Ramalina fraxinea Bazlı Ag NP'lerin Boyutunun Antimikrobiyal Aktiviteler Üzerindeki Etkisinin Değerlendirilmesi

Abstract

Bu çalışmada ilk kez Ramalina fraxinea özütü ile farklı konsantrasyonlarda AgNO3 reaksiyonu sonucunda elde edilen farklı boyutlara sahip Ag NP’lerin karakterizasyonları değerlendirilmiştir. Bu amaçla NP’lerin UV-Vis. analizi ile karakteristik ışık emme noktaları, zeta testi ile yüzey yükleri DLS analizi ile hidrodinamik çapları, SEM analizi ile NP’le- rin morfoloji ve çapları tespit edilmiştir. Ag NP’lerin kristal yapıları XRD haritası ile doğrulanmıştır. Ag NP’nin sentezinde aktif olarak rol oynayan R. fraxinea özütünün fonksiyonel grupları FT-IR analizi ile elde edilen pikler ile ortaya konmuş- tur. Yapılan karakterizasyon testleri sonucunda Ag NP’nin sentezinde kullanılan Ag NO3 konsantrasyonunun artışı ile birlikte (10-3 M-5*10-2 M) AgNP’lerin ortalama çapı 14 nm’den 48 nm’ye artış göstermiştir. Staphylococcus aureus, Esc- herichia coli ve Candida albicans suşlarına karşı Ag NP’lerin antimikrobiyal etkinliği NP’lerin boyutuna bağlı olarak de- ğerlendirilmiştir. Küçük boyuta sahip Ag NP’lerin çalışılan suşlara karşı antimikrobiyal etkisi büyük boyuta sahip AgNP’lere göre oldukça etkiliydi. Sonuç olarak R. fraxinea özütü ile biyolojik olarak ucuz etkili ve çevre dostu yöntemle farklı boyutlarda sentezlenen AgNP’lerin boyuta bağlı olarak antimikrobiyal aktivite sergilediği görülmektedir. Çalışma verilerinin nanoteknoloji ve biyomedikal alan çalışmaları için yol gösterici olacağı düşünülmektedir.

Keywords

• Antimikrobiyal aktivite
• biyolojik sentez
• Ramalina fraxinea
• gümüş nanopartikülleri

Evaluation of the Effect of the Size of Ramalina fraxinea Based Ag NPs on the Antimicrobial Activity

Abstract

For the first time in this study, the effect of nanoparticles (NP) sizes on the antimicrobial activity of Ag NPs of different sizes obtained by the reaction of Ramalina fraxinea(R. fraxinea) extract and silver nitrate (AgNO3) at different concentrations was evaluated. For this purpose, characteristic light absorption points, and charge of the surface were determined by ultraviolet-visible spectroscopy (UV-Vis), and zeta potential, hydrodynamic diameters by dynamic light scattering (DLS) analysis, morphology, and diameters of NPs were determined by scanning electron microscope (SEM) analysis. The crystal structures of Ag NPs were confirmed by the X-ray diffraction (XRD) analysis map. The functional groups of the R. fraxinea extract, which plays an active role in the synthesis of Ag NP, were revealed by the peaks obtained by Fourier transform infrared spectroscopy (FT-IR) analysis. As a result of the characterization tests, the average diameter of Ag NPs increased from 14 nm to 48 nm with the increase in Ag NO3 concentration used in the synthesis of Ag NP (from 10-3 M to 5*10-2 M). The antimicrobial activity of Ag NPs against Staphylococcus aureus, Escherichia coli, and Candida albicans strains was evaluated depending on the size of the NPs. The antimicrobial effect of small-sized Ag NPs against the studied strains was quite effective compared to large-sized Ag NPs. As a result, it is seen that Ag NPs synthesized by using R. fraxinea extract in different sizes with a biologically inexpensive and eco friendly method exhibit antimicrobial activity depending on the size. It is thought that the study data will be a guide for nanotechnology and biomedical field studies.

Keywords

• Antimicrobial activity
• biosynthesis
• Ramalina fraxinea
• silver nanoparticles

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