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Translocation of clothianidin to guttation fluid and its potential impact on honey bee, Apis mellifera anatoliaca Maa, 1953 (Hymenoptera: Apidae)

Year 2021, Volume: 45 Issue: 4, 511 - 522, 15.12.2021

Melis Yalçın , İlknur Kapiz Mustafa Kösoğlu , Onur Koca , Nalan Turgut , Erkan Topal , Perihan Binnur Kurt Karakuş , Levent Atatanır , Serhan Mermer , Merve Özkaleli Akçetin , Jens Pistorius Cafer Turgut

https://doi.org/10.16970/entoted.1017479
Cited By: 3

Abstract

Honey bees, Apis mellifera anatoliaca Maa, 1953 (Hymenoptera: Apidae) forage water from guttation fluid so transported neonicotinoid insecticides in guttation fluid poses a risk to the bees. The first aim of this study was to determine the toxicity and risk of clothianidin to honey bees. In addition, the changes of clothianidin residue in the guttation fluid of maize plants in Turkey were determined in 2018 and 2019. Also, the toxicity of guttation fluid collected from the maize plants to bees was determined in ecotoxicological tests. The acute oral LD50 of clothianidin to honey bees in the first 24 h was 1.80 ng bee-1 and residue analysis demonstrated that honey bees were exposed to clothianidin concentration in guttation fluid ranging from 0.02 to 6.0 mg L-1 with mortality ranging between 80 and 100%. As the measured concentration of clothianidin in guttation fluid can lead to the mortality of honey bees, present study indicates that clothianidin, and possibly related pesticides in treated maize seed poses a risk to honey bees. Future studies are needed to determine the scale and distribution of this risk in Turkey.

Keywords

Clothianidin exposure risk guttation honey bees residue

Supporting Institution

Scientific and Technological Research Council of Turkey (TÜBİTAK), Project No: TOVAG 118O522 for 2019 field studies and Aydın Adnan Menderes University Research Funds, Project No: ZRF-18016 for 2018

Project Number

Scientific and Technological Research Council of Turkey (TÜBİTAK), Project No: TOVAG 118O522; Aydın Adnan Menderes University Research Funds, Project No: ZRF-18016

Thanks

This work was supported by the Scientific and Technological Research Council of Turkey (TÜBİTAK), Project No: TOVAG 118O522 for 2019 field studies and Aydın Adnan Menderes University Research Funds, Project No: ZRF-18016 for 2018 field studies.

References

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  • Brandt, A., K. Grikscheit, R. Siede, R. Grosse, M. D. Meixner & R. Büchler, 2017. Immunosuppression in honeybee queens by the neonicotinoids thiacloprid and clothianidin. Scientific Reports, 7 (4673): 1-12.
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Clothianidinin gutasyon sıvısında taşınımı ve bal arısı, Apis mellifera anatoliaca Maa, 1953 (Hymenoptera: Apidae) üzerindeki etkisinin belirlenmesi

Year 2021, Volume: 45 Issue: 4, 511 - 522, 15.12.2021

Melis Yalçın , İlknur Kapiz Mustafa Kösoğlu , Onur Koca , Nalan Turgut , Erkan Topal , Perihan Binnur Kurt Karakuş , Levent Atatanır , Serhan Mermer , Merve Özkaleli Akçetin , Jens Pistorius Cafer Turgut

https://doi.org/10.16970/entoted.1017479
Cited By: 3

Abstract

Öz
Bal arılarının, Apis mellifera anatoliaca Maa, 1953 (Hymenoptera: Apidae) gutasyon sıvısından su ihtiyacını karşılaması sırasında gutasyon sıvısında tohum ilacı olarak kullanılan neonicotinoid insektisitlerin taşınması arılarda risk yaratmaktadır. Bu çalışmanın ilk amacı clothianidinin arılara toksisitesi ve riskinin belirlenmesidir. Bunun yanında 2018 ve 2019 yıllarında Türkiye’de mısır bitkisindeki gutasyon sıvısında clothianidin kalıntısının değişimleri belirlenmiştir. Ayrıca mısır bitkisinden toplanan gutasyon sıvılarının arılara toksisitesinde yapılan ekotoksikolojik testlerde tespit edilmiştir. Clothianidinin ilk 24 saatteki bal arılarına olan toksisitesi incelendiğinde ortalama akut oral LD50 değeri 1.80 ng arı-1 olarak saptanmıştır. Bununla birlikte kalıntı analizleri sonucunda bal arılarının gutasyon sıvısında 0.02 mg L-1 ile 6 mg L-1 arasında değişen clothianidin konsantrasyonuna maruz kaldığı tespit edilmiş olup bal arılarının ölüm oranının %80 ile %100 arasında değiştiği gözlenmiştir. Gutasyon sıvısında ölçülen clothianidin konsantrasyonu bal arılarının ölümüne yol açabileceğinden, bu çalışma, mısır tohumlarına uygulanan clothianidin ve benzer grupta olan pestisitlerin, bal arıları için risk oluşturduğunu göstermektedir. Bu riskin Türkiye’deki miktarını ve dağılımını belirlemek için gelecekte yapılacak çalışmalara ihtiyaç vardır.

