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Dexamethasonun 18 Günlük Embriyonik Civciv Bağırsağındaki Claudin Sıkı Bağlantı Proteinlerine Etkileri

Year 2018, Volume: 15 Issue: 1, 6 - 10, 15.04.2018

Abstract

Claudin (cldn) protein ailesi sıkı bağlantı yapılarının (tight junctions) temel birleşenidir ve sıkı bağlantı zincirlerinin
bariyer fonksiyonuna direk etki eder. Sıkı bağlantıların gelişmiş dokulardaki bariyer fonksiyonuna katkısının yanında, bu
bağlantı proteinleri, epitelin embriyonik gelişimi süresince mevcuttur ve normal gelişim için gereklidir. Glukokortikoitler
bağırsak dahil birçok dokunun embriyonik gelişimi için esastır. Aynı şekilde, sıkı bağlantıların bütünleşmesine uyarıcı
etkileri mevcuttur. Bu çalışmada, sentetik bir glukokortikoit olan dexamethasone (DEX)’in in vitro koşullarda 18 günlük
embriyonik civciv bağırsağındaki sıkı bağlantı proteinlerinin mRNA ekspresyonlarına bir etkisinin olup olmadığı kantitatif
Real-Time PCR (RT-PCR) kullanılarak araştırıldı. Henüz yumurtadan çıkmamış civciv bağırsağı bu çalışma için
uygun bir model olmaktadır, çünkü epitelyumdaki bariyer henüz tam olarak oluşmamış ve ayrıca bu bağırsak 24 saatlik
organ kültüründe morfolojik bütünlüğünü koruyabilmektedir. RT-PCR analizleri hem cldn-3 hem de cldn-5 sıkı bağlantı
proteinlerinin gen ekspresyonlarının DEX’in etkisiyle doza bağlı olarak arttığını ortaya çıkartmıştır.

References

  • 1. Black BL, Smith JE. Regulation of goblet cell-differentiation by calcium in embryonic chick intestine. Faseb J 1989; 3(14): 2653-9.
  • 2. Uni Z, Tako E, Gal-Garber O, Sklan D. Morphological, molecular, and functional changes in the chicken small intestine of the late-term embryo. Poultry Sci 2003; 82(11): 1747-54.
  • 3. Sonoda N, Furuse M, Sasaki H, Yonemura S, Katahira J, Horiguchi Y, Tsukita S. Clostridium perfringens enterotoxin fragment removes specific claudins from tight junction strands: evidence for direct involvement of claudins in tight junction barrier. J Cell Biol 1999; 147(1): 195-204.
  • 4. Van Itallie CM, Anderson JM. Claudins and epithelial paracellular transport. Annu Rev Physiol 2006; 68: 403-29.
  • 5. Colegio OR, Van Itallie CM, McCrea HJ, Rahner C, Anderson JM. Claudins create charge-selective channels in the paracellular pathway between epithelial cells. Am J Physiol-Cell Ph 2002; 283(1): C142-C7.
  • 6. Rahner C, Mitic LL, Anderson JM. Heterogeneity in expression and subcellular localization of claudins 2, 3, 4, and 5 in the rat liver, pancreas, and gut. Gastroenterology 2001; 120(2): 411-22.
  • 7. Burek M, Forster CY. Cloning and characterization of the murine claudin-5 promoter. Mol Cell Endocrinol 2009; 298(1-2): 19-24.
  • 8. Boivin MA, Ye D, Kennedy JC, Al-Sadi R, Shepela C, Ma TY. Mechanism of glucocorticoid regulation of the intestinal tight junction barrier. Am J Physiol-Gastr L 2007; 292(2): G590-G98.
  • 9. Peixoto EBMI, Collares-Buzato CB. Modulation of the epithelial barrier by dexamethasone and prolactin in cultured Madin-Darby canine kidney (MDCK) cells. Cell Biol Int 2006; 30(2): 101-13.
  • 10. Romero IA, Radewicz K, Jubin E, Michel CC, Greenwood J, Couraud PO, Adamson P. Changes in cytoskeletal and tight junctional proteins correlate with decreased permeability induced by dexamethasone in cultured rat brain endothelial cells. Neurosci Lett 2003; 344(2): 112-16.
  • 11. Forster C, Silwedel C, Golenhofen N, Burek M, Kietz S, Mankertz J, Drenckhahn D. Occludin as direct target for glucocorticoid-induced improvement of blood-brain barrier properties in a murine in vitro system. J Physiol-London 2005; 565(2): 475-86.
  • 12. Forster C, Burek M, Romero IA, Weksler B, Couraud PO, Drenckbahni D. Differential effects of hydrocortisone and TNF alpha on tight junction proteins in an in vitro model of the human blood-brain barrier. J Physiol-London 2008; 586(7): 1937-49.
  • 13. Stelwagen K, McFadden HA, Demmer J. Prolactin, alone or in combination with glucocorticoids, enhances tight junction formation and expression of the tight junction protein occludin in mammary cells. Mol Cell Endocrinol 1999; 156(1-2): 55-61.
  • 14. Felinski EA, Antonetti DA. Glucocorticoid regulation of endothelial cell tight junction gene expression: novel treatments for diabetic retinopathy Curr Eye Res 2005; 30(11): 949-57.
  • 15. Wild GE, Waschke KA, Bitton A, Thomson ABR. The mechanisms of prednisone inhibition of inflammation in Crohn's disease involve changes in intestinal permeability, mucosal TNF alpha production and nuclear factor kappa B expression. Aliment Pharm Therap 2003; 18(3): 309-17.
  • 16. Rutgeerts PJ. Conventional treatment of Crohn's disease: Objectives and outcomes. Inflamm Bowel Dis 2001; 7(1): S2-S8.
  • 17. Hoheisel D, Nitz T, Franke H, Wegener J, Hakvoort A, Tilling T, Galla HJ. Hydrocortisone reinforces the blood-brain barrier properties in a serum free cell culture system. Biochem Biophys Res Commun 1998; 244(1): 312-6.
  • 18. Antonetti DA, Wolpert EB, DeMaio L, Harhaj NS, Scaduto RC. Hydrocortisone decreases retinal endothelial cell water and solute flux coincident with increased content and decreased phosphorylation of occludin. J Neurochem 2002; 80(4): 667-77.
  • 19. Black BL. Development of glucose active-transport in embryonic chick intestine - influence of thyroxine and hydrocortisone. Comp Biochem Phys A 1988; 90(3): 379-86.
  • 20.Ozden O, Black BL, Ashwell CM, Tipsmark CK, Borski RJ, Grubb BJ. Developmental profile of claudin-3,-5, and-16 proteins in the epithelium of chick intestine. Anat Rec 2010; 293(7): 1175-83.
  • 21. Buckingham JC. Glucocorticoids: Exemplars of multi-tasking. Brit J Pharmacol 2006; 147(1): S258-68.

