Protein patterns in the salivary gland of the sunn pest, Eurygaster integriceps (Put.) (Hemiptera: Scutelleridae)

Year 2012, Volume: 36 Issue: 2, 215 - 224, 01.04.2012

Mohammad Saadati Bezdı Reza F. Pourabad Mahmoud Toorchı Nosratollah Zarghamı Setsuko Komatsu 215-223

Abstract

Keywords

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Süne [Eurygaster integriceps (Put.) (Hemiptera: Scutelleridae)]'nin salya bezindeki protein desenleri

Abstract

Hemipteradaki salya bezleri, ağız dışındaki sindirimde etkili olan, böcek-bitki ilişkileri açısından önemli proteinleri içerir. Bu çalışmada Süne’nin beşinci dönem nimfleri ve ergin bireylerinde salya bezlerinin protein dokularının karşılaştırılmasında protomiks tekniklerinden yararlanılmıştır. Belirlenen 139 leke şeklindeki desenlerden 31 tanesi, proteinlerin tanılanması için seçilmiştir. 13 protein lekesi iki yaşam dönemi arasında farklı olarak belirlenmiştir. Ergin bireylerde tripsin, arginin kinaz ve fofolipaz A2 artış gösterirken, beşinci dönem nimflerde ise kimotripsin, arjinin metiltransferaz ve endonukleaz salyası daha yüksek miktarda gözlenmiştir. Diğer 18 leke salya bezinin protein haritasını oluşturmak için seçilmiştir. Beşinci dönem nimfler ile ergin bireylerde; ssp3 serin proteazları, alfa-amilaz, glukoz dehidrojenaz, vakuolar H+ATPase ve trombostasin-3’ün aynı oranlarda olduğu belirlenmiştir. Proteinlerin tanılanması ve salya bezlerindeki fizyolojik rollerinin belirlenmesi Süne-buğday etkileşimlerini aydınlatmak için önemli bir adımdır

Keywords

Salya bezi, proteomikler, pis kokulu böcek, enzim, sindirim

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