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Düşük Doz Capsaicin Uygulanan Sıçanların Ovaryumlarında TGF-Beta 1’in İmmunohistokimyasal Yerleşimi

Year 2018, Volume: 15 Issue: 3, 238 - 246, 04.12.2018
https://doi.org/10.32707/ercivet.477313

Abstract

Transforme edici büyüme faktörü-beta1 (TGF-β1), ovaryum fonksiyonlarının düzenlenmesinde önemli rol oyna-maktadır. Sunulan çalışmada, sıçan ovaryumlarında düşük doz capsaicin (CAP) uygulamasının, follikülogenezis ve atrezi sürecinde TGF-β1 ekspresyonuna etkisinin olup/olmadığı incelendi. Otuz adet 21 günlük dişi Sprague-Dawley sıçan üç eşit grubu ayrıldı. İlk gruba hiçbir enjeksiyon yapılmadı (kontrol A grubu), ikinci gruba (deney grubu) taşıyıcı solüsyon içinde eritilen CAP (0.5 mg/kg/gün) enjeksiyonu yapıldı, üçüncü gruba (kontrol B grubu) sadece taşıyıcı solüs-yon enjeksiyonu yapıldı. Onbeş günlük uygulama periyodundan sonra hayvanlar eter inhalasyonu ile uyutularak ovar-yumları alındı ve rutin histolojik prosedür uygulandı. TGF-β1, işaretli streptavidin-biotin tekniği kullanılarak gösterildi. Deney grubunda oositler, tüm folliküllerin (primordiyal, primer, sekonder, preantral, antral folliküller) granuloza hücrele-ri, teka hücreleri ve intersitisyel hücreler pozitif boyandı. Kontrol grubundaki boyanmada genel olarak deney grubuna benzerdi, fakat preantral ve antral folliküllerin granuloza hücrelerinde boyanma yoktu. Deney grubundaki TGF-β1 bo-yanması, kontrol gruplarından daha yoğundu. Deney ve kontrol gruplarında atretik folliküllerde TGF-β1 ekspresyonu gözlenmedi. Sonuç olarak, CAP, follikülogenezis sırasında ovaryan hücrelerdeki kendi reseptörüne bağlanarak TGF-β1 ekspresyonunu arttırmıştır.