The Effects of Dexamethasone on Claudin Tight Junction proteins in 18-day Embryonic Chick Intestine

Year 2018, Volume: 15 Issue: 1, 6 - 10, 15.04.2018

Abstract

Claudin (cldn) protein family is the main component
of tight junction (TJ) strands and it is directly involved in the barrier function
of TJs. In addition to the contributing to the barrier function of TJs in
mature tissues, these junctions are also present and required for normal
embryonic development of epithelial sheets. Glucocorticoids (GC) are essential
for the maturation of numerous tissues including the intestine during embryonic
development. They also have stimulatory effects on the integrity of tight
junctions. In this study, the effect of a synthetic glucocorticoid,
dexamethasone (DEX) on mRNA expression of tight junctional proteins was
investigated in 18-day embryonic chick intestine in vitro using quantitative
Real-Time PCR (RT-PCR) analyses. Pre-hatched chick intestine will provide a
convenient model because the organization of the barrier is not completely
established, and it is relatively easy to maintain its morphological integrity
for at least 24 hours in an organ culture system. RT-PCR analyses revealed that
both claudin-3 (cldn-3) and claudin-5 (cldn-5) tight junction gene expressions
were induced in response to DEX in a dose dependent manner. 

References

  • 1. Black BL, Smith JE. Regulation of goblet cell-differentiation by calcium in embryonic chick intestine. Faseb J 1989; 3(14): 2653-9.
  • 2. Uni Z, Tako E, Gal-Garber O, Sklan D. Morphological, molecular, and functional changes in the chicken small intestine of the late-term embryo. Poultry Sci 2003; 82(11): 1747-54.
  • 3. Sonoda N, Furuse M, Sasaki H, Yonemura S, Katahira J, Horiguchi Y, Tsukita S. Clostridium perfringens enterotoxin fragment removes specific claudins from tight junction strands: evidence for direct involvement of claudins in tight junction barrier. J Cell Biol 1999; 147(1): 195-204.
  • 4. Van Itallie CM, Anderson JM. Claudins and epithelial paracellular transport. Annu Rev Physiol 2006; 68: 403-29.
  • 5. Colegio OR, Van Itallie CM, McCrea HJ, Rahner C, Anderson JM. Claudins create charge-selective channels in the paracellular pathway between epithelial cells. Am J Physiol-Cell Ph 2002; 283(1): C142-C7.
  • 6. Rahner C, Mitic LL, Anderson JM. Heterogeneity in expression and subcellular localization of claudins 2, 3, 4, and 5 in the rat liver, pancreas, and gut. Gastroenterology 2001; 120(2): 411-22.
  • 7. Burek M, Forster CY. Cloning and characterization of the murine claudin-5 promoter. Mol Cell Endocrinol 2009; 298(1-2): 19-24.
  • 8. Boivin MA, Ye D, Kennedy JC, Al-Sadi R, Shepela C, Ma TY. Mechanism of glucocorticoid regulation of the intestinal tight junction barrier. Am J Physiol-Gastr L 2007; 292(2): G590-G98.
  • 9. Peixoto EBMI, Collares-Buzato CB. Modulation of the epithelial barrier by dexamethasone and prolactin in cultured Madin-Darby canine kidney (MDCK) cells. Cell Biol Int 2006; 30(2): 101-13.
  • 10. Romero IA, Radewicz K, Jubin E, Michel CC, Greenwood J, Couraud PO, Adamson P. Changes in cytoskeletal and tight junctional proteins correlate with decreased permeability induced by dexamethasone in cultured rat brain endothelial cells. Neurosci Lett 2003; 344(2): 112-16.
  • 11. Forster C, Silwedel C, Golenhofen N, Burek M, Kietz S, Mankertz J, Drenckhahn D. Occludin as direct target for glucocorticoid-induced improvement of blood-brain barrier properties in a murine in vitro system. J Physiol-London 2005; 565(2): 475-86.
  • 12. Forster C, Burek M, Romero IA, Weksler B, Couraud PO, Drenckbahni D. Differential effects of hydrocortisone and TNF alpha on tight junction proteins in an in vitro model of the human blood-brain barrier. J Physiol-London 2008; 586(7): 1937-49.
