Kayseri İlinde Tüketime Sunulan Tavuk Etlerinde Staphylococcus aureus ve Enterotoksin Varlığının Araştırılması*
Year 2015,
Volume: 12 Issue: 2, 93 - 98, 01.08.2015
Hüseyin Kaya
Nurhan Ertaş Onmaz
,
Zafer Gönülalan
,
Serhat Al
Abstract
Bu çalışmada Kayseri ilinde satışa sunulan tavuk etlerinde Staphylococcus aureus (S. aureus) ve enterotoksinlerinin varlığı
incelendi. Çalışmada toplam 100 adet tavuk karkas örneği materyal olarak kullanıldı. Çalışmada incelenen 100 tavuk karkasının
30’unun (%30) S. aureus ile kontamine olduğu ve kontaminasyon düzeyinin 2x101
kob/g ile 1x108
kob/g arasında olduğu belirlendi. S.
aureus ile kontamine tavuk eti örneğinden üçünün (%10) Türk Gıda Kodeksi Et ve Et Ürünlerindeki Mikrobiyolojik Limitler Tebliği’nin
üzerinde olduğu tespit edildi. Çalışmada, incelenen tavuk karkası örneklerinden elde edilen S. aureus kolonilerine uygulanan ELISA
testi sonucunda izolatların hiç birinde Stafilokokal enterotoksinlere rastlanmadı. Bu çalışmada elde edilen verilere göre, Kayseri’de
satışa sunulan tavuk karkas etinde S. aureus kontaminasyonu olabileceği ve bu durumun halk sağlığı açısından göz ardı edilmemesi
gereken bir tehlike olduğu sonucuna varıldı.
References
- 1. Alvarez-Astorga M, Capita R, Alonso-Calleja C, Moreno B, Garcia Fernandez MC. Microbiological quality of retail chicken by-products in Spain. Meat Sci 2002; 62(1): 45-50.
- 2. Bilge F, Karaboz İ. İzmir’de piyasada açıkta satışa sunulan bazı gıdaların Staphylococcus aureus ve enterotoksinleri bakımından incelenmesi. Orlab On-Line Mikrobiyoloji Dergisi 2000; 3: 6-11.
- 3. Bystron J, Molenda J, Bania J, Kosek-Paszkowska K, Czerw M. Occurrence of enterotoxigenic strains of Staphylococcus aureus in raw poultry meat. Pol J Vet Sci 2005; 8(1): 37-40.
- 4. Efe M, Gümüşsoy KS. Ankara garnizonunda tüketime sunulan tavuk etlerinin mikrobiyolojik analizi. ERÜ Sağlık Bil Derg 2005; 14(13): 151-7.
- 5. Erol İ. Gıda Hijyeni ve Mikrobiyolojisi, Pozitif Matbaacılık, Ankara, 2007: sf. 139.
- 6. Gonzales-Miret ML, Escudero-Gilete ML, Heredia FJ. The establishment of critical control points at the washing and air chilling stages in poultry meat production using multivariate statistics. Food Control 2006; 17(12): 935-41.
- 7. Hang’ombe BM, Sharma NR, Skjerve E, Tuchili LM. Isolation of bacteria during processing of chicken carcasses for the market in Lusaka, Zambia. Veterinarski Arhiv 1999; 69: 191-7.
- 8. Kitai S, Shimizu A, Kawano J, Sato E, Nakano C, Kitagawa H, Fujio K, Matsumura K, Yasuda R, Inamoto T. Prevalence and characterization of Staphylococcus aureus and enterotoxigenic Staphylococcus aureus in retail raw chicken meat throughout Japan. J Vet Med Sci 2005; 67(3): 269-74.
- 9. Koçyiğit A. İzmir'de çeşitli marketlerde satışa sunulan tavuk ve hindi etlerinde Staphylococcus aureus aranması, sayımı ve tanımlanması. Yüksek Lisans Tezi, E.Ü. Fen Bilimleri Enstitüsü, İzmir 2002: 63.
