Yumurtacı Bıldırcınlarda Oluşturulan Isı Stresinde Krom ve Çinkonun Bazı Kan Parametrelerine Etkileri*

Year 2016, Volume: 13 Issue: 1, 38 - 49, 01.08.2016

Meryem Gültekin Şentürk Fatma Uyanık

Abstract

Bu çalışmada, ısı stresi oluşturulan yumurtacı bıldırcınların rasyonuna krom (Cr+3) ve çinkonun (Zn+2) ayrı ayrı ve
birlikte ilavesinin canlı ağırlık, yem tüketimi, yumurta verimi ile kan glikoz, lipid ve malondialdehit (MDA) düzeylerine etkisi
belirlenmiştir. Sekiz haftalık 240 adet Japon bıldırcını, her birinde 48’er hayvan bulunan beş deneme grubuna ayrılmıştır.
Birinci (termonötral) ve ikinci (ısı stresi) gruplar kontrol olarak tutulmuş ve bazal rasyonla; deneme grupları 20 mg/kg Cr, 40
mg/kg Zn, 20 mg/kg Cr + 40 mg/kg Zn ilaveli bazal rasyonlarla sekiz hafta beslenmiştir. Denemenin 5. ve 8. haftalarında,
hayvanlardan alınan kan örneklerinde serum glikoz, trigliserit, total kolesterol, yüksek dansiteli lipoprotein (HDL) ve plazma
MDA analizleri yapılmıştır. Isı stresinin canlı ağırlık ve yem tüketimini önemli, yumurta verimini ise önemsiz düzeyde azalttığı;
yeme Cr, Zn veya Cr + Zn ilavesinin canlı ağırlık, yem tüketimi ve yumurta verimini olumlu yönde etkilemediği gözlenmiştir.
Isı stresinin serum glikoz düzeyini hafifçe arttırdığı ve bu artışın da yeme Cr ilavesiyle azaldığı saptanmıştır. Isı stresi serum
trigliserit düzeyini azaltırken total kolesterol düzeyini artırmış; yeme Cr ve Cr + Zn ilavesiyle trigliserit ve total kolesterolün
azaldığı gözlenmiştir. Yüksek dansiteli lipoprotein düzeyi ısı stresinden etkilenmezken Cr ilavesiyle artmıştır. Isı stresinin
etkisiyle plazma MDA düzeyinde gözlenen artma yeme Cr, Zn ya da her ikisinin birlikte ilavesiyle azalmıştır. Sonuç olarak, ısı
stresinin yumurtacı bıldırcınlarda canlı ağırlık, yem tüketimi ve yumurta verimini olumsuz etkilediği, serum parametrelerinde
değişikliklere yol açtığı ve plazma MDA düzeyini artırdığı; yeme Cr ve Zn ilavesinin, ısı stresi oluşturulan bıldırcınlarda azalan
yem tüketimi, canlı ağırlık ve yumurta verimini olumlu yönde etkilemediği, Cr’un serum glikoz, trigliserit ve total kolesterol
düzeylerindeki değişimleri düzeltebileceği ve özellikle plazma MDA düzeyini olumlu yönde etkileyebileceği söylenebilir.

Keywords

Çinko, ısı stresi, kan parametreleri, krom

Effects of Chromium and Zinc on Blood Parameters in Laying Quails Exposed to Heat Stress

Abstract

This study was performed to investigate the effects of separate and combined dietary supplementation of chromium
(Cr+3) and zinc (Zn+2) on live weight, food consumption, egg production, blood glucose, lipids and malondialdehyde (MDA)
levels of laying quails exposed to heat stress. Eight week-old 240 Japanese quails were assigned into five groups containing
48 birds in each. First (thermoneutral) and second (heat stress) groups were kept as controls and were fed on basal diets
and the treatment groups were fed on basal diets supplemented with 20 mg/kg Cr, 40 mg/kg Zn, 20 mg/kg Cr + 40 mg/kg Zn
for eight weeks. At 5th and 8th weeks, blood samples collected from the quails were analysed for serum glucose, triglycerides,
total cholesterol, high density lipoproteins (HDL) and plasma MDA. Heat stress resulted in significant reductions in live weight
and food consumption and a slight reduction in egg production, and supplementation of Cr, Zn and Cr + Zn had no significant
effects on live weight, food consumption and egg production. The slightly increased glucose level due to heat stress was decreased
by Cr supplementation. Heat stress decreased serum triglycerides and increased cholesterol, and these parameters
were reduced by Cr and Cr + Zn supplementation. Heat stress had no effect on HDL level but Cr increased the HDL. Supplementation
of Cr, Zn and their combination decreased the elevated MDA levels. In conclusion, heat stress adversely affected
live weight, food consumption and egg production, changed serum parameters and increased plasma MDA; supplementation
of Cr and Zn to the diets had no positive effects on decreased live weight, food consumption and egg production while Cr may
improve the changes in serum glucose, triglycerides and total cholesterol as well as plasma MDA in quails exposed to heat
stress.

