Production and Characterization of Bacteriocin-like Peptide Produced by Bacillus amyloliquefaciens B10

Year 2014, Volume: 30 Issue: 5, 338 - 345, 01.10.2014

Seyma Sensoy Karaoglu Ali Sevim Elif Sevim

Abstract

Bacillus amyloliquefaciens B10 that was isolated from soil was identified according to data of morphological, biochemical and 16S rRNA sequence analyses. The bacteriocin like peptide (BLP) produced by B. amyloliquefaciens B10 shows extendend antimicrobial activity against gram-positive bacteria including some pathogenic bacteria such as B. cereus and L. monocytogenesis and yeast including opportunistic pathogen such as Candida albicans. The BLP was sensitive to proteolytic enzyme proteinase K, lipase, tripsin, α- amylase except for catalase (5 mg/ml). While the activity of BLP of B13 was stable at 90°C for 30 min, the activity was completely lost at 90°C for 2 h and 121°C 20 min. Although activity of BLP of B10 was stable pH range 3-9, optimum pH was 7.0. It was sensitive against some organic solvent and detergent such as ethanol, hexane, ethyl ether and tween-80. Partial purification of BLP of B10 performed 70% ammonium sulphate precipitation was shown that it has approximately 5 kDa molecular weight.

Keywords

Bacillus amyloliquefaciens B10, bacteriocin like peptide, antimicrobial activity, partial purification

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