References

  • 1. Alatriste V, Herrera-Camacho I, Martinez MI, Limon ID, Gonzalez-Flores O, Luna F. Sensory denervation with capsaicin reduces ovarian follicular development and delays the onset of puberty in guinea pigs. Adv Reprod Sci 2013; 1(3): 29-37. 2. Bondestam J, Huotari MA, Morén A, Ustinov J, Kaivo-Oja N, Kallio J, Horelli-Kuitunen N, Aaltonen J, Fujii M, Moustakas A, Ten Dijke P, Otonkoski T, Ritvos O. cDNA cloning, expression studies and chromosome mapping of human type I serine/threonine kinase receptor ALK7 (ACVR1C). Cytogenet Cell Genet 2001; 95(3-4): 157-62. 3. Calka J, McDonald JK, Ojeda SR. The innervation of the immature rat ovary by calcitonin gene-related peptide. Biol Reprod 1988; 39(5): 1215-23. 4. Chang WY, Ohmura H, Kulp SK, Lin YC. Transforming growth factor β1 regulates differentiation of porcine granulosa cells in vitro. Theriogenology 1993; 40(4): 699-712. 5. Chegini N, Flanders KC. Presence of transforming growth factor-beta and their selective cellular localization in human ovarian tissue of various reproductive stages. Endocrinology 1992; 130(3): 1707-15. 6. Christopher B. Immunolocalization of transforming growth factor-beta1 during follicular development and atresia in the mouse ovary. Endocr J 2000; 47(4): 475-80. 7. Crossmonn G. A modification of mallory’s connective tissue stain with a discussion of the principles involved. Anat Rec 1937; 69(1): 33-8. 8. Derry S, Lloyd R, Moore RA, McQuay HJ. Topical capsaicin for chronic neuropathic pain in adults. Cochrane Database Syst Rev 2009; CD007393. 9. Dorrington J, Chuma AV, Bendell JJ. Transforming growth factor β and follicle-stimulating hormone promote rat granulosa cell proliferation. Endocrinology 1988; 123(1): 353-9. 10. Erdost H, Özer A,Yakışık M, Özfiliz N, Zık B. FSH and LH cells in the laying hens and cocks, fed with a diet containing red hot pepper. J Food Agri Environ 2006; 4(1): 119-23. 11. Filipczak-Bryniarska I, Krzyzewski RM, Kucharz J, Michalowska-Kaczmarczyk A, Kleja J, Woron J, Strzepek K, Kazior L, Wordliczek J, Grodzicki T, Krzemieniecki K. High-dose 8% capsaicin patch in treatment of chemotherapy-induced peripheral neuropathy: Single-center experience. Med Oncol 2017; DOI 10.1007/s12032-017-1015-1. 12. Gitay-Goran H, Kim IC, Miggans ST, Schomberg DW. Transforming growth factor beta modulates gonadotropin receptor expression in porcine and rat granulosa cells differently. Biol Reprod 1993; 48(6): 1284-9. 13. Güler S, Zık B. Effects Of Capsaicin on Ovarian Granulosa Cell Proliferation and Apoptosis. Cell Tissue Res 2018; DOI:10.1007/s00441-018-2803-4. 14. Hu PP, Datto MB, Wang XF. Molecular mechanisms of transforming growth factor-beta signaling. Endocr Rev 1998; 19(3): 349-63. 15. Jensen-Jaorlim E, Gajdzik L, Haberl I. Hot spices influence permeability of human intestinal epithelial monolayers. J Nutr 1998; 128(3): 577-81. 16. Juneja SC, Chegini N, Williams RS, Ksander GA. Ovarian intra-bursal administration of transforming growth factor β1 inhibits follicle rupture in gonadotropin-primed mice. Biol Reprod 1996; 55(6): 1444-51. 17. Knight PG, Glister C. Local roles of TGF- superfamily members in the control of ovarian follicle development. Anim Reprod Sci 2003; 78(3-4): 165-83. 