Per os feeding with antisera specific to peritrophic matrix proteins stimulates larval growth in Mamestra configurata Walker (Lepidoptera: Noctuidae)

Year 2013, Volume: 37 Issue: 2, 203 - 212, 01.04.2013

Umut Toprak Doug Baldwın Dwayne D. Hegedus Martin Erlandson (203-211)

Abstract

Peritrophic matrix (PM) is an acellular, porous sheath composed of chitin and proteins that lines the midgut epithelial cells. The PM serves as a barrier against food particles, pathogens and toxins. Several studies in dipteran systems revealed that antibodies specific to PM proteins retard insect development by binding to their target antigens in the PM and blocking PM pores. To test whether antisera specific to PM proteins also inhibit larval growth in a lepidopteran system, antisera specific to chitin deacetylase 1, insect intestinal mucin 2, insect intestinal mucin 4 and PM protein 1 were fed to 2nd instar Mamestra configurata Walker (Lepidoptera: Noctuidae) larvae, a major pest of Brassica plants in North America. Interestingly, all larvae feeding on antisera gained more weight than the larvae feeding on the diet containing non-immune sera or no antiserum. The anti-McPM1 and anti-McIIM4 antisera treatments showed the highest larval weight gains, followed by the anti-McCDA1 and anti-McIIM2 antisera treatments. The interaction of treatment with time was found significant by the 6th day and the interaction of concentration with time was found significant only by the 12th day. No difference was found between the larval weights from all treatments at concentrations of 1 or 4%.

Keywords

Antiserum, feeding, peritrophic matrix, peritrophin, chitin deacetylase

Peritrofik matriks proteinlerine spesifik antiserumların ağız yoluyla yedirilmesinin Mamestra configurata Walker (Lepidoptera: Noctuidae) larva gelişimine etkisi

Abstract

Peritrofik matriks (PM) mide epitel hücreleri boyunca uzanan, kitin ve proteinden oluşan gözenekli ve hücresiz bir yapıdır. PM besin partikülleri, patojenler ve toksinlere karşı bir bariyer görevi görmektedir. Dipter sistemlerdeki çeşitli araştırmalar PM proteinlerine spesifik antibadilerin PM’deki hedef antijenlerine bağlanıp PM gözeneklerini tıkayarak böcek gelişimini geciktirdiğini göstermiştir. PM proteinlerine spesifik antibadilerin lepidopter bir sistemde de larva gelişimini engelleyip engellemediğinin belirlenmesi amacıyla, kitin deasetilaz 1, böcek barsak musini 2, böcek barsak musini 4 ve PM proteini 1 proteinlerine spesifik antiserumlar, Kuzey Amerika’daki Brassica bitkilerinin ana zararlısı durumunda olan Mamestra configurata Walker (Lepidoptera: Noctuidae)’nın 2. dönem larvalarına yedirilmiştir. İlginç olarak bu larvalar, antiserum içermeyen veya normal serum içeren besin üzerinde beslenen larvalara göre daha fazla ağırlık kazanmıştır. Anti-McPM1 and anti-McIIM4 antiserum denemeleri en yüksek larva ağırlık artışına neden olurken, anti-McCDA1 and anti-McIIM2 antiserum denemeleri daha az ağırlık artışına neden olmuştur. Antiserum-zaman ilişkisi 6. günden itibaren önemli bulunurken, konsantrasyon-zaman ilişkisi sadece 12. günde önemli bulunmuştur. Tüm antiserum uygulamalarının % 1 veya % 4’lük konsantrasyonlarındaki larva ağırlık artışları arasındaki fark önemli bulunmamıştır.

Keywords

Antiserum, beslenme, peritrofik matriks, peritrofin, kitin deasetilaz

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