Karahindiba (Taraxacum officinale) Ekstraktı Uygulanan Diyabetik Ratların Testis Dokusunda Histolojik ve İmmunohistokimyasal İncelemeler

Abstract

Bu çalışma deneysel diyabet oluşturulan ratlarda Karahindiba (Taraxacum officinale) ekstraktı uygulamasının testis dokusunda meydana getirdiği değişimleri ve enzimatik antioksidanlardan SOD-2 salınımı üzerindeki etkilerini immuno-histokimyasal yöntemlerle ile değerlendirmek amacı ile yapıldı. Diyabet grubu testis kesitlerinde seminifer tübül yapısında yer yer dejeneratif değişiklikler izlendi. Bazal membran ile tübül epiteli arasında ayrışmalar, tübül lümenine doğru spermatogenik hücrelerin yapısında ve yerleşiminde bozulmalar, bazal membran ve tunika albugineada kalınlaşmalar, sertoli hücrelerinde şekil değişikliği ve leyding hücre çekirdeğinde küçülmeler olduğu belirlendi. Diyabet grubunda spermatogonyumlar ve primer spermatositlerde immun pozitiflik gözlenmezken, spermatogenik seriye ait diğer hücrelerde zayıf, diyabet+karahindiba gurubunda orta derecede SOD-2 immunoreaktivitesi gözlendi. Sonuç olarak kronik metabolik bir hastalık olan diyabet hastalığında testis dokusunda histopatolojik değişimlerin meydana geldiği ve SOD-2 immunoreaktivitesinin azaldığı belirlendi. Karahindiba uygulamasının azalmış olan SOD-2 immuno-reaktivitesi üzerinde olumlu etkileri olabileceği kanısına varıldı.

Keywords

• Diyabet
• karahindiba
• SOD-2
• testis

Histological and Immunohistochemical Examination in Testicular Tissue of Diabetic Rats Treated with Dandelion (Taraxacum officinale) Extract

Abstract

The aim of this study was to evaluate the changes induced in testicular tissue by administration of dandelion (Taraxacum officinale) extract and its effects on the release of enzymatic antioxidants SOD-2 by immunohistochemical methods in rats with experimental diabetes. Degenerative changes in the seminiferous tubule structure were observed in the testicular sections of the diabetes group. Separations between the basement membrane and the seminiferous epithelium, disruptions in the structure and localization of spermatogenic cells toward the lumen of the seminiferous tubules, thickening of the basement membrane and tunica albuginea, changes in the shape of Sertoli cells, and shrinkage of the nucleus of Leydig cells were observed. While no immunopositivity was observed in spermatogonium and primary spermatocytes in the diabetes group, weak SOD-2 immunoreactivity was observed in other cells of the spermatogenic series and moderate SOD-2 immunoreactivity was observed in the diabetes+dandelion group. In conclu-sion, it was found that histopathological changes occurred in the testicular tissue and SOD-2 immunoreactivity decreased in diabetes, a chronic metabolic disease. It was concluded that dandelion administration may have beneficial effects on decreased SOD-2 immunoreactivity.

Keywords

• Dandelion
• diabetes
• SOD-2
• testicular

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