- 10. Koluman A, Ünlü T, Dikici A, Tezel A, Akçelik EN, Burkan ZT. Presence of Staphylococcus aureus and Staphylococcal Enterotoxins in Different Foods. Kafkas Univ Vet Fak Derg 2011; 17(Suppl A): 55-60.
- 11. Kozačinski L, Hadžiosmanović M, Zdolec N. Microbiological quality of poultry meat on the Croatian market. Veterinarski Arhiv 2006; 76: 305-13.
- 12. Lee JA, Hataway SC. The challenge designing valid HACCP plans for raw food commodities. Food Control 1998; 9(2): 111-7.
- 13. Marthenge JM, Ombui JN. Detection of staphylococcal enterotoxins in milk and meat in Nairobi Kenya using Enzymes Linked Immunosorbent Assay. J Trop Microbiol Biotech 2007; 3(1): 23-8.
- 14. Mead GC. Microbiological quality of poultry meat: A review, Braz J Poult Sci 2004; 6(3): 135-42.
- 15. Normanno G, Firinu A, Virgilio S, Mula G, Dambrosio A, Poggiu A, Decastelli L, Mioni R, Scuota S, Bolzoni G, Di Giannatale E, Salinetti AP, La Salandra G, Bartoli M, Zuccon F, Pirino T, Sias S, Parisi A, Quaglia NC, Celano GV, Coagulase-positive Stphylococci and Staphylococcus aureus in food products marketed in Italy. Int J Food Microbiol, 2005; 98(1): 73-9.
- 16. Panisello PJ, Rooney R, Quantick P, Stanwell-Smith R. Application of foodborne disease outbreak data in the development and maintenance of HACCP systems. Int J Food Microbiol 2000; 59(3): 221-34.
- 17. Park CE, Akhtar M, Rayman MK. Evaluation of a commercial enzyme immunoassay kit (RIDASCREEN) for detection of staphylococcal enterotoxins A, B, C, D, and E in foods. Appl Environ Microbiol 1994; 60(2): 677-81.
- 18. Pipová M, Turek P, Laciaková M, Ivanova M, Plachá I. Changes in microbial parameters during the production of fine poultry salami. Vet Med-Chezch 1997; 42: 81-5.
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Detection of Staphylococcus aureus and Their Enterotoxins in Poultry Meats Consumed in Kayseri
Year 2015,
Volume: 12 Issue: 2, 93 - 98, 01.08.2015
Hüseyin Kaya
Nurhan Ertaş Onmaz
,
Zafer Gönülalan
,
Serhat Al
Abstract
The aim of this study was to determine the presence of Staphylococcus aureus (S. aureus) and its enterotoxins in the
poultry consumed in Kayseri. 100 chicken carcasses were used as the material. Thirty out of 100 chicken carcasses sampled (30%),
found to be contaminated with S. aureus at the contamination level ranging from 2x101
kob/g to 1x108
kob/g. Three of the contaminated
poultry meat samples (10%), found to be contaminated with this pathogen at the levels exceeding the microbiological limits laid
down in Turkish Food Codex regarding meat and meat products. In this study, none of the staphylococcal enterotoxins were found in
S. aureus isolates that were obtained from chicken carcasses through ELISA. Consequently, the presence of S. aureus was effective
in carcasses retailed in Kayseri and vicinity and it was concluded that this situation should not be ignored for public health.
References
- 1. Alvarez-Astorga M, Capita R, Alonso-Calleja C, Moreno B, Garcia Fernandez MC. Microbiological quality of retail chicken by-products in Spain. Meat Sci 2002; 62(1): 45-50.
- 2. Bilge F, Karaboz İ. İzmir’de piyasada açıkta satışa sunulan bazı gıdaların Staphylococcus aureus ve enterotoksinleri bakımından incelenmesi. Orlab On-Line Mikrobiyoloji Dergisi 2000; 3: 6-11.
- 3. Bystron J, Molenda J, Bania J, Kosek-Paszkowska K, Czerw M. Occurrence of enterotoxigenic strains of Staphylococcus aureus in raw poultry meat. Pol J Vet Sci 2005; 8(1): 37-40.
