (L., 1758) (Lepidoptera: Pyralidae) üzerinde üretilmiştir. Capnodis tenebrionis erginleri Çanakkale ili meyve bahçelerinden toplanmış ve kontrollü koşullarda yumurta ve larvaları üretilmiştir. EPN türlerinin 50, 500 ve 1000 infektif jüvenil/C. tenebrionis olmak üzere 3 farklı yoğunluğu 12 hücreli kuyucuklarda uygulanmıştır. Saksı denemelerinde, sterilize edilmiş toprak karışım içeren saksılara kiraz fidanları dikilmiş ve her saksıya 10’ar adet 1. dönem C. tenebrionis larvası bulaştırılmıştır. Kiraz fidanları sterilize toprak karışımı içeren saksılara dikilmiş ve her saksıya 10 adet 1. dönem C. tenebrionis larvası aktarılmıştır. Her saksıya her bir EPN türü için 40.000 infektif juvenile 10 ml su içerisinde uygulanmıştır. C. tenebrionis larvalarının ölüm oranları uygulamadan 1, 3, 5 ve 7 gün sonra belirlenmiştir.  Tüm uygulama oranlarında C. tenebrionis larvalarının ölüm oranı 5. günde %100’dür. Capnodis tenebrionis larvalarının ölüm oranları türe ve zamana bağlı olarak %50-90 arasında değişiklik göstermiştir. Etkinlik çalışmaları 2016 yılında Çanakkale ilinde gerçekleştirilmiştir. Kontrollü koşullar altında yüksek ölüm oranına sahip olan EPN türlerinin etkinliklerinin araştırılması hedef zararlının kontrolü için potansiyellerini belirlemek için önemlidir.

Keywords

Capnodis tenebrionis, Heterorhabditis bacteriophora, Steinernema affine, Steinernema carpocapsae, Steinernema feltiae

Project Number

Scientific and Technological Research Council of Turkey (TUBITAK TOVAG project 116O361).

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