Eikosanoid biyosentezi inhibitörlerinin Galleria mellonella (Linnaeus) larvalarının hemolenf protein profiline etkisi

Year 2011, Volume: 35 Issue: 3, 397 - 406, 01.06.2011

Pavel Hyrl Ender Büyükgüzel Kemal Büyükgüzel

Abstract

Keywords

Eikosanoidler, eskuletin, Galleria mellonella, hemolenf, protein profili

Effect of eicosanoid biosynthesis inhibitors on the haemolymph protein profile of Galleria mellonella (Linnaeus) larvae

Abstract

Eicosanoids mediate insects cellular and humoral immune reactions and stress responses. Function of these mediators can be specifically blocked using different eicosanoid biosynthesis inhibitors (EBIs). Effects of EBIs on total haemolymph protein composition have not been extensively studied in insects. We posed the hypothesis that eicosanoids also mediate physiological homeostasis by regulating protein profiles involved in stress response and other defensive reactions. To test this idea,we reared greater wax moth Galleria mellonella (Linnaeus,1758) (Lepidoptera: Pyralidae) larvae on artificial diets containing 0.001, 0.01, 0.1 or 1.0% of specific EBIs with different mode of action: Esculetin, dexamethasone and phenidone. Feeding larvae with esculetin caused significantly dose-dependent changes in 45 kDa protein fraction (one of 16 proteins detected) using sodium dodecyl-sulphate polyacrylamide gradient gel electrophoresis followed by silver staining. Other main haemolymph proteins, lipophorins (ApoLP-I) and storage proteins, were not affected by EBIs treatments. Dexamethasone and phenidone caused no significant differences in detected protein fractions. We infer from these findings that eicosanoids, at least lipoxygenase products, have been implicated in the protein composition of insect tissues as structural and functional concept. Although it has not yet been possible to use directly EBIs for insect pest control, our results bring new data to understand physiological signaling systems in insects

