Mycobacterium bovis ile Enfekte Sığırlarda Akut Faz Protein ve Bazı Biyokimyasal Parametre Düzeyleri

Abstract

Bu çalışmanın amacı Mycobacterium bovis ile enfekte sığırlarda akut faz proteinler (AFP) ve bazı biyokimyasal parametrelerin düzeyinin belirlenmesidir. Çalışmada biyokimyasal parametreleri araştırmak için ELISA test sonuçlarına göre 25 adet M.bovis ile enfekte ve 25 adet sağlıklı sığır kullanıldı. Hayvanların V. jugularis’inden kan örnekleri antiko-agulansız tüplere alındı. Haptoglobin, serum amiloid A (SAA), seruloplazmin, aspartat amino transferaz (AST), alkalen fosfataz (ALP), gama-glutamil transferaz (GGT), üre, kreatinin ve demir (Fe) düzeyleri kolorimetrik olarak tayin edildi. Yapılan analizler sonucunda M.bovis ile enfekte sığırlar ile kontrol grubundaki hayvanlar karşılaştırıldığında AFP’lerden haptoglobin, SAA ve seruloplazmin; biyokimyasal parametrelerden ise AST, ALP, GGT, üre ve kreatinin düzeylerinin kontrol grubuna göre istatistiksel olarak anlamlı derecede yükseldiği, serum Fe düzeyinin ise düştüğü belirlendi. Sonuç olarak M.bovis ile enfekte sığırlarda akut faz yanıt oluştuğu ve bunun sonucu olarak da AFP’lerin arttığı, karaciğer fonksiyonlarının bozulduğu belirlenmiştir.

Keywords

• Akut faz proteinler,biyokimyasal parametreler,Mycobacterium bovis,sığır

Levels of Acute Phase Protein and Some Biochemical Parameter in Cattle Infected with Mycobacterium Bovis

Abstract

In this study, it was aimed to determine the levels of acute phase proteins (APP) and some biochemical parameters in cattle infected with Mycobacterium bovis. Twenty-five M. bovis infected and twenty-five antibody-negative healthy bovine sera were used according to ELISA test results to investigate the biochemical parameters. Blood samples obtained from Jugular veins of animals were collected into plain tubes. Haptoglobin, serum amyloid A (SAA), ceruloplasmin, aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), urea, creatinine and iron (Fe) levels were measured colorimetrically. Compared with the control group, cattle with tuberculosis were found have statistically significant increased levels of APP such as serum haptoglobin, SAA and ceruloplasmin and levels of biochemical parameters such as AST, ALP, GGT, urea and creatinine while decreased levels of serum Fe. It was determined that acute phase response occurred in cattle infected with M. bovis which led to increase APP and impairment in the normal functions of the liver.

Keywords

• Acute phase proteins,biochemical parameters,cattle,Mycobacterium bovis

References

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