Determination of potential insect vectors and subgroups of aster yellows phytoplasma in the carrot (Daucus carota L.) (Apiaceae) cultivation areas of Ankara and Konya Provinces, Türkiye

Year 2022, Volume: 46 Issue: 4, 385 - 398, 07.01.2023

Filiz Randa Zelyüt , Emre İnak , Emine Demir Özden , Derya Şenal , Filiz Ertunç

https://doi.org/10.16970/entoted.1118787

Abstract

Aster yellows phytoplasma (16Sr-I, AYp) is a widespread plant pathogen affecting a wide range of economically important crops. AYp can be distributed widely via insect vectors and is associated with severe redness and yellowing in carrot leaves. The presence of potential insect vectors of aster yellows phytoplasma was investigated in the Ankara and Konya Provinces, the largest carrot production areas in Türkiye. Forty-five insect samples were collected during the field studies between March and September 2020. Morphological and molecular studies have shown that Neophilaenus campestris (Fallén, 1805) (Hemiptera: Aphrophoridae), Empoasca decipiens Paoli, 1930 (Hemiptera: Cicadellidae) and Psammotettix striatus (L., 1758) (Hemiptera: Cicadellidae) carried 16SrI-F phytoplasma. Psammotettix striatus collected from different locations contained subgroup 16SrI-R, as well. In addition, subgroup 16SrI-B was determined in Cicadula divaricata Ribaut, 1952 (Hemiptera: Cicadellidae) and in a psyllid (Psylloidea: Psyllidae) species. Empoasca sp., Anaceratagallia sp. (Hemiptera: Cicadellidae) and Psammotettix confinis (Dahlbom, 1850) (Hemiptera: Cicadellidae) were determined as potential phytoplasma vectors. Phytoplasma 16Sr rRNA and insect cytochrome oxidase gene nucleotide sequences were used for phylogenetic analysis. The results will contribute to vector-based control of aster yellows phytoplasmas in carrot cultivation areas.

Keywords

Aster yellows, carrot, insect vector, PCR, phylogenetic

Supporting Institution

Bilecik Şeyh Edebali University Scientific Research Projects

Project Number

2017-02.BŞEÜ.06-01

Thanks

This manuscript is a part of the PhD thesis of the first/corresponding author. The authors are grateful to Bilecik Şeyh Edebali University Scientific Research Projects for supporting this project (Code: 2017-02.BŞEÜ.06-01). We would like to thank Dr. Xaiver Foissac for providing a positive control in the molecular detection of phytoplasmas.

Ankara ve Konya (Türkiye) illeri havuç (Daucus carota L.) (Apiaceae) ekim alanlarında aster yellows fitoplazmasının altgruplarının ve potansiyel böcek vektörlerinin belirlenmesi

Abstract

Aster yellows fitoplazma (AYp), ekonomik açıdan önemli farklı tarım ürünlerini etkileyen yaygın bir bitki patojenidir. AYp, böcek vektörleri aracılığıyla geniş alanlara yayılabilir ve havuç yapraklarında şiddetli kızarıklık ve sararma ile ilişkilendirilir. Türkiye'nin en büyük havuç üretim alanları olan Ankara ve Konya illerinde aster yellows fitoplazmasının potansiyel böcek vektörlerinin varlığı araştırılmıştır. Mart-Eylül 2020 tarihleri arasında arazi çalışmalarında 45 böcek örneği toplanmıştır. Morfolojik ve moleküler çalışmalar Neophilaenus campestris (Fallén, 1805) (Hemiptera: Aphrophoridae), Empoasca decipiens Paoli, 1930 (Hemiptera: Cicadellidae) ve Psammotettix striatus (L., 1758) (Hemiptera: Cicadellidae) türlerinin 16SrI-F altgrubu ile bulaşık olduğunu göstermiştir. Bununla birlikte, farklı lokasyonlardan toplanan P. striatus türü ise 16SrI-R altgrubu ile bulaşıktı. Cicadula divaricata Ribaut, 1952 (Hemiptera: Cicadellidae) ve bir psyllid (Psylloidea: Psyllidae) türünde 16SrI-B altgrubu saptanmıştır. Empoasca sp., Anaceratagallia sp. (Hemiptera: Cicadellidae) ve Psammotettix confinis (Dahlbom, 1850) (Hemiptera: Cicadellidae) ise potansiyel fitoplazma vektörleri olarak belirlenmiştir. Elde edilen fitoplazma 16S rRNA ve böcek sitokrom oksidaz genlerinin nükleotit dizileri filogenetik çalışmalarda kullanılmıştır. Bu sonuçlar, havuç ekim alanlarında aster yellows fitoplazmalarının vektör kontrolüne katkıda bulunacaktır.

Keywords

Aster yellows, havuç, böcek vektör, PCR, filogenetik

Project Number

2017-02.BŞEÜ.06-01

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