- 4. Efe M, Gümüşsoy KS. Ankara garnizonunda tüketime sunulan tavuk etlerinin mikrobiyolojik analizi. ERÜ Sağlık Bil Derg 2005; 14(13): 151-7.
- 5. Erol İ. Gıda Hijyeni ve Mikrobiyolojisi, Pozitif Matbaacılık, Ankara, 2007: sf. 139.
- 6. Gonzales-Miret ML, Escudero-Gilete ML, Heredia FJ. The establishment of critical control points at the washing and air chilling stages in poultry meat production using multivariate statistics. Food Control 2006; 17(12): 935-41.
- 7. Hang’ombe BM, Sharma NR, Skjerve E, Tuchili LM. Isolation of bacteria during processing of chicken carcasses for the market in Lusaka, Zambia. Veterinarski Arhiv 1999; 69: 191-7.
- 8. Kitai S, Shimizu A, Kawano J, Sato E, Nakano C, Kitagawa H, Fujio K, Matsumura K, Yasuda R, Inamoto T. Prevalence and characterization of Staphylococcus aureus and enterotoxigenic Staphylococcus aureus in retail raw chicken meat throughout Japan. J Vet Med Sci 2005; 67(3): 269-74.
- 9. Koçyiğit A. İzmir'de çeşitli marketlerde satışa sunulan tavuk ve hindi etlerinde Staphylococcus aureus aranması, sayımı ve tanımlanması. Yüksek Lisans Tezi, E.Ü. Fen Bilimleri Enstitüsü, İzmir 2002: 63.
- 10. Koluman A, Ünlü T, Dikici A, Tezel A, Akçelik EN, Burkan ZT. Presence of Staphylococcus aureus and Staphylococcal Enterotoxins in Different Foods. Kafkas Univ Vet Fak Derg 2011; 17(Suppl A): 55-60.
- 11. Kozačinski L, Hadžiosmanović M, Zdolec N. Microbiological quality of poultry meat on the Croatian market. Veterinarski Arhiv 2006; 76: 305-13.
- 12. Lee JA, Hataway SC. The challenge designing valid HACCP plans for raw food commodities. Food Control 1998; 9(2): 111-7.
- 13. Marthenge JM, Ombui JN. Detection of staphylococcal enterotoxins in milk and meat in Nairobi Kenya using Enzymes Linked Immunosorbent Assay. J Trop Microbiol Biotech 2007; 3(1): 23-8.
- 14. Mead GC. Microbiological quality of poultry meat: A review, Braz J Poult Sci 2004; 6(3): 135-42.
- 15. Normanno G, Firinu A, Virgilio S, Mula G, Dambrosio A, Poggiu A, Decastelli L, Mioni R, Scuota S, Bolzoni G, Di Giannatale E, Salinetti AP, La Salandra G, Bartoli M, Zuccon F, Pirino T, Sias S, Parisi A, Quaglia NC, Celano GV, Coagulase-positive Stphylococci and Staphylococcus aureus in food products marketed in Italy. Int J Food Microbiol, 2005; 98(1): 73-9.
- 16. Panisello PJ, Rooney R, Quantick P, Stanwell-Smith R. Application of foodborne disease outbreak data in the development and maintenance of HACCP systems. Int J Food Microbiol 2000; 59(3): 221-34.
- 17. Park CE, Akhtar M, Rayman MK. Evaluation of a commercial enzyme immunoassay kit (RIDASCREEN) for detection of staphylococcal enterotoxins A, B, C, D, and E in foods. Appl Environ Microbiol 1994; 60(2): 677-81.
- 18. Pipová M, Turek P, Laciaková M, Ivanova M, Plachá I. Changes in microbial parameters during the production of fine poultry salami. Vet Med-Chezch 1997; 42: 81-5.
- 19. Ünlütürk A, Turantaş F. Gıda mikrobiyolojisi. 3. baskı, Meta Basım, İzmir, 2003: 47- 51.