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Sıçanlarda Emamektin Benzoat Kaynaklı Oksidatif Hasara Karşı Diosminin Koruyucu Etkilerinin Araştırılması

Year 2023, Volume: 20 Issue: 2, 76 - 85, 01.08.2023
https://doi.org/10.32707/ercivet.1332103

Abstract

Bu çalışma ile sıçanlarda emamektin benzoat (EB) maruziyetine karşı diosminin (DİO) etkilerinin araştırılması amaçlandı. Çalışmada her grupta 10 tane olacak şekilde toplam 60 adet 6-8 haftalık 180-250 g ağırlığında Wistar Albi- no ırkı erkek sıçan kullanıldı. Gruplar sırasıyla kontrol, EB (10 mg kg-1), DİO (50 mg kg-1), DİO (100 mg kg-1), EB (10 mg kg-1) + DİO (50 mg kg-1), EB (10 mg kg-1) + DİO (100 mg kg-1) olarak belirlenerek belirtilen miktarlarda 21 gün boyunca gavajla uygulama yapıldı. Deneme süresinin sonunda anestezi altında kalbe punksiyon yapılarak heparinize ve antikoagulansız tüplere kan örneği alındı. Servikal dislokasyon sonrası sıçanların karaciğer, böbrek, beyin, testis, kalp ve akciğer dokuları çıkarıldı. Serumda bazı biyokimyasal parametreler (aspartat aminotransferaz (AST), alanin aminotransferaz (ALT), laktat dehidrogenaz (LDH), alkalin fosfataz (ALP), trigliserit, kolesterol, total protein, albümin, ürik asit, kan üre azotu (BUN) ve kreatinin) ölçülürken doku örneklerinde ise oksidatif stres parametreleri (MDA, NO, SOD, CAT, GSH-Px, GR, GST ve GSH) analiz edildi. Çalışma sonunda kontrol grubu ile karşılaştırıldığında, DİO uygu- lanan grubun parametrelerinde herhangi bir değişiklik tespit edilmedi. EMB uygulanan grupta kontrole kıyasla tüm dokularda MDA ile NO seviyelerinde anlamlı bir artış kaydedildi. GSH düzeyleri ile GSH, GR, GST, SOD ve CAT enzim aktivitelerinde ise azalma olduğu belirlendi. Ayrıca serum AST, ALT, ALP, LDH, kolesterol, trigliserit, BUN, ürik asit ve kreatinin düzey/aktivitelerinde artış görülürken serum total protein ve albümin düzeylerinde azalma görüldü. EB ile DİO’nun birlikte uygulandığı grupların değerlerinin doza bağlı olarak kısmen ya da tamamen kontrol grubunun değerlerine yaklaştığı tespit edildi. Sonuç olarak, DİO’nun sıçanlarda EMB kaynaklı toksisiteye karşı koruma sağlayabileceği düşünülmektedir.

