Rat Epididimal Spermasının Kısa Süreli Saklanmasında Farklı Sıcaklık Dereceleri ve Karnitinin Etkileri

Year 2019, , 30 - 37, 25.03.2019

Serpil Sarıözkan

https://doi.org/10.32707/ercivet.538016

Abstract

Bu çalışmanın amacı, kısa süreli saklanan
(0., 6., 12., 24. ve 48. saat) rat epididimal spermasında farklı saklama
derecelerinin (4 ^oC ve 37 ^oC) ve karnitinin spermatolojik
parametreler üzerine etkisini araştırmaktır. Araştırmada sperma donörü
olarak 10 adet 8-10 haftalık yaş ve 200-250 g ağırlıklarında Wistar-Albino erişkin rat
kullanıldı. Epididimisler anestezi altındaki ratlardan cerrahi teknikle alındı.
Epididimal sperma numuneleri önce 4 ayrı gruba ayrıldı, numuneler Tris (T) ve
Tris + 2 mM  karnitin (TC) ile
sulandırıldı. Ardından sulandırılan sperma numuneleri 4 ^oC (T - TC)
ve 37 ^oC (T - TC) de 48 saat süreyle kısa süreli saklandı. Saklama
süresinin ardından motilite ve anormal spermatozoa oranı analiz edildi.
Uygulama 10 kez tekrar edildi. Çalışmanın sonucunda, tüm deneysel gruplarda 48.
saat sonunda anormal spermatozoa oranında artış ve motilite oranında düşme
saptandı. Her iki sıcaklık derecesinde motilite ve spermatozoa morfolojik
yapısının karnitin katılan gruplarda (TC) daha iyi korunduğu belirlendi. Sonuç
olarak, 4 ^oC deki motilite oranı 37 ^oC ye kıyasla daha
yüksek ve anormal spermatozoa oranı ise daha düşük bulundu. 

Keywords

Rat, epididimal sperma, karnitin, kısa süreli saklama, spermatolojik parametreler

References

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