Histopathological changes of some resistant and susceptible wheat cultivars caused by plant parasitic nematodes

Year 2020, Volume: 44 Issue: 1, 91 - 100, 01.03.2020

Ece Börteçine Kasapoğlu Uludamar Şenay Karabıyık İbrahim Halil Elekcıoglu

https://doi.org/10.16970/entoted.621047

Abstract

Root lesion nematodes, Pratylenchus thornei Sher & Allen, 1953 and Pratylenchus neglectus (Rensch, 1924) (Tylenchida: Pratylenchidae), and cereal cyst nematode, Heterodera avenae Wollenweber, 1924 (Tylenchida: Heteroderidae) are economically important migratory and sedentary endoparasites on host plant roots. In this study, cellular variations in roots of resistant and susceptible wheat cultivars infected by P. thornei, P. neglectus and H. avenae were investigated between 2017 and 2018. Wheat cultivars were infected with P. neglectus, P. thornei, and H. avenae, separately and H. avenae and P. thornei together. Twelve weeks after inoculation, roots were washed, embedded in paraffin, cut by microtome. The root cell slides were examined under light microscope for histopathological changes in the root cells. The root lesion nematodes moved from epidermal to cortical cells by damaging the cells along the feeding path. Due to the nematode infection, cortical cells of most wheat cultivars were destroyed by the development of many cavities in the root tissue. Also, it was observed that many root lesion nematodes fed collectively on the same site either stretched out or coiled in different cell layers. Heterodera avenae fed on the cortex cells as did root lesion nematode.

Keywords

Cereal cyst nematodes, Heterodera avenae, histopathology, root lesion nematodes, wheat

Supporting Institution

Tübitak

Project Number

214O419

Thanks

The research for this paper was financially supported by the Scientific and Technological Research Council of Turkey (TUBITAK Project no. 2140419). In developing the ideas presented here, we thank Prof. Dr. Sinan Eti (Çukurova University, Agriculture Faculty, Department of Horticulture) for helpful input.

Bitki paraziti nematodlarının neden olduğu bazı dayanıklı ve hassas buğday çeşitlerinin histopatolojik değişiklikleri

Abstract

Kök lezyon nematodları, Pratylenchus thornei Sher & Allen, 1953 ve Pratylenchus neglectus (Rensch, 1924) (Tylenchida: Pratylenchidae) ve Tahıl kist nematodları Heterodera avenae Wollenweber, 1924 (Tylenchida: Heteroderidae) konukçu bitki köklerinde beslenen ve ekonomik olarak önemli hareketli ve hareketsiz endoparazitlerdir. Bu çalışmada, P. thornei, P. neglectus ve H. avenae ile bulaştırma yapılan dayanıklı ve hassas buğday çeşitlerindeki hücresel değişimler 2017-2018 yılları arasında incelenmiştir. Buğday çeşitleri, P. neglectus, P. thornei ve H. avenae türleri ile ayrı ayrı olarak ve H. avenae ile P. thornei birlikte inokule edilmiştir. İnokulasyondan on iki hafta sonra, buğday çeşitlerinin kökleri yıkanıp parafin içerisine sabitlenmiş, mikrotom ile kesilip kök hücre preparatları hazırlanmış ve kök hücrelerindeki histopatolojik değişimlerin ışık mikroskobu altında incelenmiştir. Kök lezyon nematodlarının, epidermal hücrelerden kortikal hücrelere doğru beslenme yolu boyunca hücrelere zarar vererek hareket ettiği gözlenmiştir. Nematod infeksiyonundan dolayı çoğu buğday çeşidinin, korteks hücrelerinde birçok oyuğun oluşumuyla kök dokusu tahrip olmuştur. Ayrıca, farklı hücrelerde düz ya da kıvrılmış şekilde, aynı bölgede toplu bir şekilde beslenen birçok kök lezyon nematodları gözlenmiştir.Heterodera avenae bireyleri de kök lezyon nematodları gibi korteks hücrelerinden beslenmiştir.