References

  • Abdel-Daim MM, Abdellatief SA. Attenuating effects of caffeic acid phenethyl ester and betaine on abamectin-induced hepatotoxicity and nephrotoxi- city. Environ Sci Pollut Res Int 2018; 25(16): 15909-17.
  • Abdel-Daim MM, Khalifa HA, Abushouk AI, Dkhil MA, Al-Quraishy SA. Diosmin attenuates methotrexate -induced hepatic, renal, and cardiac injury: A bio- chemical and histopathological study in mice. Oxid Med Cell Longev 2017; 2017: 3281670. Abou-Zeid SM, AbuBakr HO, Mohamed MA, El- Bahrawy A. Ameliorative effect of pumpkin seed oil against emamectin induced toxicity in mice. Biomed Pharmacother 2018; 98: 242-51.
  • Ağır MS, Eraslan G. The effect of diosmin against liver damage caused by cadmium in rats. J Food Biochem 2019; 43(9): e12966.
  • Aktar MW, Sengupta D, Chowdhury A. Impact of pes- ticides use in agriculture: Their benefits and haz- ards. Interdiscip Toxicol 2009; 2(1): 1-12.
  • Bai SH, Ogbourne S. Eco-toxicological effects of the avermectin family with a focus on abamectin and ivermectin. Chemosphere 2016; 154: 204-14.
  • Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant de- fense. World Allergy Organ J 2012; 5(1): 9-19.
  • Bozdağ M, Eraslan G. The effect of diosmin against lead exposure in rats. Naunyn Schmiedebergs Arch Pharmacol 2020; 393(4): 639-49.
  • Carlberg I, Mannervik B. Glutathione reductase. Methods Enzymol 1985; 113: 484-90.
  • El-Sheikh el-SA, Galal AA. Toxic effects of sub- chronic exposure of male albino rats to emamectin benzoate and possible ameliorative role of Foeniculum vulgare essential oil. Environ Toxicol Pharmacol 2015; 39(3): 1177-88.
  • Eraslan G, Sarıca ZS, Bayram LÇ, Tekeli MY, Kanbur M, Karabacak M. The effects of diosmin on aflatoxin-induced liver and kidney damage. Environ Sci Pollut Res Int 2017; 24(36): 27931- 41.
  • Gerges SH, Wahdan SA, Elsherbiny DA, El- Demerdash E. Pharmacology of diosmin, a citrus flavone glycoside: an updated review. Eur J Drug Metab Pharmacokinet 2022; 47(1): 1-18.
  • Habig, WH, Pabst, MJ, Jakoby, WB. Glutahione Stransferase. The first enzymatic steps in mercapturic acid formation, J Biol Chem 1957; 249: 7130-9.
  • Hamed NA, Abdel-Razik RK. Biochemical alterations induced by abamectin in albino rats, Rattus norvegicus. Alex Sci Exch J 2015; 36: 287-73.
  • Jabłońska–Trypuć A, Wiater J. Protective effect of plant compounds in pesticides toxicity. J Environ Health Sci Engineer 2022; 20: 1035-45.
  • Jabłońska-Trypuć A. Pesticides as inducers of oxida- tive stress. React Oxyg Species 2017; 3(8): 96- 110.
  • Joint FAO/WHO Expert Committee on Food Additives (JECFA) (2013). Residue evaluation of certain veterinary drugs. JECFA Seventy Eighth Meeting, FAO JECFA Monographs 15. https:// www.fao.org/3/i3745e/i3745e.pdf; Accessed Date: 06.11.2021.
  • Khaldoun Oularbi H, Richeval C, Lebaili N, Zerrouki- Daoudi N, Baha M, Djennas N, Allorge D. Amelio- rative effect of vitamin C against hepatotoxicity induced by emamectin benzoate in rats. Hum Exp Toxicol 2017; 36(7): 709-17.
  • Lowry OH, Rosebrough NJ, Farr AL, Randal RJ. Pro- tein measurement with the folin phenol reagent. J Biol Chem 1951; 193: 265-75.
  • Luck H. Catalase. Bergmeyer HU. eds. In: Method of Enzymatic Analysis. New York: Academic Press, 1965; pp. 885-94.
  • Lü JM, Lin PH, Yao Q, Chen C. Chemical and molecular mechanisms of antioxidants: Experimental approaches and model systems. J Cell Mol Med 2010; 14(4): 840-60.
  • Madkour DA, Ahmed MM, Orabi SH, Sayed SM, Ko- rany RMS, Khalifa HK. Nigella sativa oil protects against emamectin benzoate-Induced neurotoxici- ty in rats. Environ Toxicol 2021; 36(8): 1521-35.
  • Meligi NM, Hassan HF. Protective effects of Eruca sativa (rocket) on abamectin insecticide toxicity in male albino rats. Environ Sci Pollut Res Int 2017; 24(10): 9702-12.
  • Mustafa S, Akbar M, Khan MA, Sunita K, Parveen S, Pawar JS, Massey S, Agarwal NR, Husain SA. Plant metabolite diosmin as the therapeutic agent in human diseases. Curr Res Pharmacol Drug Discov 2022; 3: 100122.
  • Nimse SB, Pal D. Free radicals, natural antioxidants, and their reaction mechanisms. RSC Adv 2015; 5 (35): 27986-8006.
  • Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95(2): 351-8.
  • Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70: 158-69.
  • Patel K, Gadewar M, Tahilyani V, Patel DK. A review on pharmacological and analytical aspects of di- osmetin: A concise report. Chin J Integr Med 2013; 19(10): 792-800.
  • Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, Squadrito F, Altavilla D, Bitto A. Oxi- dative stress: Harms and benefits for human health. Oxid Med Cell Longev 2017; 2017: 8416763.
  • Rehman MU, Tahir M, Quaiyoom Khan A, Khan R, Lateef A, Hamiza OO, Ali F, Sultana S. Diosmin protects against trichloroethylene-induced renal injury in Wistar rats: plausible role of p53, Bax and caspases. Br J Nutr 2013; 110(4): 699-710.
  • Sedlak J, Lindsay RH. Estimation of total, protein- bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem 1968; 25(1): 192-205.
  • Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 34(3): 497-500.
  • Tekeli MY, Eraslan G, Çakır Bayram L, Soyer Sarıca Z. Effect of diosmin on lipid peoxidation and organ damage against subacute deltamethrin exposure in rats. Environ Sci Pollut Res Int 2021; 28(13): 15890-908.
  • Tracey, WR, Tse J, Carter, G. Lipopolysaccharide- induced changes in plasma nitrite and nitrate concentrations in rats and mice: pharmacological evaluation of nitric oxide synthase inhibitors. J Pharmocol Ex Ther 1995; 272: 1011-5.
  • Zeng X, Du Z, Ding X, Jiang W. Protective effects of dietary flavonoids against pesticide-induced toxicity: A review. Trends Food Sci Technol 2021; 109: 271-9.
  • Zhang Y, Kong C, Chi H, Li J, Xing J, Wang F, Shao L, Zhai Q. Effect of a beta-cypermethrin and emamectin benzoate pesticide mixture on repro- ductive toxicity in male mice in a greenhouse en- vironment. Toxicol Mech Methods 2020; 30(2): 100-6.