Keywords

Blood parameters, chromium, heat stress, zinc

References

• 1. Abou El-Soud SB, Ebeid TA, Eid YZ. Physiological and antioxidative effects of dietary acetyl salicylic acid in laying japanese quail (Coturnix japonica) under high ambient temperature. J Poult Sci 2006; 43(3): 255-65.
• 2. Abu-Dieyeh ZHM. Effect of chronic heat stress and long-term feed restriction on broiler performance. Int J Poult Sci 2006; 5(2): 185-90.
• 3. Abu-Dieyeh ZHM. Effect of high temperature per se on growth performance of boilers. Int J Poult Sci 2006; 5(1): 19-21.
• 4. Aksoy TF. Yaz sıcağında kümes yönetimi. Vilsan Dergi 2006; 2: 4-6.
• 5. Anderson RA, Roussel AM, Zouari N, Mahjoub S, Matheau JM, Kerkeni A. Potential antioxidant effects of zinc and chromium supplementation in people with type 2 diabetes mellitus. Am Coll Nutr 2001; 20(3): 212-8.
• 6. Anonim. İlaçlı Yem İlaveleri ve Stres. Türkiye Ticaret Odaları, Sanayi Odaları ve Ticaret Borsaları Birliği Matbaası, Ankara 1970.
• 7. Anonim. How to understand heat stress and what to do about it. Rural Chemical Industries (AUST) Pty Ltd: http://www.heatstress.info. Erişim tarihi: 20.02.2007
• 8. Arad Z, Marder J, Eylath U. Serum electrolyte and enzyme responses to heat stress and dehydration in the fowl (Gallus domesticus). Comp Biochem Physiol A. 1983; 74(2): 449-453.
• 9. Arjona AA, Denbow DM, Weaver WD. Effects of heat stres early in life on mortality of broilers exposed to high environmental temparatures just prior to marketing. Poultry Science 1988; 67(2): 226-31.
• 10. Arslan A, Duru M. Kanatlılarda sıcaklık stresinin yönetilmesinde besleme açısından alınacak önlemler. MKU Ziraat Fak Derg 2004; 9(1-3): 93-100.
• 11. Berger LL. Trace minerals: Keys to immunity. Salt Institute 1996; 28(1): 1-4.
• 12. Bollengier-Lee S, Mitchell MA, Utomo DB, Williams PEV, Whitehead CC. Influence of high dietary vitamin E supplementation on egg production and plasma characteristics in hens subjected to heat stress. Br Poult Sci 1998; 39(1): 106-12.
• 13. Coppinger TR, Minton JE, Reddy PG, Blecha F. Repeated restraint and isolation stress in lambs increases pituitary-adrenal secretions and reduces cell-mediated immunity. J Anim Sci 1991; 69(7): 2808-14.
• 14. Çevik A. Broilerlerde Bakır Noksanlığına Bağlı Oksidatif Strese Karşı Antioksidan Yanıt. Doktora Tezi. İstanbul Üniv. Sağlık Bilimleri Enstitüsü, Biyokimya Programı. İstanbul-Türkiye, 2004.
• 15. Dabbert CB, Lochmiller RL, Teeter RG. Thermal stress influences clinical chemistry values of northern bobwhite (Colinus virginiaus). Comp Haematol Int 1996; 6(2): 120-2.
• 16. Donoghue DJ, Krueger BF, Hargis BM, Miller AM, el Halawani M. Thermal stress reduces serum luteinizing hormone and bioassayable hypothalamic content of luteinizing hormone-releasing hormone in hens. Biol Reprod 1989; 41(3): 419-24.
• 17. Durgun Z, Keskin E. The changes associated with fasting and acute heat stress on body temperature, blood acid-base balance and some parameters of japanese quail. Indian Vet 1998; 75(4): 299-303.
• 18. Emery DA, Vohra P, Ernst RA, Morrison, SR. The effect of cyclic and constant ambient temperatures on feed consumption, egg production, egg weight, and shell thickness of hens. Poultry Sci 1984; 63(10): 2027-35.
• 19. Fang YZ, Yang S, Wu G. Free radicals, antioxidans and nutrition. Nutr 2002; 18(10): 872-9.
• 20. Fujii J, Iuchi Y, Okada F. Fundamental roles of reactive oxygen species and protective mechanisms in the female reproductive system. Reproduc Biol Endocrinol 2005; 3: 43.
