İlaç Adayı olarak Yeni Sentezlenen Pirimidin- Triazole Yapılı Bir Bileşiğin Elektrokimyasal Yapısının İncelenmesi

Yıl 2023, Cilt: 39 Sayı: 3, 475 - 484, 31.12.2023

Fatma Kurul , Huseyin Istanbullu , Seda Nur Topkaya Çetin

Öz

Bu çalışmada, umut vadeden yeni bir ilaç adayı olarak triazolopirimidinone türevi olan 5-(klorometil)-2-(2-klorofenil)-[1,2,4]triazolo[1,5-a]pirimidin-7(3H)-on (kısaltılmış olarak CMCP) sentezlenmiştir. Diferansiyel Puls Voltametri (DPV) ve Döngüsel Voltametri (CV) gibi elektrokimyasal teknikler kullanılarak, elektrokimyasal özellikleri, ayrıca tek sarmal DNA (ssDNA) ve çift sarmal DNA (dsDNA) ile etkileşimleri araştırılmıştır. Optimum sonuçlar sağlamak için pH, konsantrasyon, tarama hızı ve immobilizasyon süresi gibi çeşitli deneysel parametreler araştırılmıştır. Çalışmada, ilaçla etkileşimden önce ve sonra guanin bazlarının pik akımı gibi elektrokimyasal sinyallerdeki değişiklikleri analiz edilmiştir. Ayrıca, CMCP üzerinde birkaç gün boyunca kararlılık testleri yapılmıştır. Sonuçlar, CMCP’nin hem ssDNA hem de dsDNA ile etkileşiminden sonra guanin bazlarında dikkat çekici değişiklikler olduğunu ortaya koymuştur ve bu bileşiğin DNA yapısı üzerindeki potansiyel etkisini vurgulamaktadır. Bu deneysel bulgular, CMCP’nin DNA üzerindeki etkisi nedeniyle ilerideki ilaç geliştirme çalışmaları için umut vadeden bir aday olduğu görüşünü desteklemektedir.

Anahtar Kelimeler

İlaç Adayı, İlaç-DNA Etkileşimi, Triazolopirimidinon, Kalem Grafit Elektrot

Kaynakça

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