Sazan Balığının (Cyprinus carpio Linnaeus, 1758) gastrointestinal kanalın histokimyasal yapısı

Yıl 2013, Cilt: 29 Sayı: 2, 109 - 113, 01.04.2013

Nurgül Şenol Kenan Çınar Ülker Eren

Öz

Bu çalışmada sazan balığının (Cyprinus carpio) genişlemiş ön bağırsak bölgesi ile ilk, orta, son bağırsak bölgelerinin histokimyasal yöntemlerle mukosubstans özelliklerinin belirlenmesi amaçlanmaktadır. Sazanların genişlemiş ö n bağırsak bölgesi ile ilk, orta ve son bağırsaklarından alınan doku örnekleri Bouin tespit solusyonunda 16 – 18 saat tespit edildi. Örnekler daha sonra rutin doku takibinden geçirilip parafinde bloklandı. Parafin bloklardan 6 - 7  kalınlığında alınan kesitle re genel histolojik yapının belirlenmesi için hematoksilen - eosin boyama yöntemi uygulandı. Çalışılan bölgelerin gastrointestinal mukosubstans özelliklerinin belirlenmesi için histokimyasal boyama yöntemleri uygulandı. Genel olarak uygulanan histokimyasal b oyama yöntemleri incelendiğinde çalışılan tüm bölgelerde reaksiyon oluştuğu saptandı. AB pH 2.5, AF, PAS ve PAS/AB pH 2.5 uygulamalarının, diğer uygulamalara göre daha güçlü reaksiyon verdiği gözlendi. Mukosubstans genişlemiş ön bağırsak bölgesinden son ba ğırsağa doğru artış gösterdiği tespit edildi. Bağırsak bölümlerinde ise yoğunluğun özellikle son bağırsakta maksimum seviyede olduğu belirlendi. AB 0.06 M ve AB 0.3 M uygulamalarında ise oldukça zayıf reaksiyon gözlendi. Sazan balığının (Cyprinus carpio) ç alışılan bölgelerinin mukosubstans içeriğinin daha çok asidik karakterli olduğu belirlendi.

Anahtar Kelimeler

Sazan, Cyprinus carpio, Histokimya, Gastrointestinal kanal

Histochemical Structure of Gastrointestinal Tract of Carp (Cyprinus carpio)

Öz

In this study, it is aimed the determination of mucosubstance characteristic histochemical at the enlerged area anterior intestine, middle and posterior intestines of carp (Cyprinus carpio). Samples were taken from the enlerged area anterior intestine, mid dle and posterior intestine. All samples were fixed for 16 - 18 h in Bouin’s fluid. After dehydration by passing tissues through a series of alcohol solutions, the samples were vacuum embedded in paraffin. Sections (6 – 7  ) were stained for general morphologi cal purposes with haematoxylin and eosin (H and E) stains. Histochemical techniques were performed for the localization and differentiation of carbohydrate moieties In general, the histochemical staining methods applied to an examination of the reactions w as found in all regions. AB pH 2.5, AF, PAS and PAS/AB pH 2.5 applicatios was stronger reaction than other applications. Mucosubstances densities increased from the enlerged area anterior intestine towards the posterior intestine. The maximum level of int ensity posterior intestine was determined. AB 0.06 M ve AB 0.3 M applications quite weak reaction was observed. Mucosubstance content of carp (Cyprinus carpio) was determined to be more acidic character

Anahtar Kelimeler

Carp, Cyprinus carpio, Histochemical, Gastrointestinal tract

Kaynakça

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