Investigation of the Protective Effects of Diosmin Against Emamectin Benzoate Induced Oxidative Damage in Rats

Year 2023, Volume: 20 Issue: 2, 76 - 85, 01.08.2023
https://doi.org/10.32707/ercivet.1332103

Abstract

This study was aimed to investigate the effects of diosmin (DIO) in rats exposed to emamectin benzoate (EB). For this purpose, a total of 60 Wistar Albino male rats, aged 6 to 8 weeks and weighing 180 to 250 g, were used, 10 in each group. The groups were determined, respectively, control, EB (10 mg kg-1), DIO (50 mg kg-1), DIO (100 mg kg-1), EB (10 mg kg-1) + DIO (50 mg kg-1), EB (10 mg kg-1) + DIO (100 mg kg-1), and the indicated doses were applied by gavage for 21 days. At the end of the trial period, a heart puncture was performed under anesthesia and blood samples were taken into tubes with heparinized and anticoagulant-free properties. The liver, kidney, brain, testis, heart and lung tissues were removed after cervical dislocation. While some biochemical markers, such as aspartate aminotrans- ferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), triglyceride, cholesterol, total protein, albumin, uric acid, blood urea nitrogen (BUN) and, creatinine were assessed in the serum, oxidative stress indicators, such as MDA, NO, SOD, CAT, GSH-Px, GR, GST, and GSH, were determined in tissue samples. At the end of the investigation, no changes between the parameters of the DIO groups and the control group were discovered. Comparing the EB group to the control group, it was found that the levels of MDA and NO in tissues/ erythrocytes had significantly increased. The activity of the GSH, GR, GST, SOD, and CAT enzymes, as well as GSH levels, was found to have decreased. Furthermore, serum total protein and albumin levels were reduced, although AST, ALT, ALP, LDH, cholesterol, triglyceride, BUN, uric acid, and creatinine levels/activities were elevated. Depend- ing on the dose, it was found that the values of the groups coadministered with EMB and DIO were close to those of the control group. In conclusion, it is proposed that DIO could provide protection against EMB-induced toxicity in rats.