18. Kohan-Ivani K, Gabler F, Selman A, Vega M, Romero C. Role of dihydrotestosterone (DHT) on TGF-β1 signaling pathway in epithelial ovarian cancer cells. J Cancer Res Clin Oncol 2016; 142(1): 47-58. 19. Kress M, Gutimann C, Averbeck B, Reeh PW. Calcitonin gene related peptid and prostaglandin E2 but not substance P release induced by antidromic nerve stimulation from rat skin in vitro. Neuroscience 1999; 89(1): 303-10. 20. Levacher C, Gautier C, Saez JM, Habert R. Immunohistochemical localization of transforming growth factor beta 1 and beta 2 in the fetal and neonatal rat ovary. Differentiation 1996; 61(1): 45-51. 21. Lin HY, Wang XF, Ng-Eaton E, Weinberg RA, Lodish HF. Expression cloning of the TGF-beta type II receptor, a functional transmembrane serine/threonine kinase. Cell 1992; 68(4): 775-85. 22. May JV, Stephenson LA, Turzcynski CJ, Fong HW, Mau YH, Davis JS. Transforming growth factor beta expression in the porcine ovary: evidence that theca cells are the major secretory source during antral follicle development. Biol Reprod 1996; 54(2): 485-96. 23. Mondschein JS, Canning SF, Hammond JM. Effects of transforming growth factor β on the production of immunoreactive insulin-like growth factor I and progesterone and on [3H] thymidine incorporation in porcine granulosa cell cultures. Endocrinology 1988; 123(4): 1970-6. 24. Moran C, Morales L, Razo SR, Apolonıo J, Quıroz U, Chavira R, Dominguez R. Effect of sensorial denervation induced by capsaicin injection at birth or on day three of life, on puberty, induced ovulation and pregnancy. Life Sci 2003; 73(16): 2113-25. 25. Nance DM, King TR, Nance PW. Neuroendocrine and behavioral effects of intrathecal capsaicin in adult female rats. Brain Res Bull 1987; 18(1): 109-14. 26. Nilsson EE, Doraiswamy V, Skinner MK. Transforming growth factor-beta isoform expression during bovine ovarian antral follicle development. Mol Reprod Dev 2003; 66(3): 237-46. 27. Ohtomo K, Ebihara N, Matsuda A, Tokura T, Funaki T, Murakami A. Role of TGF-β1 in tissue eosinophilia associated with vernal kerato-conjunctivitis. Exp Eye Res 2010; 91(5): 748-54. 28. Oliveros L, Forneris M, Aguado L. Secretion from neuropeptidetreated splenocytes modifies ovarian steroidogenesis. Medicina 2001; 61(1): 35-40. 29. Olson LM, Jones-Burton CM, Jablonka-Shariff A. Nitric oxide decreases estradiol synthesis of rats luteinized ovarian cells: possible role for nitric oxide in functional luteal regression. Endocrinology 1996; 137(8): 3531-9. 30. Özer A, Erdost H, Zık B. Histological investigations on the effects of feeding a diet containing red hot pepper on the reproductive organs of the chicken. Phytother Res 2005; 19(6): 501-5. 31. Özgüden Akkoc CG, Ozer A. Immunohistochemical localization of transforming growth factor β 1 and β 2 in mouse testes during postnatal development. Biotech Histochem 2011; 87(2): 154-9. 32. Panossian A, Gabrielian E, Wagner H. Dose-dependent reversal effects of Capsaicin on interleukin-1α production is associated with the metabolism of arachidonic acid (leukotriene B(4) and prostaglandin E(2)) as well as nitric oxide production in human leukocytes. Phytomedicine 1996; 3(2): 169-74. 