Determination of Some Antibiotic Residues by HPLC Method in Chicken Meats Prepared for Consumption

Year 2018, Volume: 15 Issue: 3, 247 - 252, 04.12.2018

Emre Arslanbaş

https://doi.org/10.32707/ercivet.477315

Cited By: 1

Abstract

In this study, it is
aimed to search residues of enrofloxacin, doxycycline and tylosin which are
widely used in poultry farming in fresh and packaged chicken meat samples taken
from five brands being sold nationwide, through HPLC method. Enrofloxacin,
doxycycline and tylosin levels in the samples of chicken meat were evaluated
according to “The Regulation on Classification of Active Pharmacologic
Substances that might be Available in Food of Animal Origin in Turkish Food
Codex and Maximum Residue Limits” and international limits. Maximum residue
limit (MRL) of enrofloxacin, doxycycline and tylosin in muscle tissues of
chickens must be 100 µg/kg according to applicable regulations and
international limits. It was found in totally 300 analyzed samples (whole
chicken, drumstick and breast meat) that 11 (3.6%) of them had enrofloxacin,
doxycycline and tylosin levels higher than MRL (between 100-150 µg/kg). Residue
levels were less than allowed limits in other samples. Six (2%) of these 11
samples had enrofloxacin, 3 (1%) had doxycycline and 2 (0.6%) had tylosin
residues. Since the results of this study showed that the majority of samples were
lower than allowed limits, these results were considered as positive. However,
as long as medicines do continue to be used for animals’ health, there will
always be a risk of existence of medicine residues in food of animal origin.
Many stakeholders have a role in preventing residue risk arising out of
veterinary medicines and all these persons must pay necessary attention to
residue purification duration, training programs and keeping regular records of
animals.

