Neonicotinoid resistance in adults and nymphs of Bemisia tabaci (Genn., 1889) (Hemiptera: Aleyrodidae) populations in tomato fields from Tokat, Turkey

Year 2020, Volume: 44 Issue: 3, 319 - 331, 01.09.2020

Tarık Balkan , Kenan Kara

https://doi.org/10.16970/entoted.650742

Cited By: 2

Abstract

Bemisia tabaci (Genn., 1889) (Hemiptera: Aleyrodidae) is one of the most important agricultural pests in Turkey and in the world. This polyphagous pest is a highly efficient vectors of plant viruses and has the ability to rapidly develop resistance to diverse range of insecticides, hence controlling this pest is problematic. In this study, bioassays and biochemical tests were conducted to determine resistance to neonicotinoid in B. tabaci populations collected in 2017-2018 from Tokat (Turkey). According to the adult test results, resistance ratios for acetamiprid, imidacloprid and thiamethoxam were 5.64-16.8, 10.0-30.9 and 4.01-14.9, respectively. The highest resistance ratio for acetamiprid and thiamethoxam in the Pazar population were 16.8 and 14.9, respectively. The highest resistance ratio to imidacloprid was 30.9 in the TOGU campus population. According to the nymph test results, resistance ratios for acetamiprid, imidacloprid and thiamethoxam were 2.96-8.60; 4.29-8.74 and 2.48-4.88, respectively. Enzyme analysis revealed statistically higher metabolic resistance. Maximum enzyme activities were 4.37 and 3.79 pmol/min/mg protein for cytochrome P450 monooxygenase in TOGU campus and Pazar populations, respectively.

Keywords

Acetamiprid, Bemisia tabaci, imidacloprid, insecticide resistance, thiamethoxam

Supporting Institution

Tokat Gaziosmanpasa University Scientific Research Projects Coordination Unit

Project Number

2016/45

Thanks

We are grateful to Tokat Gaziosmanpaşa University Scientific Research Projects Coordination Unit (project number 2016/45) for financial aid.

Tokat (Türkiye) domates alanlarındaki Bemisia tabaci (Genn., 1889) (Hemiptera: Aleyrodidae) ergin ve nimf popülasyonlarında neonikotinoid direnci

Abstract

Bemisia tabaci (Genn., 1889) (Hemiptera: Aleyrodidae) Türkiye ve dünyadaki en önemli tarım zararlılarından biridir. Bu polifag zararlı oldukça etkili bir bitki virüs vektörüdür ve çeşitli insektisitlere karşı hızla direnç geliştirme kabiliyetine sahiptir, bu yüzden zararlıyı kontrol etmek zordur. Bu çalışma 2017-2018 yıllarında Tokat (Türkiye)'tan toplanan farklı B. tabaci popülasyonlarının neonikotinoid grubu insektisitlere karşı direnç durumunu biyoassay ve biyokimyasal yöntemlerle belirlemek amacıyla yapılmıştır. Ergin testlerinde ortaya çıkan sonuçlara göre acetamiprid, imidacloprid ve thiamethoxam için direnç oranları, sırasıyla 5.64-16.8; 10.0-30.9 ve 4.01-14.9 arasındadır. Acetamiprid ve thiamethoxam için en yüksek direnç oranı, Pazar popülasyonunda sırasıyla 16.8 ve 14.9 ’dur. Imidacloprid’e en yüksek direnç ise TOGU kampüs popülasyonunda 30.9’dur. Nimf testlerinde ortaya çıkan sonuçlara göre acetamiprid, imidacloprid ve thiamethoxam için direnç oranları, sırasıyla 2.96-8.60; 4.29-8.74 ve 2.48-4.88 arasındadır. Enzim analizi istatistiki anlamda yüksek metabolik direnci ortaya çıkarmıştır. Her üç insektisit içinde, TOGU kampüs ve Pazar popülasyonlarında en yüksek monooksigenaz P450 enzim aktivitesi sırasıyla 4.37 ve 3.79 pmol/dk/mg protein bulunmuştur.

Keywords

Acetamiprid, Bemisia tabaci, imidacloprid, insektisit direnci, thiamethoxam

Project Number

2016/45

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