33. Powell DW, Mifflin RC, Valentich JD, Crowe SE, Saade JI, West AB. Myofibroblasts, paracrine cells important in health and disease. Am J Physiol 1999; 277(1): C1-C19. 34. Raja-Khan N, Kunselman AR, Demers LM, Ewens KG, Spielman RS, Legros RS. A variant in the fibrillin-3 gene is associated with TGF-beta and inhibin B levels in women with polycystic ovary syndrome. Fertil Steril 2010; 94(7): 2916-9. 35. Roberts AJ. Skinner MK. Transforming growth factor-alpha and -beta differentially regulate growth and steroidogenesis of bovine thecal cells during antral follicle development. Endocrinology 1991; 129(4): 2041-8. 36. Roy SK, Ogren C, Roy C, Lu B. Cell-type-specific localization of transforming growth factor-beta 2 and transforming growth factor-beta 1 in the hamster ovary: differential regulation by follicle-stimulating hormone and luteinizing hormone. Biol Reprod 1992; 46(4): 595-606. 37. Siegel PM, Massagué J. Cytostatic and apoptotic actions of TGF-beta in homeostasis and cancer. Nat Rev Cancer 2003; 3(11): 807-21. 38. Skinner MK, Keski-Oja J, Osteen KG, Moses HL. Ovarian thecal cells produce transforming growth factor-beta which can regulate granulosa cell growth. Endocrinology 1987; 121(2): 786-92. 39. Smith MS, Freeman ME, Neill JD. The control of progesterone secretion during the cycle and early pseudopregnancy. Endocrinology 1975; 96(1): 219-26. 40. Surh YJ, Lee SS. Capsaicin in hot chili pepper: Carcinogen, co-carcinogen or anticarcinogen? Food Chem Toxicol 1996; 34(3): 313-16. 41. Teerds KJ, Dorrington JH. Immunohistochemical localization of transforming growth factor-beta 1 and -beta 2 during follicular development in the adult rat ovary. Mol Cell Endocrinol 1992; 84(1-2): 7-13. 42. True LD. Atlas of Diagnostic Immunohistopathology. First Edition. Philadelphia: Lippincott Williams & Wilkins, 1990; p.16-22. 43. Tütüncü Ş, Özfiliz N. Distribution of the vanilloid (capsaicin) receptor type 1 in the capsaicin treated rat ovaries on different sexual development periods. Rev Med Vet 2011; 162(10): 460-7. 44. Uzumcu M, Kuhn PE, Marano JE, Armenti AE, Passantino L. Early postnatal methoxychlor exposure inhibits folliculogenesis and stimulates anti-Mullerian hormone production in the rat ovary. J Endocrinol 2006; 191(3): 549-58. 45. Vaishnava P, Wang DH. Capsaicin sensitive sensory nerves and blood pressure regulation. Curr Med Chem Cardiovasc Hematol Agents 2003; 1(2): 177-88. 46. Wang F, Shi R, Zhao L. Expression and significance of TGF-β 1 and VEGF in formation of new blood vessels after rabbit corneal suture. Recent Adv Ophthalmol 2008; 28(2): 96-9. 47. Yang W, Gong X, Zhao X, An W, Wang X, Wang M. Capsaicin induces apoptosis in HeLa cells via Bax/Bcl-2 and Caspase-3 Pathways. Asian Pac J Trop Med 2006; 1(3-4): 1-7. 48. Yu SM, Lin KH. Morphological alterations in the trachea of capsaicin-pretreated rat during postnatal development. Zool Stud 2002; 41(1): 13-22. 49. Zık B, Özgüden Akkoç C.G. Tütüncü S, İlhan T, Yılmaztepe AO, Özenci CC. Effects of low dose capsaicin (CAP) on ovarian follicle development in prepubertal rat. Rev Med Vet 2010; 161(6): 288-94.
Year 2018, Volume: 15 Issue: 3, 238 - 246, 04.12.2018
https://doi.org/10.32707/ercivet.477313

