Immunohistochemical Localization of S100 Protein in the Pre-pubertal and Pubertal Angora Rabbits Testis and Epididiymis

Abstract

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Keywords

• Ankara tavşanı, epididimis, S100 proteini, testis.

Pre-pubertal ve Pubertal Dönemlerde Ankara Tavşanı Testis ve Epididimislerinde S100 Proteininin İmmunohistokimyasal Lokalizasyonu

Abstract

Çalışma, ülkemize has bir kültür hayvanı ve bilinen en eski tavşan ırkı olan Ankara tavşanının pre-pubertal ve pubertal dönem testis ve epididimislerinde S100 proteinin varlığını ve lokalizasyonunu immunohistokimyasal olarak belirlemek amacıyla planlanmıştır. Çalışmanın materyalini, altısı üç aylık (pre-pubertal dönem) ve altısı da sekiz aylık olan (pubertal dönem) toplam on iki adet sağlıklı, beyaz Ankara tavşanından alınan testis ve epididimisler oluşturdu. S100 proteinin lokalizasyonunun belirlenmesi amacıyla strept-ABC immunohistokimyasal boyama tekniği uygulandı. Pre-pubertal dönemdeki tavşan testislerinde, seminifer tubül duvarındaki hücrelerden sadece Sertoli hücreleri ile intertubüler alanlarda bazı Leydig hücrelerinde S100 pozititivesi belirlendi. Pubertal dönemdeki tavşan testislerinde ise, seminifer tubülusların duvarındaki Sertoli hücrelerinde, terminal seğmentin modifiye Sertoli hücrelerinde ve tubulus rektus epitelinde S100 pozitif immunreaksiyon gözlendi. Multifonksiyonel özellikteki S100 proteinin Ankara tavşanı testislerindeki hücresel lokalizasyonunun laboratuvar hayvanları, primatlar ve karnivorlardan daha çok domuz ve ruminant testislerine benzerlik gösterdiği sonucuna varıldı

Keywords

• Ankara tavşanı, epididimis, S100 proteini, testis.

References

1. Amselgruber W, Sinowatz F, Schams D, Lehmann immunoreactivity in bovine testis. Andrologia, 24: 231-235. S-100 protein
2. Amselgruber W, Sinowatz F, Erhard M, 1994. Differential distribution of immunoreactive S- 100 Histochemistry, 102: 241-245. mammalian testis.
3. Bock E, 1978. Nervous system spesific proteins. J Neurochem, 30(1): 7-14.
4. Cruzana BC, Hondo E, Kitamura N, Matsuzaki S, Nakagawa M, Yamada J, 2000. Differential localization of immunoreactive α- and β- subunits of S-100 protein in feline testis. Anat Histol Embryol, 29(2): 83-86.
5. Cruzana BC, Budipitojo T, De Ocampo G, Sasaki M, Kitamura N, Yamada J, 2003. Immunohistochemical distribution of S-100 protein and subunits (S100-α and S100-β) in the swamp-type water buffalo (Bubalus bubalis) testis. Andrologia, 35(3): 142-145.
6. Czykier E, Sawicki B, Zabel M, 1999. Immunohistochemical localization of S-100 protein in the European bison testis and epididiymis. Folia Histochem Cytobiol, 37(2): 83-84.
7. Czykier E, Sawicki B, Zabel M, 2000. S-100 protein immunorectivity in mammalian testis and epididiymis. Folia Histochem Cytobiol, 38 (4): 163-166.
8. Donato R, 1999. Functional roles of S100 proteins, calcium-binding proteins of the EF- hand type. BBA, 1450(3): 191-231.

9. Donato R, 2001. S100. a multigenic family of calcium-modulated proteins of the EF-hand type with intracellular and extracellular functional roles. Int J Biochem Cell Biol, 33(7): 637-668.
10. Donato R, 2003. Intracellular and extracellular roles of S100 proteins. Microsc Res Tech, 60 (6): 540-551.
11. Girod C, Durand N, Raccurt M, 1986. A comparative study of immunocytochemical localization of S-100 protein in the monkey (Macaca irus) and albino rat testis. Biomed Res, 7: 333-337.
12. Griswold MD, 1998. The central role of Sertoli cells in spermatogenesis. Sem Cell Dev Biol, 9(4): 411-416.
13. Haimato H, Hosoda S, Kato K, 1987. Differential distribution of immunoreactive S100-α and S100-β proteins in normal nonnervous human tissues. Lab Invest, 57(5): 489-498.
14. Heizmann CW, Cox J, 1998. A new perspectives on S100 proteins: a multi- functional Ca2+, Zn2+and Cu2+ binding protein family. BioMetals, 11: 383-397.
15. Heizmann CW, Fritz G, Schafer BW, 2002. S100 proteins: structure, functions and pathology. Front Biosci, 1(7): 1356-1368.
16. Hsu S, Raine L, Fanger H, 1981. Use of Avidin-Biotin Peroxidase Complex (ABC) in immunoperoxidase comparasion between ABC and unlabelled antibody (PAP)procedures. J Histochem Cytochem, 29(4): 577-580. a
17. Huerkamp MJ, 2003. The rabbit. In: B. Ballard R. Cheek (1st Ed), Exotic Animal Medicine for the Veterinary Technician. Iowa State Press, Ames, Iowa, pp 192-196.
18. Kagi U, Chafoulleas JG, Norman AW, Heizmann appearance of the Ca2+-binding proteins parvalbumin, calbindin, D-28K, S-100 proteins and calmodulin during tasticular development in the rat. Cell Tissue Res, 252(2): 359-365.
19. Marenholz I, Lovering RC. Heizmann CW, 2006. An update of the S100 nomenclature. BBA, 1763(11): 1282-1283.
20. Michetti F, Lauriola L, Rende M, Stolfi VM, Battaglia F, Cocchia D, 1985. S-100 protein in the immunohistochemical study. Cell Tissue Res, 240(1): 137-142. and
21. Mcnutt NS, 1998. S100 family of multipurpose calcium-binding proteins. J Cutan Pathol, 25 (10): 521-529.
22. Moore B, 1965. A soluable protein characteristic of the nervous system. Biochem Biophys Res Commun, 19(6): 739-744.
23. Ortega HH, Lorente JA, Salvetti NR 2004. Immunohistochemical study of intermediate filaments expression in Leydig cells of laboratory rodents. Anat Histol Embryol, 33(5): 309-315.
24. Osman DI, 1979. A comparative ultrastructural study on typical and modified Sertoli cells before and after ligation of the efferent ductules in the rabbit. Anat Histol Embryol, 8 (2): 114-123.
25. Santamaria-Kisiel L, Rintala-Dempsey AC, Shaw GS, 2006. Calcium dependent and – independent interactions of the S100 protein family. Biochem J, 396: 201-214.
26. Sugimura M, Miura M, Suzuki Y, Atoji Y, 1989. S-100 immunoreactive cells in non-nervous duck tissues. Avian Pathol, 18(3): 503-510.
27. Zimmer DB, Cornwall EH, Landar A, Song W, 1995. The S100 protein family: history, function and expression. Brain Res Bull, 37(4): 417-429.
28. Zimmer DB, Sadosky PW, Weber DJ, 2003. Molecular mechanism of S100-target protein interactions. Microsc Res Tech, 60(6): 552- 559.