Keywords

Clothianidin maruziyet riski gutasyon bal arıları kalıntı

Project Number

Scientific and Technological Research Council of Turkey (TÜBİTAK), Project No: TOVAG 118O522; Aydın Adnan Menderes University Research Funds, Project No: ZRF-18016

References

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  • Akyol, E. & O. Kaftanoğlu, 2001. Colony characteristics and the performance of Caucasian (Apis mellifera caucasica) and Mugla (Apis mellifera anatoliaca) bees and their reciprocal crosses. Journal of Apicultural Research, 40 (3): 11-15.
  • Alkassab, A. T. & W. H. Kirchner, 2016. Impacts of chronic sublethal exposure to clothianidin on winter honeybees. Ecotoxicology, 25 (5): 1000.
  • Anastassiades, M., S. J. Lehotay, D. Stajnbaher & F. J. Schenck, 2003. Fast and easy multiresidue method employing acetonitrile extraction/partitioning and “dispersive solid-phase extraction” for the determination of pesticide residues in produce. Journal of AOAC International, 86 (2): 412-431.
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  • Brandt, A., A. Gorenflo, R. Siede, M. Meixner & R. Büchler, 2016. The neonicotinoids thiacloprid, imidacloprid, and clothianidin affect the immunocompetence of honey bees (Apis mellifera L.). Journal of Insect Physiology, 86: 40-47.
  • Brandt, A., K. Grikscheit, R. Siede, R. Grosse, M. D. Meixner & R. Büchler, 2017. Immunosuppression in honeybee queens by the neonicotinoids thiacloprid and clothianidin. Scientific Reports, 7 (4673): 1-12.
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  • Çil, G. İ., S. D. Korkmaz, G. Ozansoy & Ö. Küplülü, 2020. Türkiye’deki bal örneklerinde neonicotinoid varlığının LC-MS/Q-TOF yöntemi ile tespiti. Mehmet Akif Ersoy University Journal of Health Sciences Institute, 8 (1): 11-17. (In Turkish with abstract in English).
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  • EFSA, 2013. Conclusion on the peer review of the pesticide risk assessment for bees for the active substance thiamethoxam. EFSA Journal, 11 (1): 3067.
  • Flores, J. M., V. Gamiz, S. Gil-Lebrero, I. Rodriguez, F. J. Navas, A. I. Garcia-Valcarcel, V. Cutillas, A. R. Fernandez-Alba & M. D. Hernando, 2021. A three-year large-scale study on the risk of honey bee colony exposure to blooming sunflowers grown from seeds treated with thiamethoxam and clothianidin neonicotinoids. Chemosphere, 262 (127735): 1-9.
  • Frommberger, M., J. Pistorius, I. Joachimsmeier & D. Schenke, 2011. ʺGuttation and the risk for honey bee colonies (Apis mellifera L.): a worst case semi-field scenario in maize with special consideration of impact on bee brood and brood development, 71-75ʺ. 11th International Symposium of the ICP-BR Bee Protection Group (2-4 November 2011, Wageningen, The Netherlands), Julius-Kühn-Archiv, 437 pp.
  • Girolami, V., L. Mazzon, A. Squartini, N. Mori, M. Marzaro, A. D. Bernardo, M. Greatti, C. Giorino & A. Tapparo, 2009. Translocation of neonicotinoid insecticides from coated seeds to seedling guttation drops: a novel way of intoxication for bees. Journal of Economic Entomology, 102 (5): 1808-1815.
  • Girolami, V., M. Marzaro, L. Vivan, L. Mazzon, M. Greatti, C. Giorio, D. Marton & A. Tapparo, 2012. Fatal powdering of bees in flight with particules of neonicotinoids seed coating and humidity implication. Journal of Applied Entomology, 136 (1-2):17-26.
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There are 65 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Melis Yalçın 0000-0002-9122-7133

