Prodigiosin, Thaumetopoea wilkinsoni (Tams, 1926) (Lepidoptera: Notodontidae)’ya karşı umut verici bir biyolojik kontrol ajanı

Year 2024, Volume: 48 Issue: 3, 343 - 352, 16.10.2024

Muhammed Koç , Duygu Bekircan Eski , İsmail Demir , Ardahan Eski

https://doi.org/10.16970/entoted.1517520

Abstract

Serratia marcescens Bizio (Enterobacteriaceae: Serratia) hidrolitik enzimler ve toksinler üreten entomopatojenik bir bakteridir. Ayrıca, prodigiosin adı verilen çeşitli biyolojik özelliklere sahip bir pigment de üretir. Çalışma, 2023 yılında Bilecik Şeyh Edebali Üniversite’sinde gerçekleştirilmiştir. Bu çalışmada, besiyeri, inkübasyon sıcaklığı ve süresinin S. marcescens Se9’un prodigiosin üretim sürecine etkileri ve farklı çözücülerin ekstraksiyon verimliliği ilk kez ortogonal Taguchi dizi tasarımı kullanılarak optimize edilmiştir. Optimum pigment verimi, 30ºC'de 96 saat boyunca triptik soya sıvı besiyeri ortamında büyütülen bakterilerden metanol ekstraksiyonuyla elde edilmiştir. Belirlenen optimum koşullar altında yapılan validasyon deneyinde prodigiosin pigment verimi 83,4±1,7 mg/L olarak belirlenmiştir. Prodigiosin pigmentinin Thaumetopoea wilkinsoni (Tams, 1926) (Lepidoptera, Notodontidae) larvaları üzerindeki insektisidal potansiyeli ilk kez gösterilmiştir. 1000 ppm pigmente maruz kalan larvalarda ölüm oranı sadece %40 iken, uygulanan konsantrasyonun iki katına çıkarılmasının larva ölümlerinde önemli bir artışa neden olarak %91'e ulaştığı gözlenmiştir. T. wilkinsoni’nin dördüncü larva dönemi için pigmentin LC50 değeri 1192 ppm olarak belirlenmiştir. Çalışma, prodigiosin pigmentinin T. wilkinsoni’nin kontrolü için umut verici bir biyokontrol ajanı olabileceğini göstermiştir.

Keywords

İnsektisidal aktivite, mikrobiyal kontrol, pigment, Serratia, Thaumetopoea wilkinsoni

Prodigiosin, a promising biocontrol agent against Thaumetopoea wilkinsoni (Tams, 1926) (Lepidoptera: Notodontidae)

Abstract

Serratia marcescens Bizio (Enterobacteriaceae: Serratia) is an entomopathogenic bacterium that produces hydrolytic enzymes and toxins. It also produces a pigment with various biological properties called prodigiosin. The study was conducted at Bilecik Seyh Edebali University in 2023. In this study, the effects of medium, incubation temperature and time on the process of prodigiosin production by S. marcescens strain Se9 and the extraction efficiency of different solvents were optimized for the first time using the orthogonal Taguchi array design. The optimal yield of pigment was achieved by methanol extraction from bacteria grown in tyriptic soy broth medium at 30ºC for 96 hours. The yield of prodigiosin pigment was 83.4±1.7 mg/L in the validation experiment conducted under the optimum conditions determined. The insecticidal potential of pigment against the larvae of Thaumetopoea wilkinsoni (Tams, 1926) (Lepidoptera, Notodontidae) was demonstrated for the first time. While the mortality rate in larvae exposed to 1000 ppm of the pigment was only 40%, it was observed that doubling the applied concentration led to a significant increase in larval mortality, reaching 91%. The LC50 value of the pigment for the fourth larval stage of T. wilkinsoni was determined to be 1192 ppm. The study showed that the pigment prodigiosin may be a promising biocontrol agent for the control of T. wilkinsoni.

Keywords

Insecticidal activity, microbial control, pigment, Serratia, Thaumetopoea wilkinsoni

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