  • 13. Stelwagen K, McFadden HA, Demmer J. Prolactin, alone or in combination with glucocorticoids, enhances tight junction formation and expression of the tight junction protein occludin in mammary cells. Mol Cell Endocrinol 1999; 156(1-2): 55-61.
  • 14. Felinski EA, Antonetti DA. Glucocorticoid regulation of endothelial cell tight junction gene expression: novel treatments for diabetic retinopathy Curr Eye Res 2005; 30(11): 949-57.
  • 15. Wild GE, Waschke KA, Bitton A, Thomson ABR. The mechanisms of prednisone inhibition of inflammation in Crohn's disease involve changes in intestinal permeability, mucosal TNF alpha production and nuclear factor kappa B expression. Aliment Pharm Therap 2003; 18(3): 309-17.
  • 16. Rutgeerts PJ. Conventional treatment of Crohn's disease: Objectives and outcomes. Inflamm Bowel Dis 2001; 7(1): S2-S8.
  • 17. Hoheisel D, Nitz T, Franke H, Wegener J, Hakvoort A, Tilling T, Galla HJ. Hydrocortisone reinforces the blood-brain barrier properties in a serum free cell culture system. Biochem Biophys Res Commun 1998; 244(1): 312-6.
  • 18. Antonetti DA, Wolpert EB, DeMaio L, Harhaj NS, Scaduto RC. Hydrocortisone decreases retinal endothelial cell water and solute flux coincident with increased content and decreased phosphorylation of occludin. J Neurochem 2002; 80(4): 667-77.
  • 19. Black BL. Development of glucose active-transport in embryonic chick intestine - influence of thyroxine and hydrocortisone. Comp Biochem Phys A 1988; 90(3): 379-86.
  • 20.Ozden O, Black BL, Ashwell CM, Tipsmark CK, Borski RJ, Grubb BJ. Developmental profile of claudin-3,-5, and-16 proteins in the epithelium of chick intestine. Anat Rec 2010; 293(7): 1175-83.
  • 21. Buckingham JC. Glucocorticoids: Exemplars of multi-tasking. Brit J Pharmacol 2006; 147(1): S258-68.
There are 21 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Özkan Özden

Publication Date April 15, 2018
Submission Date June 14, 2016
Acceptance Date February 28, 2017
Published in Issue Year 2018 Volume: 15 Issue: 1

Cite

APA Özden, Ö. (2018). The Effects of Dexamethasone on Claudin Tight Junction proteins in 18-day Embryonic Chick Intestine. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 15(1), 6-10.
AMA Özden Ö. The Effects of Dexamethasone on Claudin Tight Junction proteins in 18-day Embryonic Chick Intestine. Erciyes Üniv Vet Fak Derg. April 2018;15(1):6-10.
Chicago Özden, Özkan. “The Effects of Dexamethasone on Claudin Tight Junction Proteins in 18-Day Embryonic Chick Intestine”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 15, no. 1 (April 2018): 6-10.
EndNote Özden Ö (April 1, 2018) The Effects of Dexamethasone on Claudin Tight Junction proteins in 18-day Embryonic Chick Intestine. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 15 1 6–10.
IEEE Ö. Özden, “The Effects of Dexamethasone on Claudin Tight Junction proteins in 18-day Embryonic Chick Intestine”, Erciyes Üniv Vet Fak Derg, vol. 15, no. 1, pp. 6–10, 2018.
ISNAD Özden, Özkan. “The Effects of Dexamethasone on Claudin Tight Junction Proteins in 18-Day Embryonic Chick Intestine”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 15/1 (April 2018), 6-10.
JAMA Özden Ö. The Effects of Dexamethasone on Claudin Tight Junction proteins in 18-day Embryonic Chick Intestine. Erciyes Üniv Vet Fak Derg. 2018;15:6–10.
MLA Özden, Özkan. “The Effects of Dexamethasone on Claudin Tight Junction Proteins in 18-Day Embryonic Chick Intestine”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, vol. 15, no. 1, 2018, pp. 6-10.
Vancouver Özden Ö. The Effects of Dexamethasone on Claudin Tight Junction proteins in 18-day Embryonic Chick Intestine. Erciyes Üniv Vet Fak Derg. 2018;15(1):6-10.