Şap Hastalıklı Besi Sığırlarında Pratik Kardiyak Troponin I Kit Sonuçları ve Kardiyak Troponin I Düzeyleri

Abstract

 Bu çalışmanın amacı, immünokromatografik test kitleri ile şap hastalığı (FMD) bulunan besi sığırlarında cTn-I’nın belirlenmesi ve miyokard dejenerasyonlarında söz konusu parametrenin düzeylerini belirlemekti. Bu amaçla akut FMD’li (I. Grup) ortalama 12.4±0.5 aylık yaşta 69 adet ve klinik olarak sağlıklı (II. Grup) ortalama 10.4±0.6 aylık yaşta 20 adet sığır kullanıldı. Her iki grupta da kreatin kinaz-MB (CK-MB) ve laktat dehidrogenaz (LDH ) aktiviteleri, cTn-I düzeyleri ve cTn-I varlığı belirlendi. FMD’li sığırlarda ortalama CK-MB ve LDH aktiviteleri, cTn-I düzeyi sırasıyla ortalama 795.1±70.8 U/L, 1305.9±51.4 U/L ve 3.6±0.3 ng/ml olarak bulundu. Aynı parametreler II.Grupta sırasıyla ortalama 306±20.5 U/L, 1084.7±43.2 U/L ve 1.1±0.02 ng/ml olduğu belirlendi. Bu parametreler I. Grupta, kontrol grubuna göre istatistiksel olarak yüksek bulundu (p<0.05). Pratik cTn-I kitlerinin kalp kası dejenerasyonunun tanısında duyarlılık ve seçicilik oranları sırasıyla %100 ve %76.92 olarak bulundu. FMD’li sığırlardan yalnızca beşinde kardiyak Tn-I testleri pozitif sonuç verdi. Ancak bu pratik testler sağlıklı sığırlardan toplanan kan örneklerinde negatif idi. Makroskobik bulgular, serum cTn-I düzeyleri ve pozitif cTn-I sonuçları Şap hastalığına bağlı miyokard dejenerasyonu olan vakalarla uyumlu bulundu. Sonuç olarak, hem kantitatif cTn-I analizleri hem de kalitatif cTn-I varlığı FMD’li sığırlarda ölüm oranının belirlenmesi için duyarlı ve spesifik bir belirleyici olabileceği ve sığırlarda gelişebilecek kalp kası hastalıklarını belirlemek için kullanılabileceği gösterilmiştir.

Keywords

• CK-MB,cTn-I,LDH,sığır,şap hastalığı

Practice Cardiac Troponin I Kit Expressions and Concentrations of Cardiac Troponin I in Beef Cattle with Foot and Mouth Disease*

Abstract

 The aim of this study was to determine the expression of cTn-I with immunochromatographic strip kits, the concentrations of serum cTn-I in myocardial degeneration due to foot and mouth disease (FMD) with these parameters in beef cattle. For this purpose sixtynine cattle with acute FMD (Group I), mean age 12.4±0.5 months old and 20 clinically healthy cattle (Group II), mean age 10.4±0.6 months old were used. Creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH) activities, cTn-I concentrations and expression of cTn-I were determined in both groups. Mean CK-MB, LDH activities and cTn-I concentration were found as 795.1±70.8 U/L, 1305.9±51.4 U/L and 3.6±0.3 ng/ml respectively in cattle with FMD. The mean of the same parameters were 306±20.5 U/L, 1084.7±43.2 U/L and 1.1±0.02 ng/ml in Group II, respectively. These parameters in Group I were statistically higher than those of control group (p<0.05). The sensitivity and specificity of cTn-I kits for the diagnosis myocardial degeneration were 100% and 76.9%, respectively. Cardiac Tn-I tests were positive in the only five cattle with FMD, but these tests were negative in blood samples collected from healthy cattle. Macroscobic findings, serum cTn-I concentrations and positive cTn-I results were correlated with FMD cases suffering from myocardial degeneration. In conclusion, both quantitative cTn-I analysess and qualitative cTn-I expressions may be sensitive and specific marker for the determination of mortality rate in cattle with FMD, and can be used to predict the future cardiac disorders in cattle.

