Investigation of the Effects of Boric Acid on Preventing Lung Damage in the End of the Lower Extremity Ischemia Reperfusion in Rats

Abstract

In this study, the biochemical and histopathological changes in the lungs due to lower extremity ischemia-reperfusion injury and protective effect of boric acid to prevent them were investigated. The rats were divided into three groups as Group 1 (Sham group, n=9), Group 2 (Ischemia-reperfusion group, I/R, n=9) and Group 3 (Ischemia-reperfusion, (I/R) + boric acid (BA), n=9). Anaesthesia was applied to the first group of rats without any ischemia-reperfusion process. Following a two-hour ischemia, two hours of reperfusion, with the help of left lower extremities tourniquet, were applied to the second group. To the third group, 200 mg/kg (i.p.) boric acid application was performed 10 minutes before the ischemia was initiated and two hours of ischemia and two hours of reperfusion application were carried out. Some antioxidant enzymes in lung tissue (SOD, GSH-Px, CAT) were analyzed. In addition the lung tissue was evaluated histopathologically. Boric acid application was found to significantly enhance the positive effect of antioxidant enzymes. Normal histological structure was better preserved, some of macrophages were non-activated, and a remarkable reduction of neutrophil infiltration was seen after treatment in boric acid given group.

Keywords

• Antioxidant enzyme,boric acid,histopathology,ischemia/reperfusion,lung damage

Sıçanlarda Alt Ekstremite İskemi Reperfüzyonu Sonucunda Gelişen Uzak Doku Akciğer Hasarının Önlenmesinde Borik Asidin Etkilerinin İncelenmesi

Abstract

Bu çalışmada, alt ekstremite iskemi reperfüzyon hasarı sebebiyle akciğerlerde meydana gelen biyokimyasal ve histopatolojik değişiklikler ve bunlara karşı borik asidin koruyucu etkisi araştırıldı. Sıçanlar Grup 1 (Kontrol/sham grubu, n=9), Grup 2 (İskemi-reperfüzyon grubu, I/R, n=9) ve Grup 3 (İskemi-reperfüzyon, (I/R) + Borik asit (BA), n=9) olmak üzere üç gruba ayrıldı. İskemi-reperfüzyon prosesi olmayan ilk sıçan grubuna sadece anestezi uygulandı. İkinci gruba iki saatlik bir iskemi sonrası, sol alt ekstremite turnike yardımıyla iki saatlik reperfüzyon uygulandı. Üçüncü gruba, iskemi başlamadan 10 dakika önce 200 mg / kg (i.p.) borik asit uygulaması yapıldı, iki saatlik iskemi ve iki saatlik reperfüzyon uygulaması yapıldı. Akciğer dokusunda bazı antioksidan enzimlerin (SOD, GSH-Px, CAT) aktiviteleri analiz edildi. Ayrıca akciğer dokusu histopatolojik olarak değerlendirildi. Borik asit uygulamasının antioksidan enzimlerin pozitif etkisini önemli ölçüde güçlendirdiği tespit edildi. Borik asit verilen grupta tedaviden sonra normal histolojik yapının daha iyi korunduğu, bazı makrofajların aktive edilmediği ve nötrofil infiltrasyonun da belirgin bir azalmanın olduğu gözlemlendi.

Keywords

• Akciğer hasarı,antioksidan enzim,borik asit,histopatoloji,iskemi/reperfüzyon

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