İnek Sütlerinden İzole Edilen Enterococcus faecalis ve Enterococcus faecium’un Vankomisin Direnci

Year 2016, Volume: 13 Issue: 2, 139 - 150, 04.08.2016

Tekin Kececı Kadir Semih Gumussoy Harun Hızlısoy

Abstract

 Bu çalışmada çiğ inek sütlerinden Enterococcus spp’lerin izolasyonu ve identifikasyonu ile elde edilen izolatlarda
vankomisin direncinin fenotipik ve moleküler yöntemlerle saptanması amaçlandı. Toplam 150 adet süt örneği sağlıklı
ve mastitisli hayvanlardan California Mastitis Test ile skorlanarak toplandı. Seksen dört Enterecoccus spp. izolatı
elde edildi ve bunların 57 (%68), 8 (%9) ve 19 (%23)’ü sırasıyla Enterococcus faecalis, Enterococcus faecium and
Enterococcus spp. olarak polimeraz zincir reaksiyonu ile identifiye edildi. Etest ile izolatların hiçbiri vankomisine
direnç göstermedi. Bununla birlikte, E. faecalis izolatlarının 11 (%19)’inde ve E. faecium izolatlarının 7(%88)’sinde
VanB geni pozitif olarak tespit edildi. Ayrıca, 1 izolatta VanC2, VanC3 genleri ve 2 E. faecium izolatında VanB, VanC2,
VanC3 genleri birlikte bulundu. Bu çalışmada inek sütlerinde önemli derecede Enterococcus spp. varlığı saptanmıştır.
Vankomisin direncinin moleküler testlerle tespitinden dolayı bu test, antibiyotik direncin tespitinde daha etkili olduğu
sonucuna varıldı

Keywords

Enterococcus spp., mastitis, PZR, sığır, süt, vankomisin

Vancomycin Resistance of Enterococcus faecalis and Enterococcus faecium Isolated from cattle milk

Abstract

In this study the presence of Enterococcus spp. in raw cattle milk and the detection of the resistance to
vancomycin of the isolates by using phenotypic and molecular methods were investigated. Totally, 150 milk samples
were collected from healthy animals or animals with mastitis scored with California Mastitis Test. Eighty four
Enterococcus spp. were isolated and 57 (68%), 8 (9%) and 19 (23%) of the isolates were identified by Polymerase
Chain Reaction as Enterococcus faecalis, Enterococcus faecium and Enterococcus spp., respectively. None of the
isolates were resistant to vancomycin with E test. However, 11 (19%) E. faecalis and 7 (88%) E. faecium isolates
were positive for VanB. VanC2, VanC3 and VanB, VanC2, VanC3 genes were found together in 1 and 2 E. faecium
isolates, respectively. In this study, Enterococcus spp. were significantly found in cattle milk. Because of the detection
of vancomycin resistance by molecular test, this method was found to be more effective in the detection of antibiotic
resistance.

Keywords

Cattle, Enterococcus spp., mastitis, milk, PCR, vancomycin

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