Prevalence and Serotype Distribution of Listeria monocytogenes Isolated from Retail Raw Meats

Year 2020, Volume: 17 Issue: 1, 22 - 27, 23.03.2020

Seyda Şahin Mahmut Niyazi Moğulkoç Recep Kalın

https://doi.org/10.32707/ercivet.690607

Abstract

Listeria monocytogenes is a foodborne pathogen, which has great importance for human and animal health. Although 13 serotypes have been identified for L. monocytogenes, four of these serotypes (1/2a, 1/2b, 1/2c and 4b) are significant with regard to human listeriosis. With this study, beef, sheep and chicken meat samples were examined for L. monocytogenes and serotype of isolates were detected by molecular methods. A total of 156 marketed meat samples comprising of beef, sheep and chicken were collected from different supermarkets, butchers and retail shops. L. monocytogenes contamination rate was found as 12.8% in overall meat samples. In this study L. monocytogenes were detected in meat samples of beef, sheep and chicken meat samples as 14.8% (8 of 54), 9.6% (5 of 52), 14% (7 of 50), respectively. Common serotype was belonged to 1/2c in beef meats, 1/2a in chicken meats. L. monocytogenes strains of sheep were serotyped first time and dominant serotype was found to be 1/2c in Turkey. The presence of L. monocytogenes, contamination level and predominant serotype distribution in diverse foods should be elucidated with comprehensive studies mainly in animal products. Additionally, genetic relationship of human and animal isolates should be revealed and the role of animal products in human listeriosis should be investigated.

Keywords

Listeria monocytogenes, mPCR, meat, serotype

Satışa Sunulan Etlerde Listeria monocytogenes Prevalansı ve Serotip Dağılımının Belirlenmesi

Abstract

Listeria monocytogenes, gıda kaynaklı patojenlerden biri olup insan ve hayvan sağlığı açısından büyük öneme sahiptir. L. monocytogenes’in 13 serotipi bulunmasına rağmen, bunlardan dördü (1/2a, 1/2b, 1/2c ve 4b) insan listeriozisi açısından önemlidir. Bu çalışmada sığır, koyun ve tavuk et örnekleri L. monocytogenes yönünden incelenerek, moleküler yöntemlerle serotipleri ortaya kondu. Sığır, koyun ve tavuk etinden oluşan toplam 156 örnek süpermarket, kasap ve perakende satış noktalarından alındı. Tüm et örneklerinin %12.8’i L. monocytogenes ile kontamine bulundu. Bu çalışmada, L. monocytogenes sığır et örneklerinde %14.8 (8/54), koyun et örneklerinde %9.6 (5/52), tavuk et örneklerinde %14.0 (7/50) oranında tespit edildi. Sığır etinde 1/2c, tavuk etinde ise 1/2a serotipi baskın olarak bulundu. Bu çalışmada, Türkiye’de koyun listeria suşları ilk defa serotiplendirildi ve baskın serotip 1/2c olarak belirlendi. Kapsamlı çalışmalar ile başta hayvansal gıdalar olmak üzere farklı kaynaklarda L. monocytogenes varlığı, kontaminasyon düzeyi ve predominant serotip dağılımı belirlenmelidir. Buna ilaveten, insan ve hayvan izolatlarının genetik ilişkileri ortaya konularak insan listeriozisinde hayvansal ürünlerin rolü araştırılmalıdır.