Abstract

References

  • 1. Alatriste V, Herrera-Camacho I, Martinez MI, Limon ID, Gonzalez-Flores O, Luna F. Sensory denervation with capsaicin reduces ovarian follicular development and delays the onset of puberty in guinea pigs. Adv Reprod Sci 2013; 1(3): 29-37. 2. Bondestam J, Huotari MA, Morén A, Ustinov J, Kaivo-Oja N, Kallio J, Horelli-Kuitunen N, Aaltonen J, Fujii M, Moustakas A, Ten Dijke P, Otonkoski T, Ritvos O. cDNA cloning, expression studies and chromosome mapping of human type I serine/threonine kinase receptor ALK7 (ACVR1C). Cytogenet Cell Genet 2001; 95(3-4): 157-62. 3. Calka J, McDonald JK, Ojeda SR. The innervation of the immature rat ovary by calcitonin gene-related peptide. Biol Reprod 1988; 39(5): 1215-23. 4. Chang WY, Ohmura H, Kulp SK, Lin YC. Transforming growth factor β1 regulates differentiation of porcine granulosa cells in vitro. Theriogenology 1993; 40(4): 699-712. 5. Chegini N, Flanders KC. Presence of transforming growth factor-beta and their selective cellular localization in human ovarian tissue of various reproductive stages. Endocrinology 1992; 130(3): 1707-15. 6. Christopher B. Immunolocalization of transforming growth factor-beta1 during follicular development and atresia in the mouse ovary. Endocr J 2000; 47(4): 475-80. 7. Crossmonn G. A modification of mallory’s connective tissue stain with a discussion of the principles involved. Anat Rec 1937; 69(1): 33-8. 8. Derry S, Lloyd R, Moore RA, McQuay HJ. Topical capsaicin for chronic neuropathic pain in adults. Cochrane Database Syst Rev 2009; CD007393. 9. Dorrington J, Chuma AV, Bendell JJ. Transforming growth factor β and follicle-stimulating hormone promote rat granulosa cell proliferation. Endocrinology 1988; 123(1): 353-9. 10. Erdost H, Özer A,Yakışık M, Özfiliz N, Zık B. FSH and LH cells in the laying hens and cocks, fed with a diet containing red hot pepper. J Food Agri Environ 2006; 4(1): 119-23. 11. Filipczak-Bryniarska I, Krzyzewski RM, Kucharz J, Michalowska-Kaczmarczyk A, Kleja J, Woron J, Strzepek K, Kazior L, Wordliczek J, Grodzicki T, Krzemieniecki K. High-dose 8% capsaicin patch in treatment of chemotherapy-induced peripheral neuropathy: Single-center experience. Med Oncol 2017; DOI 10.1007/s12032-017-1015-1. 12. Gitay-Goran H, Kim IC, Miggans ST, Schomberg DW. Transforming growth factor beta modulates gonadotropin receptor expression in porcine and rat granulosa cells differently. Biol Reprod 1993; 48(6): 1284-9. 13. Güler S, Zık B. Effects Of Capsaicin on Ovarian Granulosa Cell Proliferation and Apoptosis. Cell Tissue Res 2018; DOI:10.1007/s00441-018-2803-4. 14. Hu PP, Datto MB, Wang XF. Molecular mechanisms of transforming growth factor-beta signaling. Endocr Rev 1998; 19(3): 349-63. 15. Jensen-Jaorlim E, Gajdzik L, Haberl I. Hot spices influence permeability of human intestinal epithelial monolayers. J Nutr 1998; 128(3): 577-81. 16. Juneja SC, Chegini N, Williams RS, Ksander GA. Ovarian intra-bursal administration of transforming growth factor β1 inhibits follicle rupture in gonadotropin-primed mice. Biol Reprod 1996; 55(6): 1444-51. 17. Knight PG, Glister C. Local roles of TGF- superfamily members in the control of ovarian follicle development. Anim Reprod Sci 2003; 78(3-4): 165-83. 18. Kohan-Ivani K, Gabler F, Selman A, Vega M, Romero C. Role of dihydrotestosterone (DHT) on TGF-β1 signaling pathway in epithelial ovarian cancer cells. J Cancer Res Clin Oncol 2016; 142(1): 47-58. 19. Kress M, Gutimann C, Averbeck B, Reeh PW. Calcitonin gene related peptid and prostaglandin E2 but not substance P release induced by antidromic nerve stimulation from rat skin in vitro. Neuroscience 1999; 89(1): 303-10. 20. Levacher C, Gautier C, Saez JM, Habert R. Immunohistochemical localization of transforming growth factor beta 1 and beta 2 in the fetal and neonatal rat ovary. Differentiation 1996; 61(1): 45-51. 21. Lin HY, Wang XF, Ng-Eaton E, Weinberg RA, Lodish HF. Expression cloning of the TGF-beta type II receptor, a functional transmembrane serine/threonine kinase. Cell 1992; 68(4): 775-85. 22. May JV, Stephenson LA, Turzcynski CJ, Fong HW, Mau YH, Davis JS. Transforming growth factor beta expression in the porcine ovary: evidence that theca cells are the major secretory source during antral follicle development. Biol Reprod 1996; 54(2): 485-96. 23. Mondschein JS, Canning SF, Hammond JM. Effects of transforming growth factor β on the production of immunoreactive insulin-like growth factor I and progesterone and on [3H] thymidine incorporation in porcine granulosa cell cultures. Endocrinology 1988; 123(4): 1970-6. 24. Moran C, Morales L, Razo SR, Apolonıo J, Quıroz U, Chavira R, Dominguez R. Effect of sensorial denervation induced by capsaicin injection at birth or on day three of life, on puberty, induced ovulation and pregnancy. Life Sci 2003; 73(16): 2113-25. 