Keywords

• Cattle,CK-MB,cTn-I,FMD,LDH

References

1. 1. Al Shami SA. Studies on Normal Haematological and Biochemical Parameters of Hassawi cattle Breed in Saudia Arabia. Pak J Biol Sci 2003; 6 (14): 1241-2.
2. 2. Azzazy HME, Christenson RH. Cardiac markers of acute coronary syndrome: Is there a case for point-of-care testing. Clin Biochem 2002; 35(1): 13-27.
3. 3. Bademkiran S, Yokus B, Icen H, Cakir DU, Kurt D. Assesment of Serum Mineral and Certain Biochemical Variables in Self-Sucking Dairy Cows. JAVA 2008; 7 (24): 717-22.
4. 4. Barnett PV, Cox SJ. The role of small ruminants in the epidemiology and transmission of Foot-and-Mouth Disease. Vet J 1999: 158(1): 6-13.
5. 5. Bertsch T, Bleuel H, Aufenanger J, Rebel W. Comparison of cardiac troponin-T and cardiac troponin-I concentrations in peripheral blood during orciprenaline induced tachycardia in rats. Exp Toxicol Pathol 1997; 49 (6): 467-8.
6. 6. Boccara G, Pouzeratte Y, Troncin R, Bonardet A, Boularan AM, Colson P, Mann C. The risk of cardiac injury during laparoscopic fundoplication. Cardiac troponin I and ECG study. Acta Anaesthesiol Scand 2000; 44(4): 398-402.
7. 7. Brandt RR, Filzmaier K, Hanrath P. Circulating cardiac troponin I in acute pericarditis. Am J Cardiol 2001; 87(11): 1326-8.
8. 8. Buczinski S, Bélanger AM. Bovine tricuspid endocarditis as a cause of increased serum concentration of cardiac troponins. Can Vet J 2010; 51(12): 195-7.

