Comparison of hermaphrodites of hybrid Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH strain and its parents on reproduction capacity

Year 2021, Volume: 45 Issue: 2, 185 - 191, 01.06.2021

Tufan C Ulu , Gizem Özbudak Elif Özge Düzenli Şeyma Hümeyra Çakır Alper Susurluk

https://doi.org/10.16970/entoted.858061

Cited By: 1

Abstract

Entomopathogenic nematodes (EPNs) are microscopic roundworms used in biocontrol. EPNs are obligate insect parasites, they live in soil, and they are especially effective against soilborne insects. They are a good alternative to chemical pesticides thanks to their advantages, such as prolonged longevity, broad host range and mass production suitability. However, EPNs cannot compete with chemical pesticides due to high production costs and short shelf life. The aim of this study was to determine the reproduction capacity of the Turkish hybrid Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH strain and then compare it with its parents to improve its liquid culture yield. In this way, it is aimed to reveal the effects behind the high reproduction potential of the hybrid HBH strain. All experiments were performed at Bursa Uludağ University, Faculty of Agriculture, Department of Plant Protection, in 2020. All cadavers were periodically dissected, hermaphrodites were counted and their body lengths were measured. Compared to its parents, the hybrid HBH strain had greater hermaphrodite counts, with mean 66 individuals within 12 days, and hermaphrodite body length, with mean 3.88 mm. The results obtained from this study should provide information for commercial EPN production development.

Keywords

Body length, hermaphrodite, Heterorhabditis bacteriophora, in vitro, reproduction capacity

Supporting Institution

TUBITAK (The Scientific and Technological Research Council of Turkey)

Project Number

TOVAG 219O370

Thanks

This study was financially supported by the TUBITAK (The Scientific and Technological Research Council of Turkey), Project number: TOVAG 219O370.

Hibrit Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH ırkı ve ebeveynlerinin hermafroditlerinin üreme kapasitelerinin karşılaştırılması

Abstract

Entomopatojen nematodlar (EPN) biyolojik mücadelede kullanılan mikroskobik ölçülerdeki yuvarlak solucanlardır. EPN’ler zorunlu böcek parazitleri olup, toprak altında yaşamakta ve toprak kökenli böceklere karşı etkili olmaktadırlar. Uygulamalarının kolay olması, uzun sürekli etkinliği, geniş konukçu aralığı ve kitle üretimi yapılabilmesi gibi avantajları sayesinde kimyasal ilaçlara iyi bir alternatif olmaktadırlar. Bununla beraber, yüksek üretim maliyetleri ve kısa raf ömrü nedeniyle EPN’ler kimyasal ilaçlar ile rekabet edememektedir. Bu çalışma, sıvı kültürdeki üretim verimini artırmak amacıyla Türk hibriti Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH ırkının üreme kapasitesini belirlemek ve ebeveynleri ile karşılaştırmak amacıyla yapılmıştır. Bu sayede hibrit HBH ırkının yüksek üreme potansiyelinin arkasında yatan etkilerin ortaya çıkarılması hedeflenmiştir. Tüm denemeler Bursa Uludağ Üniversitesi Ziraat Fakültesi Bitki Koruma Bölümü’nde 2020 yılında gerçekleştirilmiştir. Bütün kadavralar periyodik olarak disekte edilmiş, hermafroditler sayılmış ve vücut uzunlukları ölçülmüştür. Ebeveynleri ile karşılaştırıldığında, hibrit HBH ırkı, 12 günde ortalama 66 hermafrodit birey ile hermafrodit sayısında ve ortalama 3.88 mm ile hermafrodit vücut uzunluğu açısından daha yüksek değerlere sahip olmuştur. Bu çalışma ile elde edilen sonuçlar, ticari EPN üretim çalışmalarına bilgi sağlayacaktır.

Keywords

Vücut uzunluğu, hermafrodit, Heterorhabditis bacteriophora, in vitro, üreme kapasitesi

Project Number

TOVAG 219O370

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