Keywords

Et, Listeria monocytogenes, mPCR, serotip

References

• Abay S, Aydın F, Sümerkan AB. Molecular typing of Listeria spp. isolated from different sources. Ankara Üniv Vet Fak Derg 2012; 59: 183-90.
• Ayaz ND, Ayaz Y, Kaplan YZ, Kasımoğlu Doğru A, Aksoy MH. Rapid detection of Listeria monocytogenes in chicken carcasses by IMS-PCR. Ann Microbiol 2009; 59(4): 741-44.
• Baek SY, Lim SY, Lee DH, Min KH, Kim CM. Incidence and characterization of Listeria monocytogenes from domestic and imported foods in Korea. J Food Protect 2000; 63(2): 186-89.
• Bouayad L, Hamdi TM, Naim M, Leclercq A, Lecuit M. Prevalence of Listeria spp. and molecular characterization of Listeria monocytogenes isolates from broilers at the abattoir. Foodborne Pathog Dis 2015; 12(7): 606-11.
• Carp-Carare C, Vlad-Sabie A, Floristean CV. Detection and serotyping of Listeria monocytogenes in some food products from North-East of Romania. Rev Romana Med Lab 2013; 21(3): 285-92.
• CDC. Surveillance System Overview: The Listeria Initiative. https://www.cdc.gov/listeria/pdf/listeriainitiativeoverview_508.pdf; Accessed date:15.11.2016.
• Cetinkaya F, Elal Mus T, Yibar A, Guclu N, Tavsanli H, Cibik R. Prevalence, serotype identification by multiplex polymerase chain reaction and antimicrobial resistance patterns of Listeria monocytogenes isolated from retail foods. J Food Safety 2014; 34: 42-9.
• D'Agostino M, Wagner M, Vazquez-Boland JA, Kuchta T, Karpiskova R, Hoorfar J, Cook N. A validated PCR-based method to detect Listeria monocytogenes using raw milk as a food model-towards an international standard. J Food Protect 2004; 67(8): 1646-55.
• Dogruer Y, Telli N, Telli AE, Güner A. Presence and antibiotic susceptibility of Listeria monocytogenes in retail meat and meat products. Int J Biol Res 2015; 3(2): 76-81.
• Doumith M, Buchrieser C, Glaser P, Jacquet C, Martin P. Differentiation of the major Listeria monocytogenes serovars by multiplex PCR. J Clin Microbiol 2004; 42(8): 3819-22.
• EFSA. The European Union Summary Report. Trends and Sources of Zoonoses, Zoonotic Agent and Food-borne Outbreaks in 2015. EFSA Journal. 14(12), 4634. https://www.efsa.europa.eu/en/efsajournal/pub/4634; Accessed Date: 16.07.2017.
• Erol İ, Şireli UT. Donmuş broiler karkaslarında Listeria monocytogenes’in varlığı ve serotip dağılımı. Turk J Vet Anim Sci 1999; 23(4): 765-70.
• Fallah AA, Saei-Dehkordi SS, Rahnama M, Tahmasby H, Mahzounieh MR. Prevalence and antimicrobial resistance patterns of Listeria species isolated from poultry products marketed in Iran. Food Control 2012; 28(2): 327-32.
• Guerra MM, McLauchin J, Bernardo FA. Listeria in ready-to-eat and unprocessed foods produced in Portugal. Food Microbiol 2001; 18(4): 423-29.
• Kahraman T, Büyükünal SK, Çetin O. Microbiological contamination of lamb carcasses at abattoirs of İstanbul. Vet Glasnik 2005; 59: 437-44.
• Kerouanton A, Marault M, Petit L, Grout J, Dao TT, Brisabois A. Evaluation of a multiplex PCR assay as an alternative method for Listeria monocytogenes serotyping. J Microbiol Methods 2010; 80(2): 134-7.
• Kocaman N, Sarımehmetoğlu B. Isolation of Listeria monocytogenes in lamb meat and determination of the antibiotic resistance. Ankara Üniv Vet Fak Derg 2017; 64: 273-79.
• Kramarenko T, Roasto M, Meremae K, Kuningas M, Poltsama P, Elias T. Listeria monocytogenes prevalence and serotype diversity in various foods. Food Control 2013; 30(1): 24-9.
• Mashak Z, Hamidreza AZ, Negin S, Afshin N, Basti A. Prevalence of Listeria monocytogenes in different kinds of meat in Tehran province, Iran. Brit Food J 2015; 117(1): 109-16.
• Mead GC. Microbiological quality of poultry meat: a review. Braz J Poult Sci 2004; 6(3): 135-42.
• Norton DW, Braden CR. Foodborne listeriosis. Ryser ET. Marth EH. eds. In: Listeria, Listeriosis and Food Safety. Boca Raton, New York, US: CRC Press, 2007; pp. 305-56.
• Ochiai Y, Yamada F, Batmunkh O, Mochizuki M, Takano T, Hondo R, Ueda F. Prevalence of Listeria monocytogenes in retailed meat in the Tokyo metropolitan area. J Food Protect 2010; 73(9): 1688-93.
• Pesavento G, Ducci B, Nieri D, Comodo N, Lo Nostro A. Prevalence and antibiotic susceptibility of Listeria spp. isolated from raw meat and retail foods. Food Control 2010; 21(5): 708-13.
• Praakle-Amin A, Hänninen ML, Korkeala H. Prevalence and genetic characterization of Listeria monocytogenes in retail broiler meat in Estonia. J Food Protect 2006; 69(2): 436-40.
• Schlech III WF. Foodborne listeriosis. Clin Infect Dis 2000; 31(3): 770-5.
• Shank FR, Elliot EL, Wachsmuth IK, Losikoff ME. US position on Listeria monocytogenes in foods. Food Control 1996; 7: 229-34.
• Siriken B, Ayaz ND, Erol I. Listeria monocytogenes in retailed raw chicken meat in Turkey. Berl Munch Tierarztl Wochenschr 2014; 127(1-2): 43-9.
• Tekay F, Özbek E, Kazancı E, Okur N, Demirel M, İpek MŞ. A case of neonatal sepsis due to Listeria monocytogenes serotype 4b: case report and literature review. Dicle Med J 2014; 41: 599-02.
• Turkish Food Codex (TFC) 2011. Microbiological Criteria Regulation. Republic of Turkey Ministry of Food, Agriculture and Livestock. Official Gazette: 29.12.2011. Issue: 28157, Ankara.
• Van Nierop W, Dusé AG, Marais E, Aithma N, Thothobolo N, Kassel M, Stewart R, Potgieter A, Fernandes B, Galpin JS, Bloomfield SF. Contamination of chicken carcasses in Gauteng, South Africa, by Salmonella, Listeria monocytogenes and Campylobacter. Int J Food Microbiol 2005; 99(1): 1-6.
• Vinay Kumar BN, Fairoza N, Madhavaprasad CB, Karabasanavar N, Kotresh AM, Nadoor P, Prashant S, Shilpa AG, Barbuddhe SB, Kurkure N, Chaudhary S. Molecular characterization and antibiogram of Listeria monocytogenes isolated from chicken and mutton of retail markets. J Microbiol Inf Dis 2016; 6(2); 65-8.
• Zhang Y, Yeh E, Hall G, Cripe J, Bhagwat AA, Meng J.Characterization of Listeria monocytogenes isolated from retail foods. Int J Food Microbiol 2007; 113(1): 47-3.