• 21. Garriga C, Hunter RR, Amat C, Planas JM, Mitchell MA, Moreto M. Heat stress increases apical glucose transport in the chicken jejunum. Am J Physiol Regul Integr Comp Physiol 2006; 290: 195-201.
• 22. Herbert S, Prinzinger R. The ontogeny of endothermic reactions in the precocial domestic fowl (Gallus gallus domesticus) - already an embriyonic phenomenon? Symposium on Avian Thermal Physiology and Energetics, August, 8-14, 1998; Oulu- Finland. (Abstract).
• 23. John TM, George JC. Blood levels of cyclic AMP, thyroxine, uric acid, certain metabolites and electrolytes under heat-stress and dehydration in the pigeon. Arch Int Physiol Biochim 1977; 85(3): 571-82.
• 24. Keçeci T, Kocabatmaz M. Horozlarda stress ve askorbik asidin bazı kan metabolitleri üzerindeki etkisi. Vet Bil Derg 1995; 11(2): 29-33.
• 25. Kolsuz SŞ, Uyanık F. Yumurta tavuklarında yeme krom ilavesinin serum kalsiyum, fosfor, magnezyum ve çinko düzeylerine etkisi. Erciyes Üniv Sağlık Bilim Derg 2002; 11(2): 67-75.
• 26. Kolsuz AH, Uyanık F. Yumurta tavuklarında yeme krom ilavesinin serum lipid düzeylerine etkisi. Erciyes Üniv Sağlık Bilim Derg 2002; 11(2): 25-34.
• 27. Krausz S, Marder J, Eylath U. Physiological responses of dogs on exposure to hot, arid conditions. Serum constituents. Pflugers Arch 1977; 370(3): 287-9.
• 28. Krόliczewska B, Zawadzki W, Dobrzanski Z, Kaczmarek-Oliwa A. Changes in selected serum parameters of broiler chicken fed supplemental chromium. J Anim Physiol Anim Nutr 2004; 88(11-12): 393-400.
• 29. Kuczyński T. The application of poultry behaviour responses on heat stress to improve heating and ventilation systems efficiency. Electronic J Polish Agri Univ, Agri Eng 2002; 5(1): http://www.ejpau.media.pl/series/volume5/issue1/engineering/art-01.html. Erişim tarihi: 18.11.2006
• 30. Kutlu HR. Sıcaklık stresine maruz kalan etlik piliçlerin performanslarının korunmasında beslemenin önemi. Farmavet Bülten 1996; 12(3): 1-8.
• 31. Lien T, Chen S, Shiau S, Froman D, Hu CY. Chromium picolinate reduces laying hen serum and egg yolk cholesterol. Profess Anim Sci 1996; 12(2): 77-80.
• 32. Lien TF, Horng YM, Yang KH. Performance, serum charactesictics, carcase traits and lipid metabolism of broilers as affected by supplement of chromium picolinate. Br Poult Sci 1999; 40(3): 357-63.
• 33. Lien TF, Wu CP, Wang BJ, Shiao MS, Shiao TY, Lin BH, Lu JJ, Hu CY. Effect of supplemental levels of chromium picolinate on the growth performance, serum traits, carcass characteristics and lipid metabolism of growing-finishing pigs. Anim Sci 2001; 72: 289-96.
• 34. Marsden A, Morris TR, Cromarty AS. Effects of constant environmental temperatures on the performance of laying pullets. Br Poult Sci 1987; 28(3): 361-80.
• 35. Mengi A. Organizma direncinin stres ve beslenmeyle değişimi. İstanbul Üniv Vet Fak Derg 1989; 15(1): 81-9.
• 36. Moonsie-Shageer S, Mowat DN. Effect of level of supplemental chromium on performance, serum constituents, and immune status of stressed feeder calves. J Anim Sci 1993; 71(1): 232-8.
• 37. Moreno IM, Mate A, Repetto G, Vázquez CM, Cameán AM. Influence of microcystin –LR on the activity of membrane enzymes in rat intestinal mucosa. J Physiol Biochem. 2003; 59(4): 293-300.
• 38. Nazifi S, Saeb M, Rowghani E, Kaveh K. The influences of thermal stress on serum biochemical parameters of Iranian fat-tailed sheep and their correlation with triiodothyronine (T3), thyroxine (T4) and cortisol concentrations. Comp Clin Path 2003; 12: 135-9.