Keywords

Chicken meat, doxycycline, enrofloxacin, residue, tylosin

References

• 1. Akar F. Investigation on various antibiotic residues in chicken meat and liver. AÜ Vet Fak Derg 1994; 41(2): 199-207. 2. Baydan E, Filazi A, Kum C, Sekkin S. Etlik piliçlerde pişirme ve dondurma işlemlerinin ilaç kalıntıları üzerine etkileri: 1. Enrofloksasin üzerine pişirme ve farklı sürelerde soğukta depolamanın etkisi. Türk Vet Hek Derg 2000; 71: 19-22. 3. Berrada H, Borrull F, Font G, Marcé RM. Determination of macrolide antibiotics in meat and fish using pressurized liquid extraction and liquid chromatography–mass spectrometry. J Chromatogr A 2008; 1208(2008): 83-9. 4. Berrada H, Moltó JC, Mañes J, Font G. Determination of aminoglycoside and macrolide antibiotics in meat by pressurized liquid extraction and LC-ESI-MS. J Sep Sci 2010; 33(4-5): 522-9. 5. Bousova K, Senyuva H, Mittendorf K. Quantitative multi-residue method for determination antibiotics in chicken meat using turbulent flow chromatography coupled to liquid chromatography-tandem mass spectrometry. J Chromatogr A 2013; 1274(2013): 19-27. 6. Can HY, Çelik TH. The residue risk and use of antibiotic in poultry breeding. Vet Hekim Der Derg 2008; 79(4): 35-40. 7. Cetinkaya F, Yibar A, Soyutemiz GE, Okutan B, Ozcan A, Karaca MY. Determination of tetracycline residues in chicken meat by liquid chromatography-tandem mass spectrometry. Food Addit Contam Part B 2012; 5(1): 45-9. 8. Chico J, Rubies A, Centrich F, Companyo R, Prat MD, Granados M. High-throughput multiclass method for antibiotic residue analysis by liquid chromatography–tandem mass spectrometry. J Chromatogr A 2008; 1213(2008): 189-99. 9. Commission Regulation. Commission Regulation (EU) No 37/2010 of 22 December 2009 on pharmacologically active substances and their classification regarding maximum residue limits in foodstuffs of animal origin. https://ec.europa.eu/health/sites/health/files/files/eudralex/vol-5/reg_2010_37/reg_2010_37_en.pdf. Erişim tarihi:02.10.2017. 10. Filazi A. Kanatlılarda akılcı antibakteriyel ilaç kullanımı. Mektup Ankara 2009; 7(2): 3-8. 11. Garcia-Ovando H, Gorla N, Weyers A, Ugnia L, Magnoli A. Simultenous quantification of ciprofloxacin, enrofloxacin and balofloxacin in broiler chicken muscle. Arch Med Vet 2004; 36(1): 93-8. 12. Kaya S, Şahal M. Drug residues in foodstuffs, their tolerance levels and withdrawal times for sloughtering animals or discarded times of milk from animals given drugs. AÜ Vet Fak Derg 1989; 36(2): 390-403. 13. Lee JB, Chung HH, Chung YH, Lee KG. Development of an analytical protocol for detecting antibiotic residues in various foods. Food Chem 2007; 105(2007): 1726-31. 14. Lopes RP, Reyes RC, Romero-González R, Frenich AG, Vidal JLM. Development and validation of a multiclass method for the determination of veterinary drug residues in chicken by ultra high performance liquid chromatography-tandem mass spectrometry. Talanta 2012; 89(2012): 201-8. 15. Öbekçi J. Tavuk eti ve karaciğerlerinde tetrasiklin grubu antibiyotiklerin HPLC ile saptanması. Uzmanlık Tezi. Tarım ve Köyişleri Bakanlığı. 2002. 16. Pena A, Silva LJG, Pereira A, Meisel L, Lino CM. Determination of fluoroquinolone residues in poultry muscle in Portugal. Anal Bioanal Chem 2010; 397(6): 2615-21. 17. Salehzadeh F, Salehzadeh A, Rokni N, Madani R, Golchinefar F. Enrofloxacin residue in chicken tissues from Tehran slaughterhouses in Iran. Pakistan J Nutrition 2007; 6(4): 409-13. 18. Sever E, Baydan E. The effect of various cooking and freezing processes on the levamisole residues in broiler tissues. Kafkas Univ Vet Fak Derg 2013; 19(2): 239-44. 19. Sırmagül B. Terapötik ilaç düzeyi izleminde analiz yöntemleri ve çalışma ilkeleri. Türk Farmakoloji Derneği XVII. Farmakoloji Eğitim Sempozyumu. 28 Mayıs 2010. Lefkoşa-Kıbrıs. 20. Tang HP, Ho C, Lai SS. High-throughput screening for multi-class veterinary drug residues in animal muscle using liquid chromatography/tandem mass spectrometry with on-line solid-phase extraction. Rapid Commun Mass Spectrom 2006; 20(17): 2565-72. 21. The Official Gazette of the Republic of Turkey. The regulation on classification of active pharmacologic substances that might be available in food of animal origin in Turkish food codex and maximum residue limits, T.C. Başbakanlık, Mevzuatı Geliştirme ve Yayın Genel Müdürlüğü Mevzuat Bilgi Sistemi, 04.05.2012, http://www.resmigazete.gov.tr/eskiler/2012/05/20120504-9.htm, Erişim tarihi: 11.10.2017. 22. Tuncer Hİ. To banned usage of hormones, antibiotics, anticoccidials and drugs in compound animal feed. Lalahan Hay Arast Enst Derg 2007; 47(1): 29-37. 23. Yıbar A, Soyutemiz GE. Antibiotics use in food-producing animals and possible residual risk. Atatürk Üniversitesi Vet Bil Derg 2013; 8(1): 97-104. 24. Yıldırım M, Demirel H, Bakırel T. The investigations on the residues of the some fluoroquinolone antibacterials in chicken meat marketed in Istanbul. İstanbul Üniv Vet Fak Derg 1996; 22(1): 63-7.