Keywords

-

References

• Aragno, M., S. Parola, E. Rignardello, R. Manti, S. Betteto, E. Tamagno, O. Danni & G. Boccuzzi, 2001. Oxidative stress and eicosanoids in the kidneys of hyperglycemic rats treated with dehydroepiandrosterone. Free Radical Biology and Medicine, 31: 935–942.
• Bronskill, J., 1961. A cage to simplify the rearing of the greater wax moth, Galleria mellonella (Pyralidae). Journal Lepidopterist‘s Society, 15: 102-104.
• Büyükgüzel, E., H. Tunaz, D. Stanley & K. Büyükgüzel, 2007. Eicosanoids mediate Galleria mellonella cellular immune response to viral infection. Journal of Insect Physiology, 53: 99-105.
• Dean, P., J. C. Gadsden, E. H. Richards, J. P. Edwards, A. K. Charnley & S. E. Reynolds, 2002. Modulation by eicosanoid biosynthesis inhibitors of immune responses by the insect Manduca sexta to the pathogenic fungus Metarhizium anisopliae. Journal of Invertebrate Patholology, 79: 3-101.
• Downer, R. G., S. J. Moore, W. Diehl-Jones & C. A. Mandato, 1997. The effects of eicosanoid biosynthesis inhibitors on prophenoloxidase activation, phagocytosis and cell spreading in Galleria mellonella. Journal of Insect Physiology, 43: 1-8.
• Durmuş, Y, E. Büyükgüzel, B. Terzi, H. Tunaz, D. Stanley & K. Büyükgüzel, 2008. Eicosanoids mediate melanotic nodulation reactions to viral infection in larvae of the parasitic wasp, Pimpla turionellae. Journal of Insect Physiology, 54: 17-24.
• Figueiredo, M. B., E. S. Garcia & P. Azambuja, 2008. Blockades of phospholipase A2 and platelet-activating factor receptors reduce the hemocyte phagocytosis in Rhodnius prolixus: In vitro experiments. Journal of Insect Physiology, 54: 344-350.
• García Gil de Muňoz, F. L., J. Martínez-Barnetche, H. Lanz-Mendoza, M. H. Rodríguez & F. C. Hernández-Hernández, 2008. Prostaglandin E2 modulates the expression of antimicrobial peptides in the fat body and midgut of Anopheles albimanus. Archives of Insect Biochemistry and Physiology, 68: 14-25.
• Godlewski, J., B. Kłudkiewicz, K. Grzelak & B. Cymborowski, 2001. Expression of larval hemolymph proteins (Lhp) genes and protein synthesis in the fat body of greater wax moth (Galleria mellonella) larvae during diapause. Journal of Insect Physiology, 47: 759–766.
• Halwani, A. E., D. F. Niven & G. B. Dunphy, 2001. Apolipophorin-III in the greater wax moth, Galleria mellonella (Lepidoptera: Pyralidae). Archives of Insect Biochemistry and Physiology, 48: 135-143.
• Hyršl, P., E. Büyükgüzel & K. Büyükgüzel, 2008. Boric acid-induced effects on protein profiles of Galleria mellonella hemolymph and fat body. Acta Biologica Hungarica, 59: 281-288.
• Kirkeby, S., D. Moe & T. C. Bog-Hansen, 1993. The silver staining procedure of sodium dodecyl sulphate-gels may be accelerated by shortening fixation time. Electrophoresis, 14: 51-55.
• Laemmli, U. K, 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685.
• Mandato, C. A., W. L. Diehl-Jones, S. J. Moore & R. G. H Downer, 1997. The Effects of eicosanoid biosynthesis inhibitors on prophenoloxidase activation, phagocytosis and cell spreading in Galleria mellonella. Journal of Insect Physiology, 43: 1-8.
• Merchant, D., R. L. Ertl, S. I. Rennard, D. W. Stanley & J. S. Miller, 2008. Eicosanoids mediate insect hemocyte migration. Journal of Insect Physiolology, 54: 215–221.
• Miao, Y. & L. Jiang, 2003. Changes in growth and lipid profiles of silk gland, midgut biochemical composition of silkworm, Bombyx mori L. on exposure to prostaglandin F2α. Prostaglandins & Other Lipid Mediators, 70: 331-338.
• Miller, S. G. & D. L. Silhacek, 1982. The synthesis and uptake of heamolymph storage proteins by the fat body of the greater wax moth Galleria mellonella (L.). Insect Biochemistry, 12: 293-300.
• Miller, J. S. & D. W. Stanley, 2001. Eicosanoids mediate microaggregation reactions to bacterial challenge in isolated insect hemocyte preparations. Journal of Insect Physiology, 47: 1409-1417.
• Morishima, I., Y. Yamano, K. Inoue & N. Matsuo, 1997. Eicosanoids mediate induction of immune genes in the fat body of the silkworm, Bombyx mori. FEBS Letters, 419: 83-86.
• Negishi, M. & H. Katoh, 2002. Cyclopentenone prostaglandin receptors. Prostaglandins & Other Lipid Mediators, 69: 611–617.
• Sandstrom, P. A., D. Pardi, D. M. Terrian, T. M. Folks & T. M. Buttke, 1995. Lipid hydroperoxide-induced apoptosis: lack of inhibition by blc-2 over-expression. FEBS Letters, 365: 66-70.
• Stanley, D. W., 2006. Prostaglandins and other eicosanoids in insects: biological significance. Annual Review of Entomology, 51: 25-44.
• Stanley, D. W., C. Goodman, S. An, A. Mcintosh & Q. Song, 2008. Prostaglandins A1 and E1 influence gene expression in an established insect cell line (BCIRL- HzAM1 cells). Insect Biochemistry and Molecular Biology, 38: 275-284.
• Stanley, D. & M. Shapiro, 2007. Eicosanoid biosynthesis inhibitors increase the susceptibility of Lymantria dispar to nucleopolyhedrovirus LdMNPV. Journal of Invertebrate Pathology, 95 (2): 119–124.
• Tunaz, H., 2006. Eicosanoid biosynthesis inhibitors influence mortality of Pieris brassicae larvae co-injected with fungal conidia. Archives of Insect Biochemistry and Physiology, 63: 93-100.
• Wiesner, A., S. Losen, P. Kopacek, C. Weise & P. Götz, 1997. Isolated apolipophorin III from Galleria mellonella stimulates the immune reactions of this insect. Journal of Insect Physiology, 43: 383-391.