Keywords

Tahıl kist nematodları, Heterodera avenae, histopatoloji, kök lezyon nematodları, buğday

Project Number

214O419

References

• Aydınlı, G., F. Şen & S. Mennan, 2019. Potential of some plant extracts for the control of root-knot nematode Meloidogyne arenaria (Neal, 1889) Chitwood, 1949 (Tylenchida: Meloidogynidae). Kahramanmaraş Sütçü İmam University Journal of Agriculture Nature, 22 (3): 414-420.
• Castillo, P., N. Vovlas & R. M. Jiménez-Díaz, 1998. Pathogenicity and histopathology of Pratylenchus thornei populations on selected chickpea varieties. Plant Pathology, 47 (3): 370-376.
• Dababat, A., Ş. Yıldız, V. Çiftçi, N. Duman & M. İmren, 2019. Occurrence and seasonal variation of the root lesion nematode Pratylenchus neglectus on cereals in Bolu, Turkey. Turkish Journal of Agriculture and Forestry, 43 (1): 21-27.
• Duncan, L. & M. Moens, 2006. “Migratory endoparasitic nematodes, 123-152”. In: Plant Nematology (Eds. R. N. Perry & M. Moens). CABI, UK, 542 pp.
• Elekcioğlu, I. H., 1992. Untersuchungen zum Auftreten and zur Verbreitung Phytoparasitaerer Nematoden in den Landwirtschaftlichen Hauptkulturen des Ostmediterranen Gebietes der Türkei. PLITS, (Unpublished) PhD Thesis, Hohenheim, Germany, 120 pp.
• Eti, S., 1987. Über das Pollenschlauchwachstum und die Entwicklung der Samenlagen in Beziehung zum Fruchtansatz und zur Fruchtqualitaetbei der Manderinensorte “Clementine” (Citrus reticulata Blanco). Hohenheim University (Unpublished) PhD Thesis, Hohenheim, Germany, 127 pp.
• FAO, 2019a. Food Outlook - Biannual Report on Global Food Markets. (Web page: www.fao.org/3/ca4526en/ca4526en.pdf) (Date accessed: September 2019).
• FAO, 2019b. GIEWS - Global Information and Early Warning System (Web page: http://www.fao.org/giews/countrybrief/ country.jsp?code=TUR) (Date accessed: September 2019).
• Fard, H. K., E. Pourjam, Z. Tanha Maafi & N. Safei, 2018. Assessment of yield loss of wheat cultivars caused by Heterodera filipjevi under field conditions. Journal of Phytopathology, 116 (5): 299-304.
• Farsi, M., 1996. Genetic variation for tolerance and resistance to Pratylenchus neglectus. Adelaide University, (Unpublished) PhD Thesis, Adelaide, Australia, 380 pp.
• Gheysen, G. & M. G. Mitchum, 2011. How nematodes manipulate plant development pathways for infection. Plant Biology, 14 (4): 415-421.
• Göze Özdemir, F., E. Arıcı, B. Yaşar & İ. H. Elekcioğlu, 2018. Effect of Fusarium culmorum spore suspension on mortality of root lesion nematodes in vitro conditions. Black Sea Journal of Agriculture, 1 (2): 29-33.
• Grymaszewska, G. & W. Golinowski, 1991. Structure of syncytia induced by Heterodera avenae Woll. in roots of susceptible and resistant wheat {Triticum aestivum L.). Journal of Phytopathology, 133: 307-319.
• Holtmann, B., M. Kleine & F. Grundler, 2000. Ultra-structure and anatomy of nematode-induced syncytia in roots of susceptible and resistant sugar beet. Protoplasma, 211 (1-2): 39-50.
• Hooper, D. J., 1986. “Extraction of Free-Living Stages from Soil, 5-30”. In: Laboratory Methods for Work with Plant and Soil Nematodes (Ed. J. F. Southey). Ministry of Agriculture, Fisheries, and Food Technology, Reference Book 402, Her Majesty’s Stationary Office, London, UK, 629 pp.
• İmren, M., 2013. Determination of Cereal Cyst Nematodes (Heterodera avenae group.) on Cereal Fields in Eastern Mediterranean and Southeast Anatolian Regions and Investigation of Some Wheat Genotypes Resistance against to Heterodera avenae (Wollenweber, 1924). Çukurova University, (Unpublished) PhD Thesis, Adana, 105 pp.
• İmren, M., V. Çiftçi, Ş. Yıldız, H. Kütük & A. Dababat, 2017. Occurrence and population dynamics of the root lesion nematode Pratylenchus thornei (Sher and Allen) on wheat in Bolu, Turkey. Turkish Journal of Agriculture and Forestry, 41: 35-41.
• İmren, M., İ. H. Elekcioğlu & H. Özkan, 2015. Determining the resistance of some bread wheat varieties against root lesion nematodes: Pratylenchus thornei and Pratylenchus neglectus (Tylenchida: Pratylenchidae). Journal of Agricultural Sciences, 21 (1): 61-70.