References

  • Abdel-Daim MM, Abdellatief SA. Attenuating effects of caffeic acid phenethyl ester and betaine on abamectin-induced hepatotoxicity and nephrotoxi- city. Environ Sci Pollut Res Int 2018; 25(16): 15909-17.
  • Abdel-Daim MM, Khalifa HA, Abushouk AI, Dkhil MA, Al-Quraishy SA. Diosmin attenuates methotrexate -induced hepatic, renal, and cardiac injury: A bio- chemical and histopathological study in mice. Oxid Med Cell Longev 2017; 2017: 3281670. Abou-Zeid SM, AbuBakr HO, Mohamed MA, El- Bahrawy A. Ameliorative effect of pumpkin seed oil against emamectin induced toxicity in mice. Biomed Pharmacother 2018; 98: 242-51.
  • Ağır MS, Eraslan G. The effect of diosmin against liver damage caused by cadmium in rats. J Food Biochem 2019; 43(9): e12966.
  • Aktar MW, Sengupta D, Chowdhury A. Impact of pes- ticides use in agriculture: Their benefits and haz- ards. Interdiscip Toxicol 2009; 2(1): 1-12.
  • Bai SH, Ogbourne S. Eco-toxicological effects of the avermectin family with a focus on abamectin and ivermectin. Chemosphere 2016; 154: 204-14.
  • Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant de- fense. World Allergy Organ J 2012; 5(1): 9-19.
  • Bozdağ M, Eraslan G. The effect of diosmin against lead exposure in rats. Naunyn Schmiedebergs Arch Pharmacol 2020; 393(4): 639-49.
  • Carlberg I, Mannervik B. Glutathione reductase. Methods Enzymol 1985; 113: 484-90.
  • El-Sheikh el-SA, Galal AA. Toxic effects of sub- chronic exposure of male albino rats to emamectin benzoate and possible ameliorative role of Foeniculum vulgare essential oil. Environ Toxicol Pharmacol 2015; 39(3): 1177-88.
  • Eraslan G, Sarıca ZS, Bayram LÇ, Tekeli MY, Kanbur M, Karabacak M. The effects of diosmin on aflatoxin-induced liver and kidney damage. Environ Sci Pollut Res Int 2017; 24(36): 27931- 41.
  • Gerges SH, Wahdan SA, Elsherbiny DA, El- Demerdash E. Pharmacology of diosmin, a citrus flavone glycoside: an updated review. Eur J Drug Metab Pharmacokinet 2022; 47(1): 1-18.
  • Habig, WH, Pabst, MJ, Jakoby, WB. Glutahione Stransferase. The first enzymatic steps in mercapturic acid formation, J Biol Chem 1957; 249: 7130-9.
  • Hamed NA, Abdel-Razik RK. Biochemical alterations induced by abamectin in albino rats, Rattus norvegicus. Alex Sci Exch J 2015; 36: 287-73.
  • Jabłońska–Trypuć A, Wiater J. Protective effect of plant compounds in pesticides toxicity. J Environ Health Sci Engineer 2022; 20: 1035-45.
  • Jabłońska-Trypuć A. Pesticides as inducers of oxida- tive stress. React Oxyg Species 2017; 3(8): 96- 110.
  • Joint FAO/WHO Expert Committee on Food Additives (JECFA) (2013). Residue evaluation of certain veterinary drugs. JECFA Seventy Eighth Meeting, FAO JECFA Monographs 15. https:// www.fao.org/3/i3745e/i3745e.pdf; Accessed Date: 06.11.2021.
  • Khaldoun Oularbi H, Richeval C, Lebaili N, Zerrouki- Daoudi N, Baha M, Djennas N, Allorge D. Amelio- rative effect of vitamin C against hepatotoxicity induced by emamectin benzoate in rats. Hum Exp Toxicol 2017; 36(7): 709-17.
  • Lowry OH, Rosebrough NJ, Farr AL, Randal RJ. Pro- tein measurement with the folin phenol reagent. J Biol Chem 1951; 193: 265-75.
  • Luck H. Catalase. Bergmeyer HU. eds. In: Method of Enzymatic Analysis. New York: Academic Press, 1965; pp. 885-94.
  • Lü JM, Lin PH, Yao Q, Chen C. Chemical and molecular mechanisms of antioxidants: Experimental approaches and model systems. J Cell Mol Med 2010; 14(4): 840-60.
  • Madkour DA, Ahmed MM, Orabi SH, Sayed SM, Ko- rany RMS, Khalifa HK. Nigella sativa oil protects against emamectin benzoate-Induced neurotoxici- ty in rats. Environ Toxicol 2021; 36(8): 1521-35.
  • Meligi NM, Hassan HF. Protective effects of Eruca sativa (rocket) on abamectin insecticide toxicity in male albino rats. Environ Sci Pollut Res Int 2017; 24(10): 9702-12.
  • Mustafa S, Akbar M, Khan MA, Sunita K, Parveen S, Pawar JS, Massey S, Agarwal NR, Husain SA. Plant metabolite diosmin as the therapeutic agent in human diseases. Curr Res Pharmacol Drug Discov 2022; 3: 100122.
  • Nimse SB, Pal D. Free radicals, natural antioxidants, and their reaction mechanisms. RSC Adv 2015; 5 (35): 27986-8006.
  • Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95(2): 351-8.
  • Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70: 158-69.
  • Patel K, Gadewar M, Tahilyani V, Patel DK. A review on pharmacological and analytical aspects of di- osmetin: A concise report. Chin J Integr Med 2013; 19(10): 792-800.
  • Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, Squadrito F, Altavilla D, Bitto A. Oxi- dative stress: Harms and benefits for human health. Oxid Med Cell Longev 2017; 2017: 8416763.
  • Rehman MU, Tahir M, Quaiyoom Khan A, Khan R, Lateef A, Hamiza OO, Ali F, Sultana S. Diosmin protects against trichloroethylene-induced renal injury in Wistar rats: plausible role of p53, Bax and caspases. Br J Nutr 2013; 110(4): 699-710.
  • Sedlak J, Lindsay RH. Estimation of total, protein- bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem 1968; 25(1): 192-205.
  • Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 34(3): 497-500.
  • Tekeli MY, Eraslan G, Çakır Bayram L, Soyer Sarıca Z. Effect of diosmin on lipid peoxidation and organ damage against subacute deltamethrin exposure in rats. Environ Sci Pollut Res Int 2021; 28(13): 15890-908.
  • Tracey, WR, Tse J, Carter, G. Lipopolysaccharide- induced changes in plasma nitrite and nitrate concentrations in rats and mice: pharmacological evaluation of nitric oxide synthase inhibitors. J Pharmocol Ex Ther 1995; 272: 1011-5.
  • Zeng X, Du Z, Ding X, Jiang W. Protective effects of dietary flavonoids against pesticide-induced toxicity: A review. Trends Food Sci Technol 2021; 109: 271-9.
  • Zhang Y, Kong C, Chi H, Li J, Xing J, Wang F, Shao L, Zhai Q. Effect of a beta-cypermethrin and emamectin benzoate pesticide mixture on repro- ductive toxicity in male mice in a greenhouse en- vironment. Toxicol Mech Methods 2020; 30(2): 100-6.
There are 35 citations in total.