25. Nance DM, King TR, Nance PW. Neuroendocrine and behavioral effects of intrathecal capsaicin in adult female rats. Brain Res Bull 1987; 18(1): 109-14. 26. Nilsson EE, Doraiswamy V, Skinner MK. Transforming growth factor-beta isoform expression during bovine ovarian antral follicle development. Mol Reprod Dev 2003; 66(3): 237-46. 27. Ohtomo K, Ebihara N, Matsuda A, Tokura T, Funaki T, Murakami A. Role of TGF-β1 in tissue eosinophilia associated with vernal kerato-conjunctivitis. Exp Eye Res 2010; 91(5): 748-54. 28. Oliveros L, Forneris M, Aguado L. Secretion from neuropeptidetreated splenocytes modifies ovarian steroidogenesis. Medicina 2001; 61(1): 35-40. 29. Olson LM, Jones-Burton CM, Jablonka-Shariff A. Nitric oxide decreases estradiol synthesis of rats luteinized ovarian cells: possible role for nitric oxide in functional luteal regression. Endocrinology 1996; 137(8): 3531-9. 30. Özer A, Erdost H, Zık B. Histological investigations on the effects of feeding a diet containing red hot pepper on the reproductive organs of the chicken. Phytother Res 2005; 19(6): 501-5. 31. Özgüden Akkoc CG, Ozer A. Immunohistochemical localization of transforming growth factor β 1 and β 2 in mouse testes during postnatal development. Biotech Histochem 2011; 87(2): 154-9. 32. Panossian A, Gabrielian E, Wagner H. Dose-dependent reversal effects of Capsaicin on interleukin-1α production is associated with the metabolism of arachidonic acid (leukotriene B(4) and prostaglandin E(2)) as well as nitric oxide production in human leukocytes. Phytomedicine 1996; 3(2): 169-74. 33. Powell DW, Mifflin RC, Valentich JD, Crowe SE, Saade JI, West AB. Myofibroblasts, paracrine cells important in health and disease. Am J Physiol 1999; 277(1): C1-C19. 34. Raja-Khan N, Kunselman AR, Demers LM, Ewens KG, Spielman RS, Legros RS. A variant in the fibrillin-3 gene is associated with TGF-beta and inhibin B levels in women with polycystic ovary syndrome. Fertil Steril 2010; 94(7): 2916-9. 35. Roberts AJ. Skinner MK. Transforming growth factor-alpha and -beta differentially regulate growth and steroidogenesis of bovine thecal cells during antral follicle development. Endocrinology 1991; 129(4): 2041-8. 36. Roy SK, Ogren C, Roy C, Lu B. Cell-type-specific localization of transforming growth factor-beta 2 and transforming growth factor-beta 1 in the hamster ovary: differential regulation by follicle-stimulating hormone and luteinizing hormone. Biol Reprod 1992; 46(4): 595-606. 37. Siegel PM, Massagué J. Cytostatic and apoptotic actions of TGF-beta in homeostasis and cancer. Nat Rev Cancer 2003; 3(11): 807-21. 38. Skinner MK, Keski-Oja J, Osteen KG, Moses HL. Ovarian thecal cells produce transforming growth factor-beta which can regulate granulosa cell growth. Endocrinology 1987; 121(2): 786-92. 39. Smith MS, Freeman ME, Neill JD. The control of progesterone secretion during the cycle and early pseudopregnancy. Endocrinology 1975; 96(1): 219-26. 40. Surh YJ, Lee SS. Capsaicin in hot chili pepper: Carcinogen, co-carcinogen or anticarcinogen? Food Chem Toxicol 1996; 34(3): 313-16. 41. Teerds KJ, Dorrington JH. Immunohistochemical localization of transforming growth factor-beta 1 and -beta 2 during follicular development in the adult rat ovary. Mol Cell Endocrinol 1992; 84(1-2): 7-13. 42. True LD. Atlas of Diagnostic Immunohistopathology. First Edition. Philadelphia: Lippincott Williams & Wilkins, 1990; p.16-22. 43. Tütüncü Ş, Özfiliz N. Distribution of the vanilloid (capsaicin) receptor type 1 in the capsaicin treated rat ovaries on different sexual development periods. Rev Med Vet 2011; 162(10): 460-7. 44. Uzumcu M, Kuhn PE, Marano JE, Armenti AE, Passantino L. Early postnatal methoxychlor exposure inhibits folliculogenesis and stimulates anti-Mullerian hormone production in the rat ovary. J Endocrinol 2006; 191(3): 549-58. 45. Vaishnava P, Wang DH. Capsaicin sensitive sensory nerves and blood pressure regulation. Curr Med Chem Cardiovasc Hematol Agents 2003; 1(2): 177-88. 46. Wang F, Shi R, Zhao L. Expression and significance of TGF-β 1 and VEGF in formation of new blood vessels after rabbit corneal suture. Recent Adv Ophthalmol 2008; 28(2): 96-9. 47. Yang W, Gong X, Zhao X, An W, Wang X, Wang M. Capsaicin induces apoptosis in HeLa cells via Bax/Bcl-2 and Caspase-3 Pathways. Asian Pac J Trop Med 2006; 1(3-4): 1-7. 48. Yu SM, Lin KH. Morphological alterations in the trachea of capsaicin-pretreated rat during postnatal development. Zool Stud 2002; 41(1): 13-22. 49. Zık B, Özgüden Akkoç C.G. Tütüncü S, İlhan T, Yılmaztepe AO, Özenci CC. Effects of low dose capsaicin (CAP) on ovarian follicle development in prepubertal rat. Rev Med Vet 2010; 161(6): 288-94.
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Details

