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Avcı akar Neoseiulus californicus (Acari: Phytoseiidae) poülasyonlarının üç farklı akarisite karşı duyarlılık ve detoksifikasyon enzim düzeylerinin belirlenmesi

Year 2013, Volume: 37 Issue: 1, 105 - 116, 01.02.2013

Sibel Yorulmaz Salman Recep Ay (105-116)

Abstract

This study aimed to determine the sensitivity levels of Neoseiulus californicus (McGregor) (Acari: Phytoseiidae) populations collected from the apple orchards in Isparta in 2011 to etoxazole, hexythiazox and spirodiclofen by using bioassay methods. LC values of N. californicus populations were determined by using a spray tower with the leaf disk method. According to LC values, compared susceptible populations, resistance of Atabey-1, Atabey-2, Ağılköy-1, Ağılköy-2, Gönen and Yalvaç populations collected from the orchard in 2011 is determined to be 10.14, 11.34, 11.75, 14.41, 9.85 and 9.66 fold to etoxazole; to be 6.35, 3.96, 9.33, 8.35, 7.34 and 9.79 fold to hexythiazox; and to be 4.61, 4.36, 5.86, 4.82, 4.10 and 7.08 fold, respectively. Moreover, the effect of the PBO, IBP and DEM synergists on pesticides was examined. Enzymes of S-transferase (GST), monooxygenases (P450) and acetylcholinesterase (AChE) in the popluations were determined by using the kinetic method; and the enzyme of esterase was determined by using the electrophoresis and kinetic methods. The determined enzyme activity ranges of esterase, glutathion S-transferaz (GST), monooxygenases (P450) ve asetilkolinesteraz (AChE) were between from 7.130 to 11.788, from 2.07 to 3.47, from 0.0223 to 0.0695 and from 0.0211 to 0.0243 mOD/min/mg proteins, respectively.

Keywords

Neoseiulus californicus acaricide resistance sinergist detoxification

Determination of the susceptibility and detoxification enzyme levels of the predatory mite Neoseiulus californicus (Acari: Phytoseiidae) populations against three different acaricides

Year 2013, Volume: 37 Issue: 1, 105 - 116, 01.02.2013

Sibel Yorulmaz Salman Recep Ay (105-116)

Abstract

Bu çalışmada, Isparta ili elma bahçelerinden 2011 yılında toplanan Neoseiulus californicus (McGregor)(Acari: Phytoseiidae) popülasyonlarının etoxazole, hexythiazox ve spirodiclofen’e karşı duyarlılık düzeyleri biyoassay yöntemlerle belirlenmiştir. N. californicus popülasyonlarının LC değeri ilaçlama kulesi kullanılarak yaprak disk metodu ile belirlenmiştir. 2011 yılında elma bahçelerinden toplanan Atabey-1, Atabey-2, Ağılköy-1, Ağılköy-2, Gönen ve Yalvaç isimli N. californicus popülasyonlarında etoxazole karşı sırasıyla 10.14, 11.34, 11.75, 14.41, 9.85 ve 9.66 kat; hexythiazox’a karşı 6.35, 3.96, 9.33, 8.35, 7.34 ve 9.79 kat ve spirodiclofen’e karşı 4.61, 4.36, 5.86, 4.82, 4.10 ve 7.08 kat direnç belirlenmiştir. Ayrıca N. californicus popülasyonlarında PBO, IBP ve DEM sinerjistlerinin ilaçlar ile sinerjistik etkileri incelenmiştir. N. californicus popülasyonlarında glutathion S-transferaz (GST) ve asetilkolinesteraz (AChE) enzimleri kinetik yöntemle, esteraz enzimi elektroforez ve kinetik yöntemlerle belirlenmiştir. Esteraz, glutathion S-transferaz (GST), monooksigenaz (P450) ve asetilkolinesteraz (AChE) enzim aktiviteleri sırasıyla 7.13011.788, 2.07-3.47, 0.0223-0.0695 ve 0.0211-0.0243 mOD/min/mg protein olarak belirlenmiştir.

Keywords

Neoseiulus californicus akarisit direnç sinerjist detoksifikasyon enzimleri

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Details

Primary Language Turkish
Journal Section Articles
Authors

Sibel Yorulmaz Salman This is me

Recep Ay (105-116) This is me

Publication Date February 1, 2013
Submission Date February 8, 2014
Published in Issue Year 2013 Volume: 37 Issue: 1

Cite

APA Salman, S. Y. ., & (105-116), R. A. . (2013). Determination of the susceptibility and detoxification enzyme levels of the predatory mite Neoseiulus californicus (Acari: Phytoseiidae) populations against three different acaricides. Turkish Journal of Entomology, 37(1), 105-116.
AMA Salman SY, (105-116) RA. Determination of the susceptibility and detoxification enzyme levels of the predatory mite Neoseiulus californicus (Acari: Phytoseiidae) populations against three different acaricides. TED. February 2013;37(1):105-116.
Chicago Salman, Sibel Yorulmaz, and Recep Ay (105-116). “Determination of the Susceptibility and Detoxification Enzyme Levels of the Predatory Mite Neoseiulus Californicus (Acari: Phytoseiidae) Populations Against Three Different Acaricides”. Turkish Journal of Entomology 37, no. 1 (February 2013): 105-16.
EndNote Salman SY, (105-116) RA (February 1, 2013) Determination of the susceptibility and detoxification enzyme levels of the predatory mite Neoseiulus californicus (Acari: Phytoseiidae) populations against three different acaricides. Turkish Journal of Entomology 37 1 105–116.
IEEE S. Y. . Salman and R. A. . (105-116), “Determination of the susceptibility and detoxification enzyme levels of the predatory mite Neoseiulus californicus (Acari: Phytoseiidae) populations against three different acaricides”, TED, vol. 37, no. 1, pp. 105–116, 2013.
ISNAD Salman, Sibel Yorulmaz - (105-116), Recep Ay. “Determination of the Susceptibility and Detoxification Enzyme Levels of the Predatory Mite Neoseiulus Californicus (Acari: Phytoseiidae) Populations Against Three Different Acaricides”. Turkish Journal of Entomology 37/1 (February 2013), 105-116.
JAMA Salman SY, (105-116) RA. Determination of the susceptibility and detoxification enzyme levels of the predatory mite Neoseiulus californicus (Acari: Phytoseiidae) populations against three different acaricides. TED. 2013;37:105–116.
MLA Salman, Sibel Yorulmaz and Recep Ay (105-116). “Determination of the Susceptibility and Detoxification Enzyme Levels of the Predatory Mite Neoseiulus Californicus (Acari: Phytoseiidae) Populations Against Three Different Acaricides”. Turkish Journal of Entomology, vol. 37, no. 1, 2013, pp. 105-16.
Vancouver Salman SY, (105-116) RA. Determination of the susceptibility and detoxification enzyme levels of the predatory mite Neoseiulus californicus (Acari: Phytoseiidae) populations against three different acaricides. TED. 2013;37(1):105-16.