Bir Kıl Keçisinde Nöronal Lipofusinozis

Abstract

Bu olgu raporunda, üç yaşlı, dişi bir kıl keçisinde görülen nöronal lipofusinozisin tanımlanması amaçlandı. Keçinin sistemik nekropsisi yapıldıktan sonra alınan doku örneklerinin kesitleri; lipofusin pigmenti için hematoksilen-eozin (HE), periodic acid-schiff (PAS), Schmorl’s ve long Ziehl-Neelsen (ZN) boyama yöntemleri ile; hemosiderin için Turnbull blue ve melanin için melanin removal method II yöntemleri ile boyandıktan sonra ışık mikroskobunda değer-lendirildi. Herhangi bir histokimyasal uygulama görmemiş preparatlar direkt olarak floresan mikroskopta; ayrıca pons ve medulla oblongata’dan alınan doku örnekleri elektron mikroskopta incelendi. Makroskobik incelemede, meninksler matlaşmış ve serebral hemisferler hafif sert kıvamlı idi. Mikroskobik incelemede, pons ve medulla oblongata’da bulunan nöronların sitoplazmalarında değişen yoğunluklarda kırmızı ya da sarı-kahve renkli pigment granülleri görüldü. Bu granüller sıklıkla perinüklear yerleşimli olup, bazı nöronlarda sitoplazmanın tamamını doldurmuştu. Pigment birikimi ile birlikte meningeal ve perinöronal ödem, perivasküler kanama ve nöron dejenerasyonu belirlendi. PAS, Schmorl’s ve Long ZN boyama yöntemleri ile pozitif; Turnbull blue ve melanin removal method II boyama yöntemleri ile negatif bo-yanma özelliği gösteren pigment granüllerinin tamamı, floresan mikroskoptaki direkt incelemede floresan pozitif yan-sımalar verdi. Elektron mikroskobik incelemede de bu pigment granüllerinin lipofusin pigmenti olduğu doğrulandı.

Keywords

• Nöronal lipofusinozis
• patoloji
• elektron mikroskopi
• keçi

Neuronal Lipofuscinosis in a Hairy Goat

Abstract

In the present case, neuronal lipofuscinosis was determined in a three-year-old female hair goat. The sys-temic necropsy was performed. Tissue sections were stained with hematoxylin and eosin (HE), periodic acid schiff stain (PAS), Schmorl’s and long Ziehl-Neelsen (ZN) methods for lipofuscin; Turnbull’s blue method for hemosiderin and melanin removal method II for melanin. Unstained deparaffinized sections were examined microscopically for fluores-cence in transmitted ultraviolet light. In addition, samples of pons and medulla oblongata were collected and processed for transmission electron microscopy investigations. Macroscopically, the meninges were opaque and the cerebral hemispheres had firm consistency. Microscopically, pigment granules in varying intensity were observed in the cytop-lasm of the neurons of the pons and medulla oblongata. Red or yellowish-brown grannules were usually seen in peri-nuclear localization, and filled all cytoplasm of some neurons. Moreover, meningeal and perineuronal edema, perivas-cular hemorrhage and neuronal degeneration were prominent histopatologic findings. The granules were positively stained with PAS, Schmorl’s and long ZN methods, but negatively stained with Turnbull’s blue method and melanin removal method II. Pigments found in all affected neurons were brightly autofluorescent. Electron microscopy also confirmed that the pigment granules were lipofuscin.

Keywords

• Neuronal lipofuscinosis
• pathology
• electron microscopy
• goat

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