Primary Language Turkish
Journal Section Articles
Authors

Berrin Zık

Publication Date December 4, 2018
Submission Date November 14, 2017
Acceptance Date April 17, 2018
Published in Issue Year 2018 Volume: 15 Issue: 3

Cite

APA Zık, B. (2018). Düşük Doz Capsaicin Uygulanan Sıçanların Ovaryumlarında TGF-Beta 1’in İmmunohistokimyasal Yerleşimi. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 15(3), 238-246. https://doi.org/10.32707/ercivet.477313
AMA Zık B. Düşük Doz Capsaicin Uygulanan Sıçanların Ovaryumlarında TGF-Beta 1’in İmmunohistokimyasal Yerleşimi. Erciyes Üniv Vet Fak Derg. December 2018;15(3):238-246. doi:10.32707/ercivet.477313
Chicago Zık, Berrin. “Düşük Doz Capsaicin Uygulanan Sıçanların Ovaryumlarında TGF-Beta 1’in İmmunohistokimyasal Yerleşimi”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 15, no. 3 (December 2018): 238-46. https://doi.org/10.32707/ercivet.477313.
EndNote Zık B (December 1, 2018) Düşük Doz Capsaicin Uygulanan Sıçanların Ovaryumlarında TGF-Beta 1’in İmmunohistokimyasal Yerleşimi. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 15 3 238–246.
IEEE B. Zık, “Düşük Doz Capsaicin Uygulanan Sıçanların Ovaryumlarında TGF-Beta 1’in İmmunohistokimyasal Yerleşimi”, Erciyes Üniv Vet Fak Derg, vol. 15, no. 3, pp. 238–246, 2018, doi: 10.32707/ercivet.477313.
ISNAD Zık, Berrin. “Düşük Doz Capsaicin Uygulanan Sıçanların Ovaryumlarında TGF-Beta 1’in İmmunohistokimyasal Yerleşimi”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 15/3 (December 2018), 238-246. https://doi.org/10.32707/ercivet.477313.
JAMA Zık B. Düşük Doz Capsaicin Uygulanan Sıçanların Ovaryumlarında TGF-Beta 1’in İmmunohistokimyasal Yerleşimi. Erciyes Üniv Vet Fak Derg. 2018;15:238–246.
MLA Zık, Berrin. “Düşük Doz Capsaicin Uygulanan Sıçanların Ovaryumlarında TGF-Beta 1’in İmmunohistokimyasal Yerleşimi”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, vol. 15, no. 3, 2018, pp. 238-46, doi:10.32707/ercivet.477313.
Vancouver Zık B. Düşük Doz Capsaicin Uygulanan Sıçanların Ovaryumlarında TGF-Beta 1’in İmmunohistokimyasal Yerleşimi. Erciyes Üniv Vet Fak Derg. 2018;15(3):238-46.