Biocontrol potential of Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH hybrid strain against the beet webworm, Loxostege sticticalis L., 1761 (Lepidoptera: Pyralidae)

Year 2022, Volume: 46 Issue: 4, 399 - 405, 07.01.2023

Emre Dede , Alperen Kaan Bütüner , Alper Susurluk

https://doi.org/10.16970/entoted.1162125

Cited By: 10

Abstract

With limits on the use of pesticides, biological control has become increasingly important. Consequently, entomopathogenic nematodes (EPN) are now used widely in biological control. EPNs can potentially be used against beet webworm, Loxostege sticticalis L., 1761 (Lepidoptera: Pyralidae), which established in sunflower-growing areas in Türkiye in 2022. Therefore, the hybrid EPN strain, Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH, was assessed for this purpose. The study was conducted in Bursa Uludağ University, Faculty of Agriculture, Plant Protection Department, Nematology Laboratory in 2022. Four nematode doses (2, 5, 10 and 20 IJs) were applied to the last instars of L. sticticalis at three temperatures (20, 25 and 30°C). The highest mortality was 97% with 20 IJs dose nematodes at 30°C. LD50 and LD90 of the nematode were determined at all tested temperatures. The lowest LD50 and LD90 were at 30°C; 4.37 and 11.0 IJs, respectively. These results indicated that the HBN strain has potential for control of L. sticticalis.

Keywords

Biological control, Heterorhabditis bacteriophora, Loxostege sticticalis, sunflower

Supporting Institution

TUBITAK (The Scientific and Technological Research Council of Türkiye)

Project Number

219O370

Thanks

This study was financially supported by the TUBITAK (The Scientific and Technological Research Council of Türkiye), Project number: 219O370. Dr. Tufan Can Ulu is thanked for statistical support.

Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) hibrit HBH ırkının ayçiçeği çayır tırtılı, Loxostege sticticalis L., 1761 (Lepidoptera: Pyralidae)’e karşı biyolojik mücadele potansiyeli

Abstract

Pestisit kullanımının sınırlandırılması ile birlikte buna alternatif olan biyolojik mücadele giderek daha önemli hale gelmiştir. Bu nedenle, 2022 yılında Türkiye'de ayçiçeği tarlalarında ayçiçeği yetiştirilen tarım alanlarında istilaya neden olan çayır tırtılı, Loxostege sticticalis L., 1761 (Lepidoptera: Pyralidae) zararlısına karşı EPN'lerin potansiyel olarak kullanılabileceği düşünülmektedir. Bu çalışmada Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) hibrit ırkı HBH kullanılmıştır. Bu çalışma 2022 yılında Bursa Uludağ Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü, Nematoloji Laboratuvarı’nda yürütülmüştür. Bu çalışmada 4 farklı nematod dozu (2, 5, 10 ve 20 IJs), böceğin son dönem larvası üzerine üç farklı sıcaklıkta (20, 25 ve 30°C) uygulanmıştır. Sonuçlara göre, en yüksek ölüm oranı %97 olarak 20 IJs doz nematod yoğunluğunda 30°C’de elde edilmiştir. Ayrıca, HBH hibrit ırkının LD50 ve LD90 değerleri uygulamada kullanılan tüm sıcaklık değerlerinde belirlenmiştir. En etkili LD50 ve LD90 değeri sırasıyla 4.37 ve 11.0 IJs olarak 30°C’de gözlenmiştir. Sonuçlar, bu HBH ırkın L. sticticalis’ye karşı potansiyel bir ajan olabileceğini göstermiştir.

Keywords

Biyolojik mücadele, Heterorhabditis bacteriophora, Loxostege sticticalis, ayçiçeği

Project Number

219O370

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