The influence of juvenile hormone analogue, fenoxycarb on the midgut remodeling in Bombyx mori (L., 1758) (Lepidoptera: Bombycidae) during larval-pupal metamorphosis

Year 2011, Volume: 35 Issue: 2, 179 - 194, 30.06.2011

Ebru Goncu Osman Parlak

Abstract

During larval-pupal metamorphosis, midgut tissues of holometabolous insects undergo remodeling and this remodeling process involves two metamorphic events. Degeneration of larval midgut occurs via programmed cell death and pupal midgut develops from regenerative (stem) cells. All metamorphic events in insects are regulated by ecdysone and juvenile hormone. Molecular mechanisms of ecdysone are well known but action mechanism of juvenile hormone is not well understood. Fenoxycarb, 0-ethyl N-(2-(4-pheoxyphenoxy)-ethyl) carbamate has been previously shown one of the most potent juvenile hormone analogue against a variety of insect species and widely used for investigations the mechanisms of juvenile hormone. In this study, we aimed to analyze the metamorphic events in the midgut of Bombyx mori Linnaeus, 1758 and investigate the effects of fenoxycarb on this period. For this purpose, we applied two different doses of fenoxycarb (1ng/10µl and 10ng/10µl) topically on day 0 of fifth instar larvae. We followed the progression of remodeling processes with morphological observations and analyzed programmed cell death specific proteins, caspase 3 for apoptosis and acid phosphatase for autophagic cell death in control and experiment groups. Our results indicated that, programmed cell death of larval midgut has morphologic characteristics of apoptosis and caspase activation occurs during this period and juvenile hormone analogue fenoxycarb inhibits or delays remodeling process in dose dependent manner.

Keywords

Apoptosis, autophagic cell death, Bombyx mori, fenoxycarb, midgut

Supporting Institution

The research was supported by Ege University

Project Number

2009 Science 021

Bombyx mori’ L., 1758 (Lepidoptera: Bombycidae) de larval-pupal metamorfoz süresince orta barsaktaki yeniden şekillenme sürecine juvenil hormon analoğu fenoxycarb’ın etkisi

Abstract

Larval-pupal metamorfoz boyunca, tam başkalaşım geçiren böceklerin orta barsakları yeniden şekillenme sürecine girer ve bu süreç 2 metamorfik olayı içerir. Larval orta barsağın dejenerasyonu programlanmış hücre ölümü ile meydana gelirken pupal orta barsak rejeneratif (kök) hücrelerden oluşur. Böceklerdeki tüm metamorfik olaylar ekdizon ve juvenil hormon tarafından kontrol edilir. Ekdizonun moleküler mekanizması gayet iyi bilinmekle birlikte juvenil hormonun moleküler mekanizması henüz tam olarak anlaşılamamıştır. Fenoksikarb, O-ethyl N-(2-(4-pheoxyphenoxy)-ethyl) karbamat’ın pek çok böcek türünde etkili juvenil hormon analoglarından birisi olduğu ortaya konulmuştur ve juvenil hormonun mekanizmasına ilişkin çalışmalarda sıklıkla kullanılmaktadır. Bu çalışmada, Bombyx mori Linnaeus, 1758’nin orta barsağındaki metamorfik olayların analiz edilmesi ve bu süreçte fenoksikarb’ın etkisinin araştırılması amaçlanmıştır. Bu amaçla, beşinci larval döneminin 0. gününde iki farklı dozda fenoksikarb (1ng/10µl and 10ng/10µl) topikal olarak uygulanmıştır. Yeniden şekillenme sürecinin ilerleyişini morfolojik gözlemler ve programlanmış hücre ölümüne spesifik proteinlerin - apoptosis için kaspaz 3 ve otofajik hücre ölümü için asit fosfataz- kontrol ve deney gruplarında analizi ile takip edilmiştir. Sonuçlar larval orta barsakta programlanmış hücre ölümünün apoptozisin morfolojik karakterlerine sahip olduğunu, bu period boyunca kaspaz aktivasyonunun meydana geldiğini ve juvenil hormon analoğu fenoksikarb’ın doza bağlı olarak yeniden şekillenme sürecini geciktirdiğini veya engellediğini göstermiştir.

Keywords

Apoptozis, otofajik hücre ölümü Bombyx mori, fenoksikarb, orta barsak

Project Number

2009 Science 021

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