Investigation of Relationship Between Coagulation Parameters and Embryonic Loss in Embryo Transferred Cows and Heifers

Year 2021, , 84 - 88, 01.08.2021

Öznur Aslan Kutlay Gürbulak Uğur Kara Erkan Say , Esra Canooğlu Murat Abay

https://doi.org/10.32707/ercivet.952884

Abstract

The aim of the study, the relationship between coagulation parameters and embryonic loss in embryo transferred (ET) cows and heifers wereevaluated. The animal material of this study consisted of 19 cows and 19 heif-ers on farms located in East Mediterranean Agriculture Research Institute in Adana. Blood samples were collected before the ET application from the recipient cows and heifers. Coagulation parameters measured included prothrombin time (PT), activated thromboplastin time (APTT), fibrinogen, thrombin time (TT), anti-thrombin-III (AT3) and D-dimer using by Sysmex® CA-7000 and activated protein C resistance, protein C and protein S using by Sysmex CS-5100. The pregnancy rate was found 5/19 (26.3%) and 5/19 (26.3%) in cows and in heifers, respectively (P>0.05). The em-bryonic mortality ratio in cows was 60% (3/5) and in heifers it was 40% (2/5). The differences of D-dimer levels be-tween pregnant and non-pregnant animals were significant (P<0.05).The APTT levels between the groups with preg-nancy and embryonic loss were significantly different (P<0.05). As a result, it was determined that there is a relation-ship between plasma D-Dimer levels and embryonic loss in cows that were transferred embryo.To the best of authors’ knowledge, this is the first study reporting the relationship between coagulation parameters and embryonic loss in ET cows and heifers.

Keywords

Bovine, coagulation parameters, embryonic loss, embryo transfer

Embriyo Transferi Yapılan İnek ve Düvelerde Koagulasyon Parametreleri ile Embriyonik Kayıp Arasındaki İlişkinin Araştırılması

Abstract

Çalışmanın amacı, embriyo transferi (ET) yapılan Holstein ırkı inek ve düvelerde, pıhtılaşma parametreleri ile embriyonik ölüm arasındaki ilişkinin araştırılmasıdır. Çalışmaya, Doğu Akdeniz Tarım Araştırmaları Enstitüsü (Adana) çiftliklerinde bulunan 19 inek ve 19 düve dahil edildi. Alıcı inek ve düvelerden ET yapıldığı gün transfer öncesinde kan örnekleri alındı. Protrombin zamanı (PZ), aktive edilmiş parsiyel tromboplastin zamanı (APTZ), fibrinogen, trombin za-manı (TZ), antitrombin III (AT3) ve D-dimer gibi koagulasyon parametreleri Sysmex® CA- 7000 ve aktive edilmiş protein C rezistansı, protein C ve protein S ise Sysmex CS-5100 kullanılarak ölçüldü. Çalışmada gebelik oranı ineklerde 5/19 (%26.3) ve düvelerde 5/19 (%26.3) olarak belirlendi. Embriyonik ölüm oranı ineklerde %60 (3/5), ise düvelerde %40 (2/5) olarak belirlendi (P>0.05). Gebe olan ve olmayan hayvanlar arasında D-dimer seviyeleri arasındaki fark önemli bulundu (P<0.05). Gebelik devam eden ve embriyonik kayıp belirlenen gruplar arasında APTT seviyeleri arasındaki fark önemli bulundu (P<0.05). Sonuç olarak, embriyo transferi yapılan sığırlarda plazma D-dimer seviyeleri ile embriyo-nik kayıp arasında ilişki olduğu belirlendi. Yazarların bilgisine göre sunulan çalışma embriyo transferi yapılan inek ve düvelerde koagulasyon parametrelerinin değerlendirildiği ilk çalışma olması açısından önemlidir.

Keywords

Embriyonik ölüm, embriyo transferi, koagulasyon parametreleri, sığır

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