• İmren, M., E. B. Kasapoğlu, A. Dababat, H. Toktay & İ. H. Elekcioğlu, 2014. Investigation the efficiency of resistance gene, Cre 1, against Mediterranean cereal cyst nematode: Heterodera latipons Franklin (Tylenchida: Heteroderidae). Journal of Agricultural Sciences, 20: 261-268.
• Karabıyık, Ş., M. A. Gündeşli, S. Eti, S. Kafkas, M. Güney, M. Zarifikhosroshahi & N. E. Kafkas, 2018. Detection of bud abscission of pistachio via histological analysis. Acta Horticulturae, 1219 (31): 193-198.
• Kasapoğlu, E. B., M. İmren, T. Behmand & İ. H. Elekcioğlu, 2016. “Molecular characterization of cereal cyst nematode; Heterodera avenae and H. latipons diagnostic markers Cre1 and Cre3 in some winter and summer wheat germplasm, 254". Turkey 6. Plant Protection Congress, (5-8 September 2016, Konya, Turkey), 938 pp.
• Kasapoğlu Uludamar, E., 2018. Investigation of Resistance Mechanisms of Root Lesion Nematodes (Pratylenchus thornei and Pratylenhcus neglectus) and Cyst Nematodes (Heterodera avenae and Heterodera latipons) in Wheat. Çukurova University, (Unpublished) PhD thesis, Adana, Turkey, 95 pp.
• Kepenekçi, İ., D. Erdoğuş & P. Erdoğan, 2016. Effects of some plant extracts on root-knot nematodes in vitro and in vivo conditions. Turkish Journal of Entomology, 40 (1): 3-14.
• Kim, Y. H., S. H. Kim & R. D. Riggs, 2010. Differential subcellular responses in resistance soybeans infected with soybean cyst nematode races. Plant Pathology Journal, 26 (2): 154-158.
• Kim, Y. H., R. D. Riggs & S. K. Kim, 1987. Structural changes associated with resistance of soybean to Heterodera glycines. Journal of Nematology, 19 (2): 177-187.
• Linsell, K. J., I. T. Riley, K. A. Davies & K. H. Oldach, 2014. Characterization of resistance to Pratylenchus thornei (Nematoda) in wheat (Triticum aestivum): attraction, penetration, motility, and reproduction. Phytopathology, 104 (2):174-187.
• Mennan, S. & Z. A. Handoo, 2006. Plant-parasitic nematodes associated with cabbages (Brassica oleracea) in Samsun (middle Black Sea Region), Turkey. Nematropica, 36 (1): 99-104.
• Moody, E. H., B. F. Lownsbery & J. H., Ahmed, 1973. Culture of root lesion nematode Pratylenchus vulnus on carrot discs. Journal of Nematology, 5 (3): 255-226.
• Oyekan, P. O., C. D. Blake & J. E. Mitchell, 1972. Histopathology of pea roots axenically infected by Pratylenchus penetrans. Journal of Nematology, 4 (1): 33-35.
• Özdemir, E. & U. Gözel, 2018. Nematicidial activities of essential oils against Meloidogyne incognita on tomato plant. Fresenius Enviromental Bulletin, 27 (6): 4511-4517.
• Pariyar, S., A. Dababat, S. Siddique, G. Erginbaş-Orakçı, A. Elashry, A. Morgounov & F. M. Grundler, 2016. Identification and characterisation of resistance to the cereal cyst nematode Heterodera filipjevi in winter wheat. Nematology, 18 (4): 377-402.
• Seah, S., C. Miller, K. Sivasithamparam & E. S. Lagudah, 2000. Root responses to cereal cyst nematode (Heterodera avenae) in hosts with different resistance genes. New Phytologist, 146 (3): 527-533.
• Toktay, H., 2008. Investigation of Resistance of Some Spring Wheat against to Pratylenchus thornei Sher et Allen (Tylenchida: Pratylenchidae). Çukurova University, (unpublished) PhD Thesis, Adana, Turkey, 117 pp.
• Toktay, H., M. İmren, I. H. Elekcioglu & A. Dababat, 2015. International emmer wheat found in Turkey (Triticum dicoccoides Koern.) and root lesion nematodes of wheat cultivars (Pratylenchus thornei and P. neglectus) determination of their resistance. Turkish Journal of Entomology, 39 (3): 219-227.
• Townshend, J. L. & L. Stobbs, 1981. Histopathology and histochemistry of lesions caused by Pratylenchus penetrans in roots of forage legumes. Canadian Journal of Plant Pathology, 3 (3): 123-186.
• Vovlas, N. & A. Troccoli, 1990. Histopathology of broad bean roots infected by the lesion nematode Pratylenchus penetrans. Nematologia Mediterranea, 18 (2): 239-242.
• Williams, K. J. & J. M. Fisher, 1993. Development of Heterodera avenae Woll. and host cellular responses in susceptible and resistant wheat. Fundamentals of Applied Nematology, 16 (5): 417-423.