Investigation of the Presence of Stem Cells in Rat Uterus Tissue in Postnatal Development Periods

Abstract

Uterine tissue is an organ with a high proliferation capacity where regeneration, differentiation and shedding are seen. Although this tissue is regenerated every month, the source of it hasn’t been determined yet. Studies are carried out on stem cells being a source that can provide regeneration of the endometrium and the markers expressed by these cells. The purpose of this study was to examine the immunreactivity of CD9 and CD13 expressed by essential population of uterus and CD34, marker of hematopoietic stem cells, at various stages of the postnatal developing pro- cess. In this study, was used 42 female Wistar-albino rats split into six groups; Group I; newborn (2-days), Group II; pubertal (38-days), and Group III: fertile group (12 weeks), by identifying the stages of the estrous cycle in rats a) pro- estrus, b) estrus, c) metestrus, d) diestrus. The expression of CD9, CD13, and CD34 in uterine tissues excised from rats was investigated. In the groups of 38-days and 12-weeks, CD34-expressing cells were present in the stroma of the endometrium next to the myometrium, but such cells were absent in the 2-days group. All groups' uterine epithelium displayed CD9 expression, except for group 2-days. Endometrial stromal cells that expressed CD13 showed only little immureactivity in groups 2 and 38-days, while CD13 expression is noticeable in group 12-weeks. It was thought that the uterine endometrium could be regenerated in puberty and adulthood with the contribution of bone marrow-derived stem cells and uterine-derived epithelial and stromal cells.

Keywords

• Endometrium
• immunohistochemistry
• smear

Doğum Sonrası Gelişim Dönemlerindeki Sıçan Uterus Dokusunda Kök Hücre Varlığının Araştırılması

Abstract

Uterus dokusu yenilenme, farklılaşma ve dökülmenin görüldüğü yüksek proliferasyon kapasitesine sahip olan bir organdır. Bu dokuda yenilenme her ay olmasına rağmen, yenilenmenin kaynağı halen belirlenememiştir. Kök hücrelerin endometriyumun rejenerasyonunu sağlayabilen bir kaynak olduğu ve bu hücrelerin eksprese ettiği belirteçler ile ilgili çalışmalar yapılmaktadır. Bu çalışmanın amacı, uterusun temel popülasyonu tarafından eksprese edilen CD9 ve CD13'ün ve hematopoetik kök hücrelerin belirteci olan CD34'ün doğum sonrası gelişim sürecinin çeşitli aşamalarında immünreaktivitesini incelemekti. Bu çalışmada 42 adet dişi Wistar albino rat altı gruba ayrıldı; Grup I yenidoğan (2 günlük), Grup II pubertal (38 günlük) ve geri kalan gruplar, Grup III: fertil grup (12 hafta), sıçanlarda östrus döngüsünün evrelerini belirleyerek a) proöstrus, b) östrus, c) metestrus, d) diestrus. Deney sonunda, sıçanlardan alınan uterus dokularında CD9, CD13 ve CD34 ekspresyonları araştırıldı. 38 gün ve 12 haftalık postnatal gruplarda, miyometriyumun yanındaki endometrium stromasında CD34 eksprese eden hücreler mevcutken, postnatal 2 günlük grupta böyle hücreler bulunmamaktaydı. CD9 ekspresyonun da uterus epitelinde 2 günlük grup dışındaki tüm gruplarda gözlendi. Endometriyum stromasında eksprese edilen CD13 ise 2 günlük ve 38 günlük sıçanlarda zayıf olmasına rağmen, 12 haftalık sıçanlarda belirgin CD13 ekspresyonu olduğu belirlendi. Uterus endometriyumunun puberte ve erişkin dönemlerde, kemik iliği kaynaklı kök hücrelerin ve uterus kaynaklı epitelyal ve stromal hücrelerin katkısı ile yenilenebileceği düşünülmüştür.

Keywords

• Endometriyum
• immünohistokimya
• smear

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