Genetic diversity of Steinernema feltiae Filipjev, 1934 (Rhabditida: Steinernematidae) and Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) in potato production areas of Türkiye

Year 2024, Volume: 48 Issue: 2, 229 - 238, 12.07.2024

Ebubekir Yüksel , Arife Gümüş Askar , Alper Susurluk , Refik Bozbuga , Dilek Dinçer , Mustafa Imren

https://doi.org/10.16970/entoted.1475232

Abstract

Entomopathogenic nematodes (EPNs) are one of the most important biological control agents and have proved their biocontrol success against a variety of insect pests. However, limited knowledge exists regarding the genetic structure of various species and populations of EPNs. Thus, this study was conducted to isolate and elucidate the EPN's phylogenetic diversity sourced from potato (Solanum tuberosum L.) (Solanales: Solanaceae) crops in 2020 at Bolu Abant Izzet Baysal University. Through ribosomal DNA sequencing, we investigated genetic variability within and among isolates of Steinernema and Heterorhabditis species. Widespread sampling across Afyonkarahisar, Bolu, İzmir, Sivas, Niğde, Kayseri, and Konya provinces, covering a total area of 795 hectares, led to the recovery of two EPN isolates, constituting 10% of the samples. Molecular characterization involved ribosomal DNA sequencing, which, upon integration with sequences from 41 populations, confirmed the identification of Steinernema feltiae Filipjev, 1934 (Rhabditida: Steinernematidae) and Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae), displaying monophyly in most Steinernema and Heterorhabditis clades, respectively. This survey emphasizes the common occurrence of these EPNs in key potato-growing areas in Türkiye, highlighting their biocontrol potential against arthropod pests of agricultural importance.

Keywords

Beneficial nematodes, genetic variability, ribosomal DNA sequencing

Supporting Institution

Scientific and Technological Research Council of Türkiye (TUBITAK)

Project Number

Grant Project No: 119R025

Thanks

The authors are grateful to the Scientific and Technological Research Council of Türkiye (TUBITAK) with Grant Project No: 119R025 for providing financial support.

Türkiye’de patates üretim alanlarında, Steinernema feltiae Filipjev, 1934 (Rhabditida: Steinernematidae) ve Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) genetik çeşitliliği

Abstract

Entomopatojen nematodlar (EPN) en önemli biyolojik mücadele ajanlarından biridir ve çeşitli böcek zararlılarına karşı biyolojik savaşta başarılarını kanıtlamıştır. Ancak çeşitli türlerin ve EPN popülasyonlarının genetik yapısına ilişkin bilgiler sınırlıdır. Bu nedenle, bu çalışma, patates alanlarından elde edilen EPN'lerin filogenetik çeşitliliğini ortaya koymak ve tel kurtlarının, özellikle Agriotes spp. (Coleoptera: Elateridae) türlerinin mücadelesi için alternatif mücadele yöntemlerini belirlemek amacıyla 2020 yılında Bolu Abant İzzet Baysal Üniversitesi’nde yapılmıştır. Ribozomal DNA dizileme yoluyla, Steinernema ve Heterorhabditis türlerinin izolatları arasındaki genetik farklılıklar araştırılmıştır. Afyonkarahisar, Bolu, İzmir, Sivas, Niğde, Kayseri ve Konya illerini kapsayan geniş bir örnekleme ile, toplamda 795 hektarlık bir alanı kapsayarak, örneklerin %10'unu oluşturan iki EPN izolatının elde edilmiştir. Moleküler karakterizasyon, ribozomal DNA dizileme içermekte ve 41 popülasyonun dizileriyle incelendiğinde, Steinernema feltiae Filipjev, 1934 (Rhabditida: Steinernematidae) ve Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae)’nın varlığını doğrulayarak, çoğu Steinernema ve Heterorhabditis kladlerinde yer almıştır. Bu araştırma, bu EPN'lerin Türkiye'deki önemli patates yetiştirme alanlarında yaygın olarak bulunduğunu göstermekte ve bunların tarımsal öneme sahip arthropod zararlılara karşı biyolojik savaşta potansiyellerini vurgulamaktadır.

Keywords

Faydalı nematodlar, genetik çeşitlilik, ribosomal DNA sekans

Project Number

Grant Project No: 119R025

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