• 39. Offenbacher EG, Pi-Sunyer FX. Chromium in human nutrition. Ann Rev Nutr 1988; 8: 543-563.
• 40. Prasad SA, Küçük O. Zinc in cancer prevention. Cancer Metast Rev 2002; 21: 291-5.
• 41. Ramnath V, Rekha PS, Sujatha KS. Amelioration of heat stress induced disturbences of antioxidant defense system in chicken by Brahma Rasayana. eCAM 2008; 5(1): 1-8.
• 42. Roland DA, Bryant MM, Rabon HW. Influence of calcium and environmental temperature on performance of first-cycle (phase 1) commercial leghorns. Poultry Sci 1996; 75(1): 62-8.
• 43. Sands JS, Smith MO. Effect of dietary manganese proteinate or chromium picolinate supplementation on plasma insulin, glucagon, glucose and serum lipids in broiler chickens reared under thermoneutral or heat stres condutions. Int J Poult Sci 2002; 1(5): 145-9.
• 44. Sconberg S, Nockels CF, Bennett BW, Bruyninck W, Blancquaret AMB, Craig AM. Effects of shipping, handling, adrenocorticotropic hormone, and epinephrine on α-tocopherol content of bovine blood. Am J Vet Res 1993; 54(8): 1287-93.
• 45. Smith MO, Teeter RG. Potassium balance of the 5 to 8-week-old broiler exposed to constant heat or cycling high temperature stress and the effects of supplemental potassium chloride on body weight gain and feed efficiency. Poultry Sci 1987; 66(3): 487-92.
• 46. Smith KL, Waldron MR, Drackley JK, Socha MT, Overton TR. Performance of dairy cows as affected by prepartum dietary carbohydrate source and supplementation with chromium throughout the transition period. J Dairy Sci 2005; 88(1): 255-63.
• 47. Şahin K, Smith MO, Önderci M, Şahin N, Gürsu MF, Küçük O. Supplementation of zinc from organic orinorganic source improves performance and antioxdiadant status of heat distressed quail. Poultry Sci 2005; 84(6): 882-7.
• 48. Taştan R. Stresin immun sistem üzerine etkileri ve hayvan sağlığı yönünden önemi. Veteriner Mikrobiyoloji Derneği, Seri Konferanslar Ankara 1991: 2.
• 49. Teeter RG, Belay T. Broiler management during acute stres. Anim Feed Sci Technol 1996; 58(1-2): 127-42.
• 50. Utomo DB, Mitchell MA, Whitehead CC. Effects of α-tocopherol supplementation on plasma egg yolk precursor concentrations in laying hens exposed to heat stress. Br Poult Sci 1994; 35: 828.
• 51. Uyanık F. The effects of dietary chromium supplementation on some blood parameters in sheep. Biol Trace Elem Res 2001; 84(1-3): 93-101.
• 52. Vinson JA, Mandarano MA, Shuta DL, Bagchi M, Bagchi D. Beneficial effects of a novel IH636 grape seed proanthocyanidin extract and a niacin-bound chromium in a hamster atherosclerosis model. Mol Cell Biochem 2002; 240(1-2): 99-103.
• 53. Williamson RA, Misson BH, Davison TF. The effect of exposure to 40 degrees on the heat production and the serum concentrations of triiodothyronine, thyroxine, and corticosterone in immature domestic fowl. Gen Comp Endocrinol 1985; 60(2): 178-86.
• 54. Yahav S, Straschnow A, Plavnik I, Hurwitz, S. Blood system response of chickens to changes in environmental temperature. Poultry Sci 1997; 76(4): 627-33.
• 55. Yahav S, Luger D, Cahaner A, Dotan M, Rusal M Hurwitz, S. Thermoregulationin naked neck chickens subjected to different ambient temperatures. Br Poult Sci 1998; 39(1): 133-8.
• 56. Yıldız AÖ, Parlat SS, Yazgan O. The effects of organic chromium supplementation on production traits and some serum parameters of laying quails. Revue Med Vet 2004; 155(12): 642-6.