Türkiye’de Yetiştirilen Kıl ve Halep Keçilerinde Beta-Laktoglobulin (β-LG)/ SacII Polimorfizminin Belirlenmesi

Abstract

Bu çalışmada Türkiye yerli keçi ırklarından Kıl ve Halep (Damascus) ırklarında beta-laktoglobulin (β-LG) gen polimorfizminin belirlenmesi amaçlanmıştır. Çalışmada toplam 269 baş keçide β-LG genotipleri SacII enzim kesimi ile belirlenmiştir. Örneklere ait PCR ürünlerinin SacII enzimi ile kesimleri sonucunda A ve B olarak isimlendirilen iki allel belirlenmiştir. İncelenen ırklarda AA genotipinin frekansı Kıl keçilerinde 0.721, Halep keçilerinde ise 0.948 olarak belir-lenmiştir. AB genotipinin frekansı ise Kıl keçilerinde 0.279, Halep keçilerinde ise 0.052 olarak belirlenmiştir. İncelenen her iki keçi ırkında da BB genotipine rastlanılmamıştır. Çalışma sonunda Kıl keçilerinde Hardy-Weinberg (H-W) dengesinden sapma gözlenmişken (P<0.05), Halep ırkının H-W dengesinde olduğu belirlenmiştir. Bu çalışma ile Halep keçilerinde β-LG-SacII polimorfizmi ilk kez belirlenmiştir.

Keywords

• Beta-laktoglobulin geni,keçi,polimorfizm,RFLP

Detection of Beta-Laktoglobulin (β-LG)/ SacII Gene Polymorphism in Hair and Damascus Goat Breeds in Turkey

Abstract

This disquisition was aim to detect the allele structures of beta-lactoglobulin (β-LG) gene in Hair (172) and Halep (Damascus) (97) breeds that have been grown in Turkey. A total of 269 goats were analized for the β-LG-SacII polymorphism by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). In the exam-ined breeds, digestion the PCR products with SacII restriction enzyme show two alleles (A and B) and two genotypes (AA and AB). The genotype BB was not located on both of Hair and Halep variety. Genotypic frequencies of allele B were 0.721 and 0.948 for AA and 0.279 and 0.052 for AB in order. Deflection from Hardy-Weinberg equilibrium was tracked in the Hair goat breed (P<0.05) while deflection from Hardy-Weinberg equilibrium was not tracked in the Halep goats. Hereby, this work provided data on the β-LG polymorphism of two Turkish goat variety. Moreover, this work reported the presence of a genetic β-LG- SacII polymorphism in Halep goat variety in Turkey for the first time.

Keywords

• Beta-lactoglobulin gene,goat,polymorphism,RFLP

References

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