Details

Primary Language English
Subjects Veterinary Pharmacology
Journal Section Articles
Authors

Muhammet Yasin Tekeli This is me 0000-0002-9382-9605

Publication Date August 1, 2023
Submission Date November 16, 2022
Acceptance Date March 17, 2023
Published in Issue Year 2023 Volume: 20 Issue: 2

Cite

APA Tekeli, M. Y. (2023). Investigation of the Protective Effects of Diosmin Against Emamectin Benzoate Induced Oxidative Damage in Rats. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 20(2), 76-85. https://doi.org/10.32707/ercivet.1332103
AMA Tekeli MY. Investigation of the Protective Effects of Diosmin Against Emamectin Benzoate Induced Oxidative Damage in Rats. Erciyes Üniv Vet Fak Derg. August 2023;20(2):76-85. doi:10.32707/ercivet.1332103
Chicago Tekeli, Muhammet Yasin. “Investigation of the Protective Effects of Diosmin Against Emamectin Benzoate Induced Oxidative Damage in Rats”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 20, no. 2 (August 2023): 76-85. https://doi.org/10.32707/ercivet.1332103.
EndNote Tekeli MY (August 1, 2023) Investigation of the Protective Effects of Diosmin Against Emamectin Benzoate Induced Oxidative Damage in Rats. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 20 2 76–85.
IEEE M. Y. Tekeli, “Investigation of the Protective Effects of Diosmin Against Emamectin Benzoate Induced Oxidative Damage in Rats”, Erciyes Üniv Vet Fak Derg, vol. 20, no. 2, pp. 76–85, 2023, doi: 10.32707/ercivet.1332103.
ISNAD Tekeli, Muhammet Yasin. “Investigation of the Protective Effects of Diosmin Against Emamectin Benzoate Induced Oxidative Damage in Rats”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 20/2 (August 2023), 76-85. https://doi.org/10.32707/ercivet.1332103.
JAMA Tekeli MY. Investigation of the Protective Effects of Diosmin Against Emamectin Benzoate Induced Oxidative Damage in Rats. Erciyes Üniv Vet Fak Derg. 2023;20:76–85.
MLA Tekeli, Muhammet Yasin. “Investigation of the Protective Effects of Diosmin Against Emamectin Benzoate Induced Oxidative Damage in Rats”. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, vol. 20, no. 2, 2023, pp. 76-85, doi:10.32707/ercivet.1332103.
Vancouver Tekeli MY. Investigation of the Protective Effects of Diosmin Against Emamectin Benzoate Induced Oxidative Damage in Rats. Erciyes Üniv Vet Fak Derg. 2023;20(2):76-85.