İlknur Kapiz This is me 0000-0001-8045-1837

Mustafa Kösoğlu 0000-0001-6616-089X

Onur Koca 0000-0002-0753-0077

Nalan Turgut 0000-0001-5099-5024

Erkan Topal 0000-0002-1398-4390

Perihan Binnur Kurt Karakuş 0000-0001-6737-3475

Levent Atatanır 0000-0001-5638-6676

Serhan Mermer 0000-0003-2949-6372

Merve Özkaleli Akçetin 0000-0001-5593-7966

Jens Pistorius This is me 0000-0002-7707-1216

Cafer Turgut 0000-0002-6450-5361

Project Number Scientific and Technological Research Council of Turkey (TÜBİTAK), Project No: TOVAG 118O522; Aydın Adnan Menderes University Research Funds, Project No: ZRF-18016
Early Pub Date November 17, 2021
Publication Date December 15, 2021
Submission Date November 5, 2021
Acceptance Date January 20, 2022
Published in Issue Year 2021 Volume: 45 Issue: 4

Cite

APA Yalçın, M., Kapiz, İ., Kösoğlu, M., Koca, O., et al. (2021). Translocation of clothianidin to guttation fluid and its potential impact on honey bee, Apis mellifera anatoliaca Maa, 1953 (Hymenoptera: Apidae). Turkish Journal of Entomology, 45(4), 511-522. https://doi.org/10.16970/entoted.1017479
AMA Yalçın M, Kapiz İ, Kösoğlu M, Koca O, Turgut N, Topal E, Karakuş PBK, Atatanır L, Mermer S, Özkaleli Akçetin M, Pistorius J, Turgut C. Translocation of clothianidin to guttation fluid and its potential impact on honey bee, Apis mellifera anatoliaca Maa, 1953 (Hymenoptera: Apidae). TED. December 2021;45(4):511-522. doi:10.16970/entoted.1017479
Chicago Yalçın, Melis, İlknur Kapiz, Mustafa Kösoğlu, Onur Koca, Nalan Turgut, Erkan Topal, Perihan Binnur Kurt Karakuş, Levent Atatanır, Serhan Mermer, Merve Özkaleli Akçetin, Jens Pistorius, and Cafer Turgut. “Translocation of Clothianidin to Guttation Fluid and Its Potential Impact on Honey Bee, Apis Mellifera Anatoliaca Maa, 1953 (Hymenoptera: Apidae)”. Turkish Journal of Entomology 45, no. 4 (December 2021): 511-22. https://doi.org/10.16970/entoted.1017479.
EndNote Yalçın M, Kapiz İ, Kösoğlu M, Koca O, Turgut N, Topal E, Karakuş PBK, Atatanır L, Mermer S, Özkaleli Akçetin M, Pistorius J, Turgut C (December 1, 2021) Translocation of clothianidin to guttation fluid and its potential impact on honey bee, Apis mellifera anatoliaca Maa, 1953 (Hymenoptera: Apidae). Turkish Journal of Entomology 45 4 511–522.
IEEE M. Yalçın, “Translocation of clothianidin to guttation fluid and its potential impact on honey bee, Apis mellifera anatoliaca Maa, 1953 (Hymenoptera: Apidae)”, TED, vol. 45, no. 4, pp. 511–522, 2021, doi: 10.16970/entoted.1017479.
ISNAD Yalçın, Melis et al. “Translocation of Clothianidin to Guttation Fluid and Its Potential Impact on Honey Bee, Apis Mellifera Anatoliaca Maa, 1953 (Hymenoptera: Apidae)”. Turkish Journal of Entomology 45/4 (December 2021), 511-522. https://doi.org/10.16970/entoted.1017479.
JAMA Yalçın M, Kapiz İ, Kösoğlu M, Koca O, Turgut N, Topal E, Karakuş PBK, Atatanır L, Mermer S, Özkaleli Akçetin M, Pistorius J, Turgut C. Translocation of clothianidin to guttation fluid and its potential impact on honey bee, Apis mellifera anatoliaca Maa, 1953 (Hymenoptera: Apidae). TED. 2021;45:511–522.
MLA Yalçın, Melis et al. “Translocation of Clothianidin to Guttation Fluid and Its Potential Impact on Honey Bee, Apis Mellifera Anatoliaca Maa, 1953 (Hymenoptera: Apidae)”. Turkish Journal of Entomology, vol. 45, no. 4, 2021, pp. 511-22, doi:10.16970/entoted.1017479.
Vancouver Yalçın M, Kapiz İ, Kösoğlu M, Koca O, Turgut N, Topal E, Karakuş PBK, Atatanır L, Mermer S, Özkaleli Akçetin M, Pistorius J, Turgut C. Translocation of clothianidin to guttation fluid and its potential impact on honey bee, Apis mellifera anatoliaca Maa, 1953 (Hymenoptera: Apidae). TED. 2021;45(4):511-22.

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