Kayseri İlinde BLAD Prevalansı ile Taşıyıcı ve Taşıyıcı Olmayan Holştayn İneklerinde Bazı Üretim Parametrelerinin Karşılaştırılması

Abstract

Bu çalışmada, Kayseri ilinde yetiştirilen tesadüfî olarak seçilen 262 Holştayn inek, polimeraz zincir reaksiyonurestiriksiyon fragment uzunluk polimorfizmi (PCR-RFLP) ile sığır lökosit bağlanma yetmezliği (BLAD) yönünden incelenmiş ve BLAD-taşıyıcısı (BL) ve BLAD-taşıyıcısı olmayan (TL) inekler ilkine buzağılama yaşı, buzağılama aralığı ve 305 günlük süt verimi gibi bazı üretim parametreleri bakımından karşılaştırılmıştır. BLAD taşıyıcı ineklerin prevalansı % 4.58 olarak bulunmuştur. BLAD taşıyıcı ve taşıyıcı olmayan inekler arasında ilkine buzağılama yaşı ve buzağılama aralığı parametreleri yönünden herhangi bir fark bulunmamıştır (p>0.05). Ancak, 305 günlük süt verimi BLAD taşıyıcı ineklerde daha yüksek bulunmuştur (p<0.001). Bu nedenle, Holştayn ineklerde bu hastalığın yayılmasını engellemek için BLAD-taşıyıcılarının belirlenmesi ve çiftleştirmelerin buna göre planlanması büyük önem taşımaktadır. 

Keywords

• BLAD,Holştayn,Kayseri,PCR-RFLP

The Prevalence of BLAD and Comparison of Some Production Parameters in Carrier and Non-Carrier Holstein Cows in Kayseri Province, Turkey

Abstract

In this study, randomly selected 262 Holstein cows reared in Kayseri province of Turkey were screened with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for bovine leukocyte adhesion deficiency (BLAD) and some production parameters such as age at first calving, calving interval and 305-day milk production of BLAD-carrier (BL) and BLAD non-carrier (TL) cows were compared. As a result, the prevalence of BL cows was found as 4.58%. There was not any difference between BL and TL cows for age at first calving and calving interval parameters (p>0.05). However, 305-day milk production of BL cows was higher than that of TL cows (p<0.001). Thus, the detection of BLAD carriers and properly planned mating are of great importance for preventing the spread of the disease in Holstein cows.

Keywords

• BLAD,Holstein,Kayseri,PCR-RFLP

References

1. 1. Akyüz B, Ertuğrul O. Detection of bovine leukocyte adhesion deficiency (BLAD) in Turkish native and Holstein cattle. Acta Vet Hung 2006; 54: 173-8.
2. 2. Akyüz B, Ertuğrul O, Ağaoğlu ÖK. Detection of bovine leukocyte adhesion deficiency (BLAD) allele in Holstein cows reared in Kayseri vicinity. Kafkas Univ Vet Fak Derg 2010; 16(2): 519-21.
3. 3. Citek J. Rehout V. Hajkova J. Pavkova J. Monitoring of the genetic health of cattle in the Czech Republic. Vet Med-Czech 2006; 51: 333-9.
4. 4. Czarnik U, Zabolewicz T, Galiñski M, Pareek CS, Walawski K. Silent point mutation polymorphism of the bovine CD18 encoding gene. J Appl Genet 2004; 45(1): 73-6.
5. 5. Distl O. The use of molecular genetics in eliminating of inherited anomalies in cattle. Arch Tierzucht 2005; 48(3): 209-18.
6. 6. Dohy J, Jánosa A, Vági J. Evaluation of BLAD carrier and non BLAD carrier top bulls. 47th Ann Meet EAAP, Lillehammer, Norway, 1996, Proc. pp. 307.
7. 7. Fésüs L, Zsolnai A, Anton I, Bárány I, Bozó S. BLAD genotypes and cow production traits in Hungarian Holsteins. J Anim Breed Genet 1999; 116: 169-74.
8. 8. Gerardi AS. Bovine leukocyte adhesion deficiency: A brief overview of a modern disease and its implications. Acta Vet Hung 1996; 44: 1-8.

