Assessment of the Remineralization Effect of Hemp-Containing Toothpaste

Abstract

Objectives: The study aimed to evaluate the remineralizing effect of hemp on initial enamel caries lesions. Materials and method: Initial caries lesions were created on twenty-eight intact enamel samples with demineralization solution (pH=4.5) in a shaking incubator for 72 hours at 37⁰C. Samples were divided into 4 groups (n=7) ((1) experimental toothpaste containing hemp (H) (SPC Kozmetik), (2) hemp oil (HO), (3) Sensodyne Repair and Protection toothpaste (S) (GSK), (4) Control (no treatment)). The agents were applied using a pH-cycle model for seven days. All agents were kept on the surface of samples for 2 minutes and then applied for 1 minute using an electric toothbrush (Oral-B Test Drive (Genius)). Samples were evaluated with microhardness, FluoreCam, and DIAGNOdent Pen. Data were statistically analyzed with ANOVA and Kruskal Wallis H tests (p<0.05). Results: According to fluorescence and microhardness evaluations, the remineralization effect was similar in all treatment groups, except for hemp oil (p<0.01). Groups S (288.86±9.63), and H (284.86±10.41) showed significantly higher microhardness values compared to HO (238.57±10.89) (p<0.001). According to FluoreCam data, lesion size decreased in S (-1.83) and H (-1.07), while an increase was observed in HO (0.75). According to DIAGNOdent Pen data, the mineral increase in S (-8.42±1.18) and H (-8.29±1.03) was significantly higher than in HO (-3.57±2.06) (p<0.001). Conclusions: Herbal toothpaste containing hemp (H) demonstrated a similar remineralization effect to Fluor and Novamin-containing toothpaste (S).

Keywords

• initial caries lesion
• herbal toothpaste
• demineralization
• hemp
• remineralization

Kenevir İçeren Diş Macununun Remineralizasyon Etkisinin Değerlendirilmesi

Abstract

Amaç: Bu çalışma, kenevirin başlangıç mine çürük lezyonlarına remineralize edici etkisini değerlendirmeyi amaçlamıştır. Gereç ve Yöntemler: Yirmi sekiz sağlam mine numunesi üzerinde demineralizasyon solüsyonu (pH=4.5) kullanılarak çalkalamalı inkübatörde 37⁰C'de 72 saat süreyle başlangıç çürük lezyonları oluşturulmuştur. Mine örnekleri 4 gruba ayrılmıştır (n=7) ((1) kenevir içeren deneysel diş macunu (H) (SPC Kozmetik), (2) kenevir yağı (HO), (3) Sensodyne Onarım ve Koruma diş macunu (S) (GSK) ), (4) Kontrol (tedavi yok)). Ajanlar yedi gün boyunca pH döngüsü modeli kullanılarak uygulanmıştır. Remineralizasyon ajanları diş yüzeyinde 2 dakika bekletildikten sonra elektrikli diş fırçası (Oral-B Test Drive (Genius)) ile 1 dakika süreyle uygulanmıştır. Örnekler mikrosertlik, FluoreCam ve DIAGNOdent Pen ile değerlendirilmiştir. Veriler ANOVA ve Kruskal Wallis H testleri ile istatistiksel olarak analiz edilmiştir (p<0,05). Bulgular: Floresans ve mikrosertlik sonuçlarına göre kenevir yağı hariç tüm tedavi gruplarında benzer remineralizasyon etkisi görülmüştür (p<0.01). Grup S (288,86±9,63) ve H (284,86±10,41), HO'ya (238,57±10,89) kıyasla anlamlı derecede yüksek mikrosertlik değerleri göstermiştir (p<0,001). FluoreCam verilerine göre S (-1,83) ve H'de (-1,07) lezyon boyutunda azalma görülürken, HO'da (0,75) artış gözlenmiştir. DIAGNOdent Pen verilerine göre S (-8,42±1,18) ve H'deki (-8,29±1,03) mineral artışı HO'ya (-3,57±2,06) göre anlamlı derecede yüksekti (p<0,001). Sonuç: Kenevir içeren bitkisel diş macunu (H), flor ve Novamin içeren diş macununa (S) benzer remineralizasyon etkisi göstermiştir.

Keywords

• başlangıç çürük lezyonu
• bitkisel diş macunu
• demineralizasyon
• kenevir
• remineralizasyon

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