Kadmiyum Uygulanan Farelerde Oluşturulan Oksidatif Strese Karşı Resveratrolün Koruyucu Etkisinin Araştırılması*

Abstract

Kadmiyum ve diğer ağır metaller hem çevresel hem de mesleki maruziyetlerle insan sağlığını tehdit etmekte ve sanayide yaygın olarak kullanılmaktadır. Resveratrol anti-inflamatuvar, antioksidan, antitümör ve immunomodulatör özellikleri olan doğal bir polifenolik bileşiktir. Bu nedenle yapılan çalışmada farelerin plazma, karaciğer, böbrek ve kalp dokusunda kadmiyum toksisitesine karşı resveratrolün koruyucu etkisi araştırılmıştır. Çalışmada 35 fare eşit olarak 5 gruba ayrılmıştır. Kontrol grubu, alkol grubu, kadmiyum grubu (CdCl2, 1 mg/kg/gün), resveratrol grubu (10 mg/kg/gün), kadmiyum+resveratrol grubu (1 mg/kg/gün+10 mg/kg/gün) olarak belirlenmiştir. Tüm uygulamalar 21 gün süreyle oral gavaj yoluyla uygulanmıştır. Uygulama süresi sonunda karaciğer, böbrek ve kalp dokusunda oksidatif stresin gösterge-si olarak malondialdehit (MDA) ve nitrik oksit (NO) miktarları, antioksidan savunmanın biyobelirteci olarak redükte glu-tatyon (GSH) miktarı, akut faz yanıtın bir göstergesi olan total siyalik asit (TSA) ve plazmada aspartat aminotransferaz (AST), alanin aminotransferaz (ALT), gamma glutamil transpeptidaz (GGT) aktiviteleri spektrofotometrik yöntemlerle belirlenmiştir. Kontrole göre, kadmiyum verilen grupta doku (karaciğer, böbrek ve kalp) MDA, NO, TSA düzeyleri ile plazma AST, ALT, GGT aktiviteleri önemli derecede artarken, doku GSH düzeyi ise önemli derecede azalmıştır. Kad-miyum+resveratrol verilen grupta ise doku MDA, NO, TSA düzeyleri ile plazma AST, ALT, GGT aktivitelerinin kadmi-yum verilen gruba göre istatistiksel olarak önemli derecede azalırken, doku GSH düzeyi ise önemli derecede artmıştır. Sonuç olarak kadmiyumun toksik etkisine karşı resveratrolün karaciğer, böbrek ve kalpte koruyucu etkisinin olabileceği kanısına varılmıştır.

Keywords

• Kadmiyum
• oksidatif stres
• resveratrol
• siyalik asit

The Investigation of the Protective Effect of Resveratrol on Oxidative Stress Levels in Mice Given Cadmium

Abstract

Cadmium and other heavy metals which are commonly used in industry could threaten human health with both environmental and occupational exposures. Resveratrol, a natural polyphenol, is thought to have some properties such as anti-inflammatory, antioxidant, antitumor and immunomodulatory. Therefore, the present study was designed to examine the protective effect of resveratrol on cadmium toxicity in plasma, liver, kidney and heart tissue of mice. In our study, thirty five mice were equally divided into five groups. Control group, alcohol group, cadmium group (CdCl2, 1 mg/kg/day), resveratrol group (10 mg/kg/day), cadmium+resveratrol group (1 mg/kg/day+10 mg/kg/day). All treatments were administered orally via gavage for 21 days. The amount of malondialdehyde (MDA) and nitric oxide (NO) as an indicator of oxidative stress in liver, kidney, and heart tissue at the end of the application period, the amount of reduced glutathione (GSH) as a biomarker of antioxidant defense, total sialic acid (TSA) as an indicator of acute phase respon-se and aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma glutamyl transpeptidase (GGT) activities in plasma were determined by spectrophotometric methods. In cadmium group, it was determined that tissue MDA, NO, TSA level and plasma AST, ALT, GGT activities increased significantly compared to control in the tissue (liver, kidney, and heart), while tissue GSH level decreased significantly compared to control. In cadmium+resveratrol group, it was determined that tissue MDA, NO, TSA level and plasma AST, ALT, GGT activities decreased, while tis-sue GSH level increased statistically significantly compared to cadmium groups. As a result, it was concluded that res-veratrol may have a protective effect on the liver, kidney and heart against the toxic effect of cadmium.

Keywords

• Cadmium
• oxidative stress
• resveratrol
• sialic acid

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