9. 9. Jánosa A, Dohy J, Comparison of BLAD carrier and non BLAD carrier top bulls. J Anim Sci 1997; 75(Suppl. 1): 152.
10. 10. Jánosa Á, Baranyai B, Dohy J. Comparison of milk production of the progeny of BLAD-carrier and healthy Holstein bulls in Hungary. Acta Vet Hung 1999; 47: 283-9.
11. 11. Jørgensen CB, Agerholm JS, Pedersen J. Thomsen PD. Bovine leukocyte adhesion deficiency in Danish Holstein Friesian cattle. I. PCR screening and allele frequency estimation. Acta Vet Scand 1993; 34: 231-6.
12. 12. Kriegesmann B, Jansen S, Baumgartner BG, Brenig B. Partial genomic structure of the bovine CD18 gene and the refinement of test for bovine leukocyte adhesion deficiency. J Dairy Sci 1997; 80: 2547-9.
13. 13-Li J, Wang H, Zhang Y, Hou M, Zhong J, Zhang Y. Identification of BLAD and citrullinemia carriers in Chinese Holstein cattle. Anim Sci Pap Rep 2011; 29(1): 37-42.
14. 14. Meydan H, Yildiz MA, Agerholm JS. Screening for bovine leukocyte adhesion deficiency, deficiency of uridine monophosphate synthase, complex vertebral malformation, bovine citrullinaemia, and factor XI deficiency in Holstein cows reared in Turkey. Acta Vet Scand 2010; 52: 56.
15. 15. Nagahata H. Bovine leukocyte adhesion deficiency (BLAD): A review. J Vet Med Sci 2004; 66: 1475-82.
16. 16. Nasreen F, Malik NA, Riaz MN, Qureshi JA. Detection and screening of bovine leukocyte adhesion deficiency in Pakistan using molecular methods. Hereditas 2009; 146: 74-8.
17. 17. Norouzy A, Nassiry MR, Shahrody FE, Javadmanesh A, Mohammad Abadi MR, Sulimova GE. Identification of bovine leucocyte adhesion deficiency (BLAD) carriers in Holstein and brown Swiss AI bulls in Iran. Russ J Genet 2005; 41: 1697-1701.
18. 18. OMIA. http://omia.angis.org.au/home/; Erişim tarihi:03.10.2013
19. 19. Önal AR, Özder M. Edirne ili damızlık sığır yetiştiricileri birliğine üye işletmelerin yapısal özellikleri. Tekirdağ Ziraat Fakültesi Dergisi 2008; 5(2): 197-203.
20. 20. Öner Y. Keskin A. Elmacı C. Identification of BLAD, DUMPS, citrullinaemia and factor XI deficiency in Holstein cattle in Turkey. Asian J Anim Vet Adv 2010; 5: 60-5.
21. 21. Patel RK, Singh KM, Soni KJ, Chauhan JB, Krothapalli RS, Sambasiva R. Low incidence of bovine leukocyte adhesion deficiency (BLAD) carriers in Indian cattle and buffalo breeds. J Appl Genet 2007; 48(2): 153-5.
22. 22. Poli MA, Dewey R, Semorile L, Lozano ME, Albariño CG, Romanowski V, Grau O. PCR screening for carriers of bovine leukocyte adhesion deficiency (BLAD) and uridine monophosphate synthetase (DUMPS) in Argentine Holstein cattle. J Vet Med A 1996; 43: 163-8.
23. 23. Powell RL, Norman HD, Cowan CM. Relationship of bovine leukocyte adhesion deficiency with genetic merit for performance traits. J Dairy Sci 1996; 79: 895-9.
24. 24. Ribeiro LA, Baron EE, Martinez ML, Coutinho LL. PCR screening and allele frequency estimation of bovine leukocyte adhesion deficiency in Holstein and Gir cattle in Brazil. Genet Mol Biol 2000; 23(4): 831-4.
25. 25-Riojas-Valdes VM, Carballo-Garcia B, Rodriuez-Tovar LE, Garza-Zermeno MV, Ramirez-Romero R, Zarate-Ramos J, Avalos-Ramires R, Davalos-Aranda G. Absence of bovine leukocyte adhesion deficiency (BLAD) in Holstein cattle from Mexico. J Anim Vet Adv 2009; 8(9):1870-2.
26. 26. Vătăşescu-Balcan RA, Manea MA, Georgescu SE, Dinischiotu A, Tesio CD, Costache M. Evidence of single point mutation inducing BLAD disease in Romanian Holstein-derived cattle breed. Biotechnology in Animal Husbandry 2007; 23(5-6): 375-81.