9. 9. Buczinski S, Rezakhani A, Boerboom D. Heart disease in cattle: Diagnosis, therapeutic approaches and prognosis. Vet J 2010; 184(3): 258-63.
10. 10. Cardinet GH. Skeletal muscle function. K. Kaneko, eds. In Clinical Biochemistry of Domestic Animals. Fifth Edition. San Diego: Academic Press, 1997a; pp. 410-4.
11. 11. Cardinet GH. Skeletal muscle function. K. Kaneko, eds. In Clinical Biochemistry of Domestic Animals. Fifth Edition. San Diego: Academic Press, 1997b; pp. 424-9.
12. 12. Christenson RH, Apple FS, Morgan DL, Alonsozona GL, Mascotti K, Olson M, McCormack RT, Wians FH Jr, Keffer JH, Duh SH. Cardiac troponin-I measurement with the Access immunoassay system: analytical and clinical performance characteristics. Clin Chem 1998; 44(1): 52-60.
13. 13. Doornenbal H, Tong AKW, Murray NL. Reference Values of Blood Parameters in Beef Cattle of Different Ages and Stages of Lactation. Can J Vet Res 1988; 52(1): 99-105.
14. 14. Gunes V, Atalan G, Citil M, Erdogan HM. Use of cardiac troponin kits for the qualitative determination of myocardial cell damage due to traumatic reticuloperitonitis in cattle. Vet Rec 2008; 162(16): 514-7.
15. 15. Gunes V, Erdogan HM, Citil M, Ozcan K. Assay of cardiac troponins in the diagnosis of myocardial degeneration due to foot-and-mouth disease in a calf. Vet Rec 2005; 156(22): 714-5.
16. 16. Imazio M, Demichelis B, Cecchi E, Belli R, Ghisio A, Bobbio M, Trinchero R. Cardiac troponin I in acute pericarditis. J Am Coll Cardiol 2003; 42(12): 2144-8.
17. 17. Jesty SA, Sweeney RA, Dolente BA, Reef BA. Idiopathic and cardiac tamponade in two cows. J Am Vet Med Assoc 2005; 226(9): 1555-8.
18. 18. Jurlander B, Clemensen P, Wagner GS, Grande P. Very early diagnosis and risk stratification of patients admitted with suspected acute myocardial infarction by the combined evaluation of a single serum value of cardiac troponin-T, myoglobin, and creatine kinase MB. Eur Heart J 2000; 21(5): 382-9.
19. 19. Karapinar T, Dabak DO, Kuloğlu T, Bulut H. High cardiac troponin I plasma concentration in a calf with myocarditis. Can Vet J 2010; 51: 397-9.
20. 20. M-Bardorff M, Hallermayer K, Schro A, Ebert C. Improved troponin T ELISA specific for cardiac troponin T isoform: assay development and analytical and clinical validation. Clin Chem 1999; 43(3): 458-66.
21. 21. Mellanby RJ, Henry JP, Cash R, Ricketts SW, Bexiga R, Truyers I, Mellor DJ. Serum cardiac troponin I concentrations in cattle with cardiac and noncardiac disorders. J Vet Intern Med 2009; 23(4): 926-30.
22. 22. Mellanby RJ, Henry JP, Cash R, Ricketts SW, Dias Bexiga JR, Mellor DJ. Serum cardiac troponin I concentrations in cattle with pericarditis. Vet Rec 2007; 161(13): 454-5.
23. 23. Mezencio JMS, Babcock GD, Kramer E, Brown F. Evidence for the pesistence of Foot-and-Mouth Disease virus in pigs. Vet J 1999; 157(3): 213-7.
24. 24. O’Brien PJ. Cardiac troponin is the most effective translational safety biomarker for myocardial injury in cardiotoxicity. Toxicol 2008; 245(3): 206-18.
25. 25. O’Brien PJ, Dameron GW, Beck ML. Differantial reactivity of cardiac and skeletal muscle from various species in two generations of cardiac troponin-T immunoassays. Res Vet Sci 1998; 65(2): 135-7.
26. 26. O’Brien PJ, Landt Y, Ladenson JH. Differential reactivity of cardiac and skeletal muscle from various species in a cardiac troponin I immunoassay. Clin Chem 1997; 43(12): 2333-8.
27. 27. Oyama MA, Sisson DD. Cardiac troponin-I concentration in dogs with cardiac disease. J Vet Intern Med 2004; 18(6): 831-9.
28. 28. Panteghini M. Present issues in the determination of troponins and other markers of cardiac damage. Clin Biochem 2000; 33(3): 161-6.
29. 29. Reef VB, Mcguirk SM. Diseases of the cardiovascular system. BP Smith. Eds. In Large Animal Internal Medicine. Third Edition. St. Louis: Mosby, 2002; pp. 443-78.
30. 30. Smith BP. Large Animal Internal Medicine. Third Edition. Philadelphia/USA: Mosby-year book, 2002; p. 718.
31. 31. Tunca R, Erdogan HM, Sonmez M, Citil M, Devrim AK, Erginsoy S, Uzlu E. Evaluation of cardiac troponin I and inducible nitric oxide synthase expressions in lambs with White Muscle Disease. Turk J Vet Anim Sci 2009; 33(1): 53-9.
32. 32. Tunca R, Sozmen M, Erdogan HM, Citil M, Uzlu E, Ozen H, Gokce E. Determination of cardiac troponin I in the blood and heart of calves with foot-and-mouth disease. J Vet Diagn Invest 2008; 20(5): 598-605.
33. 33. Undhad VV, Fefar DT, Jivani BM, Gupta H, Ghodasara DJ, Joshi BP, Prajapati KS. Cardiac troponin: an emerging cardiac biomarker in animal health. Vet World 2012; 5(8): 508-11.
34. 34. Utlu N, Yucel O, Kaya N. Farklı Irk Sığırlarda bazı Serum Enzim Aktiviteleri. Kafkas Univ Vet Fak Derg 2005; 11(1): 39-41.
35. 35. Van Vleet JF, Ferrans JV. Myocardial Disease of Animals. Am J Pathol 1986; 124(1): 98-178.
36. 36. Varga A, Angelos JA, Graham TW, Chigerwe M. Preliminary Investigation of Cardiac Troponin I Concentration in Cows with Common Production Diseases. J Vet Intern Med 2013; 27(6): 1613-21.
37. 37. Varga A, Schober KE, Walker WL, Lakritz J, Michael RD. Validation of a commercially available immunoassay for the measurement of bovine cardiac troponin I. J Vet Intern Med 2009; 23(2): 359-65.
38. 38. Yang PC, Chu RM, Chung WB, Sung HT. Epidemiological characteristic and financial costs of the 1997 foot and-mouth disease epidemic in Taiwan. Vet Rec 1999; 145(25): 731-4.