Influence of dietary indole-3-acetic acid on phenoloxidase and hemolytic activities in Pimpla turionellae L., 1758 (Hymenoptera: Ichneumonidae) and Galleria mellonella L., 1758 (Lepidoptera: Pyralidae) in a host-parasitoid system

Year 2022, Volume: 46 Issue: 2, 149 - 158, 01.06.2022

Gülşah Becerikli Aksan Fevzi Uçkan , Aylin Er

https://doi.org/10.16970/entoted.1039327

Cited By: 2

Abstract

The continuity of food demand has caused modern agriculture to be heavily dependent on chemical inputs. Plant growth regulators (PGRs) are naturally occurring or synthetic compounds that have the potential to control pest insects through their chemosterilant activity. Along with pests, non-target organisms such as parasitoids in agroecological systems are likely to be influenced by indirect contact via their hosts or direct contact with hosts and plants at the tritrophic level. This study demonstrated the dietary effects of PGR indole-3-acetic acid (IAA) on hemolytic activity and phenoloxidase activity in stored product pest Galleria mellonella L., 1758 (Lepidoptera: Pyralidae) and parasitoid Pimpla turionellae L., 1758 (Hymenoptera: Ichneumonidae). The significant increase in hemolytic activity in G. mellonella and P. turionellae hemolymph were observed in 500, 5,000 and 10,000 ppm IAA-treated groups. Phenoloxidase activity in G. mellonella hemolymph significantly decreased with all IAA doses compared to control, however, the reductions in phenoloxidase activity in P. turionellae hemolymph were significant between 500 and 10,000 ppm. Since IAA interacts with both host and parasitoid immunity, it should be used with caution in agricultural areas with high host-parasitoid population.

Keywords

Galleria mellonella, hemolytic activity, Indole-3-acetic acid, phenoloxidase activity, Pimpla turionellae

Supporting Institution

Kocaeli University, Scientific Research Coordination Unit, Türkiye

Project Number

Grant Project No: 2014/068.

Indole-3 asetik asit’in Pimpla turionellae L., 1758 (Hymenoptera: Ichneumonidae) ve Galleria mellonella L., 1758 (Lepidoptera: Pyralidae)’nın konukçu-parazitoit sisteminde fenoloksidaz ve hemolitik aktivitelerine beslenme yoluyla etkileri

Abstract

Gıda ihtiyacının sürekliliği, modern tarımın büyük ölçüde kimyasal girdilere bağımlı hale gelmesine neden olmuştur. Bitki büyüme düzenleyicileri, kemosterilant aktiviteye sahip doğal olarak oluşan veya sentetik bitki kaynaklı kimyasallardır ve zararlı böcekleri baskılama potansiyeline sahiptir. Zararlılarla birlikte, agroekolojik sistemlerdeki parazitoitler gibi hedef olmayan organizmaların, konukçuları aracılığıyla dolaylı temastan veya konukçu ve bitkilerle tritrofik etkileşim yoluyla doğrudan temastan etkilenmesi muhtemeldir. Bu çalışma bitki büyüme düzenleyicisi indol-3-asetik asidin (IAA) depolanmış ürün zararlısı Galleria mellonella L., 1758 (Lepidoptera: Pyralidae) ve parazitoit Pimpla turionellae L., 1758 (Hymenoptera: Ichneumonidae)’nın hemolitik aktivitesi ve fenoloksidaz aktivitesi üzerindeki besinsel etkilerini göstermektedir. Galleria mellonella ve P. turionellae hemolenfinin hemolitik aktivitelerinde istatistiksel olarak önemli artışlar 500, 5000 ve 10000 ppm IAA dozları tatbik edilen gruplarda gözlenmiştir. Galleria mellonella hemolenfinin fenoloksidaz aktivitesi, kontrole kıyasla tüm IAA uygulanan dozlarda önemli ölçüde azalırken, P. turionellae hemolenf fenoloksidaz aktivitesindeki azalmalar 500 ve 10000 ppm arasında anlamlı bulundu. IAA, hem konukçu hem de parazitoit bağışıklığı ile etkileşime girdiğinden, konukçu-parazitoit popülasyonu yüksek olan tarım alanlarında dikkatle kullanılmalıdır.

Keywords

Galleria mellonella, hemolitik aktivite, Indole-3-asetik asit, fenoloksidaz aktivite, Pimpla turionellae

Project Number

Grant Project No: 2014/068.

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