Toxoplasma gondii'li koyunlarda Oksidatif Stresin Glutatyon, Malondialdehit Düzeyi, Oksidatif Stres İndeksi, Toplam Oksidan Durumu ve Toplam Antioksidan Kapasitesi Kullanılarak Değerlendirilmesi

Year 2022, Volume: 19 Issue: 3, 182 - 188, 01.12.2022

Nergis Ulaş Mustafa Sinan Aktaş Kerim Emre Yanar Ömer Aydın Muhammed Sertaç Eroğlu Emre Eren

https://doi.org/10.32707/ercivet.1204263

Abstract

Bu çalışma, Toxoplasma gondii (T. gondii) ile doğal olarak enfekte olmuş koyunlarda malondialdehit (MDA), gluta- tyon (GSH), toplam oksidan durumu (TOS), toplam antioksidan (TAK) kapasite ve oksidatif stres indeksi (OSI) gibi belirteçler kullanılarak oksidatif stresi değerlendirmek amacıyla yapıldı. Bu çalışmada toplam 30 baş Morkaraman ırkı koyun kullanıldı. Kontrol için 10 sağlıklı koyundan ve T. gondiiile doğal olarak enfekte olmuş 20 koyundan kan örnekleri alındı. Serum örneklerinde MDA, GSH, TOS ve TAK seviyeleri belirlendi. Serum TOS’ un TAK seviyelerine oranı OSI olarak değerlendirildi. T. gondii ile doğal olarak enfekte olan koyunlarda serum MDA düzeyi önemli ölçüde artarken (P<0.01), serum TAK ve TOS düzeyleri önemli ölçüde azaldı (P<0.01). Serum GSH ve OSI düzeyleri yönünden gruplar arasında farklılık bulunamadı (P˃0.05). T. gondiiile doğal enfekte koyunlarda TAK ile OSI arasında negatif (P<0.01), TOS ile MDA arasında ise pozitif (P<0.01) korelasyon saptandı. Sonuç olarak T. gondiiile doğal enfekte koyunlarda OSI, MDA ve TAK değerlerinin oksidatif stres göstergesi olarak kullanılabileceği tespit edildi.

Keywords

Koyun, oksidatif stres, T. gondii

Evaluation of Oxidative Stress in Sheep with Toxoplasma gondii by Malondialdehyde, Glutathione Levels, Total Oxidant Status, Total Antioxidant Capacity and Oxidative Stress Index Markers

Abstract

This study was conducted to evaluate oxidative stress using markers such as malondialdehyde (MDA), glu- tathione (GSH), total oxidant status (TOS), total antioxidant capacity (TAC) and oxidative stress index (OSI) in sheep naturally infected with Toxoplasma gondii (T. gondii). A total of 30 Morkaraman breed sheep were used in this study. Blood samples were obtained from 10 healthy control sheep and 20 sheep naturally infected with T. gondii. malondial- dehyde, GSH, TOS and TAC levels were determined in serum samples. The ratio of serum TOS to TAC levels was assessed as OSI. While the serum MDA level increased significantly (P<0.01), the serum TAC and TOS levels de- creased significantly (P<0.01) in naturally infected with T. gondii sheep. There were no different for serum GSH and OSI in between the groups (P˃0.05). Negative correlation (P<0.01) between TAC and OSI and a positive correlation (P<0.01) between TOS and MDA were determined in naturally infected with T. gondii sheep. In conclusion, the results of study suggested that OSI, TAC and MDA could be used as indicator of oxidative stress for sheep naturally infected with T. gondii.

Keywords

Oxidative stress, sheep, T. gondii, Koyun, oksidatif stres, T. gondii

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