Türkiye’nin Adana ve Mersin illerinden toplanan Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae) popülasyonlarıyla ilişkili kültüre alınabilen bakteriler ve entomopatojen özelliklerinin belirlenmesi

Year 2020, Volume: 44 Issue: 1, 13 - 22, 01.03.2020

Şebnem Tireng Karut , Kamil Karut , Yeşim Aysan

https://doi.org/10.16970/entoted.578542

Cited By: 4

Abstract

Bünyesinde çeşitli bakterileri barındıran, Beyazsinek, Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae) tarımsal üretimlerde ekonomik kayıplara neden olan en önemli zararlı böcek türlerinden birisidir. Bu çalışmada B. tabaci’nin bakteriyel florasını belirlemek ve bu bakterilerin etkinliğini ortaya çıkarmak amacıyla 2014 yılında iki farklı ilden (Mersin ve Adana) sırasıyla susam ve kavun bitkilerinden örnekler toplanmıştır. Morfolojik, MALDI-TOF MS ve moleküler tanı yöntemleri kullanılarak yapılan analizler sonucunda toplam 9 adet bakteri türü saptanmıştır. Susam tarlasından toplanan örneklerden Bacillus sp., Methylobacterium sp., Microbacterium sp., Serratia marcescens, Sphingomonas sp.; kavun bitkisinden toplanan örneklerden Acinetobacter lwoffii, Bacillus cereus, Staphylococcus hominis ve Staphylococcus warneri türleri belirlenmiştir. Bemisia tabaci erginlerine (B biyotip) karşı yapılan biyolojik etkinlik çalışmalarında beyazsinekler, kontrol olarak böcek besini (sakkaroz+su), ilaçlı kontrol (acetamiprid) ve bakteri süspansiyonlarıyla beslenmişlerdir. İlk defa bu çalışma kapsamında B. tabaci’den izole edilen ve entomopatojen bir bakteri olan S. marcescens ergin beyazsineğe karşı %72 oranında başarılı olmuştur. Diğer izolatların etki oranları %25’in altında kalmıştır. Serratia marcescens ile elde edilen bu başarı, izolatın zararlı böcekler üzerindeki patolojisi ile ilgili çalışmaların devam ettirilmesi gerektiği sonucunu ortaya çıkarmıştır.

Keywords

Bemisia tabaci, biyolojik mücadele, entomopatojen bakteri, Serratia marcescens, simbiyont

Project Number

FDK-2015-5239 and TAGEM-BS-13/09-01/02-05

Culturable bacterial strains isolated from Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae) populations of Adana and Mersin Provinces of Turkey and their entomopathogen characteristics

Abstract

Whitefly, Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae) hosting various bacteria is among the most significant insect pest causing economic losses in agricultural production. A study was conducted to determine the bacterial flora of B. tabaci and to identify the efficiency of these bacteria against B. tabaci. Samples were collected from sesame and melon plants in Mersin and Adana Provinces of Turkey in 2014. Nine bacterial strains were identified by morphological, MALDI-TOF MS and molecular identification methods to species level. Bacillus sp., Methylobacterium sp., Microbacterium sp., Serratia marcescens and Sphingomonas sp. were identified from the samples collected from sesame and Acinetobacter lwoffii, Bacillus cereus, Staphylococcus hominis and Staphylococcus warneri were identified from the samples collected from melon. To determine biological efficiency against B. tabaci adult (biotype B), whiteflies were fed with insect food (control, sucrose + water), insecticidal control (acetamiprid) and bacterial suspensions. The entomopathogenic bacteria S. marcescens isolated from B. tabaci for the first time in this study yielded an efficiency of 72% against adult whitefly. However, the other strains had efficiencies below 25%. It was concluded, given the efficiency of S. marcescens, that further research should be conducted on the pathology of entomopathogenic bacteria in pest insects.

Keywords

Bemisia tabaci, biological control, entomopathogen bacteria, Serratia marcescens, symbiont

Supporting Institution

Çukurova University Scientific Research Projects Department and General Directorate of Agricultural Researches and Policies.

Project Number

FDK-2015-5239 and TAGEM-BS-13/09-01/02-05

Thanks

This study presents partial results of Ph.D. project supported by Çukurova University Scientific Research Projects Department (FDK-2015-5239) and General Directorate of Agricultural Researches and Policies (TAGEM-BS-13/09-01/02-05). Acknowledgements are extended to Assoc. Prof. Dr. Zeki GÖKALP (a certified English translator and expert in Biosystems Engineering) for his technical supports for the presentation of this paper.

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