Kenevir İçeren Diş Macununun Remineralizasyon Etkisinin Değerlendirilmesi

Year 2023, Volume: 7 Issue: 3, 147 - 153, 26.12.2023

Elif Alkan , Dilek Tağtekin , Nesrin Korkmaz , Funda Yanıkoğlu

https://doi.org/10.29228/erd.59

Abstract

Amaç: Bu çalışma, kenevirin başlangıç mine çürük lezyonlarına remineralize edici etkisini değerlendirmeyi amaçlamıştır.
Gereç ve Yöntemler: Yirmi sekiz sağlam mine numunesi üzerinde demineralizasyon solüsyonu (pH=4.5) kullanılarak çalkalamalı inkübatörde 37⁰C'de 72 saat süreyle başlangıç çürük lezyonları oluşturulmuştur. Mine örnekleri 4 gruba ayrılmıştır (n=7) ((1) kenevir içeren deneysel diş macunu (H) (SPC Kozmetik), (2) kenevir yağı (HO), (3) Sensodyne Onarım ve Koruma diş macunu (S) (GSK) ), (4) Kontrol (tedavi yok)). Ajanlar yedi gün boyunca pH döngüsü modeli kullanılarak uygulanmıştır. Remineralizasyon ajanları diş yüzeyinde 2 dakika bekletildikten sonra elektrikli diş fırçası (Oral-B Test Drive (Genius)) ile 1 dakika süreyle uygulanmıştır. Örnekler mikrosertlik, FluoreCam ve DIAGNOdent Pen ile değerlendirilmiştir. Veriler ANOVA ve Kruskal Wallis H testleri ile istatistiksel olarak analiz edilmiştir (p<0,05).
Bulgular: Floresans ve mikrosertlik sonuçlarına göre kenevir yağı hariç tüm tedavi gruplarında benzer remineralizasyon etkisi görülmüştür (p<0.01). Grup S (288,86±9,63) ve H (284,86±10,41), HO'ya (238,57±10,89) kıyasla anlamlı derecede yüksek mikrosertlik değerleri göstermiştir (p<0,001). FluoreCam verilerine göre S (-1,83) ve H'de (-1,07) lezyon boyutunda azalma görülürken, HO'da (0,75) artış gözlenmiştir. DIAGNOdent Pen verilerine göre S (-8,42±1,18) ve H'deki (-8,29±1,03) mineral artışı HO'ya (-3,57±2,06) göre anlamlı derecede yüksekti (p<0,001).
Sonuç: Kenevir içeren bitkisel diş macunu (H), flor ve Novamin içeren diş macununa (S) benzer remineralizasyon etkisi göstermiştir.

Keywords

başlangıç çürük lezyonu, bitkisel diş macunu, demineralizasyon, kenevir, remineralizasyon

Project Number

This study was supported by the Marmara University Scientific Research Projects Commission with the project number TDH-2020-10157.

Assessment of the Remineralization Effect of Hemp-Containing Toothpaste

Abstract

Objectives: The study aimed to evaluate the remineralizing effect of hemp on initial enamel caries lesions.
Materials and method: Initial caries lesions were created on twenty-eight intact enamel samples with demineralization solution (pH=4.5) in a shaking incubator for 72 hours at 37⁰C. Samples were divided into 4 groups (n=7) ((1) experimental toothpaste containing hemp (H) (SPC Kozmetik), (2) hemp oil (HO), (3) Sensodyne Repair and Protection toothpaste (S) (GSK), (4) Control (no treatment)). The agents were applied using a pH-cycle model for seven days. All agents were kept on the surface of samples for 2 minutes and then applied for 1 minute using an electric toothbrush (Oral-B Test Drive (Genius)). Samples were evaluated with microhardness, FluoreCam, and DIAGNOdent Pen. Data were statistically analyzed with ANOVA and Kruskal Wallis H tests (p<0.05).
Results: According to fluorescence and microhardness evaluations, the remineralization effect was similar in all treatment groups, except for hemp oil (p<0.01). Groups S (288.86±9.63), and H (284.86±10.41) showed significantly higher microhardness values compared to HO (238.57±10.89) (p<0.001). According to FluoreCam data, lesion size decreased in S (-1.83) and H (-1.07), while an increase was observed in HO (0.75). According to DIAGNOdent Pen data, the mineral increase in S (-8.42±1.18) and H (-8.29±1.03) was significantly higher than in HO (-3.57±2.06) (p<0.001). Conclusions: Herbal toothpaste containing hemp (H) demonstrated a similar remineralization effect to Fluor and Novamin-containing toothpaste (S).

Keywords

initial caries lesion, herbal toothpaste, demineralization, hemp, remineralization

Ethical Statement

Ethical approval of the study was obtained from the Clinical Research Ethics Committee of Marmara University Faculty of Dentistry (Date: 03.09.2020 Decision no: 2020/59).

Supporting Institution

This study was supported by the Marmara University Scientific Research Projects Commission with the project number TDH-2020-10157.

Project Number

This study was supported by the Marmara University Scientific Research Projects Commission with the project number TDH-2020-10157.

Thanks

The authors thank Kağan Çelebi from SPC Kozmetik for the production of experimental toothpastes containing hemp.

References

• Ancuceanu R, Anghel AI, Ionescu C, Hovaneț MV, Cojocaru-Toma M, Dinu M. Clinical trials with herbal products for the prevention of dental caries and their quality. A scoping study. Biomolecules. 2019;9(12):884.
• Arifa MK, Ephraim R, Rajamani T. Recent advances in dental hard tissue remineralization. A review of literature. Int. J. Clin. Pediatr. Dent. 2019;12(2):139.
• Bellocchio L, Patano A, Inchingolo AD, Inchingolo F, Dipalma G, Isacco CG, De Ruvo E, Rapone B, Mancini A, Lorusso F. Cannabidiol for oral health: A new promising therapeutical tool in dentistry. Int. J. Mol. Sci. 2023;24(11):9693.
• Bonini SA, Premoli M, Tambaro S, Kumar A, Maccarinelli G, Memo M, Mastinu A. Cannabis sativa: A comprehensive ethnopharmacological review of a medicinal plant with a long history. J. Ethnopharmacol. 2018;227:300-315.
• Carvalho C, Souza JLMC, Coutinho TCL. Effect of different application frequencies of CPP-ACP and fluoride dentifrice on demineralized enamel: A laboratory study. Am. J. Dent. 2014;27(4):215-219.
• Cruz Martinez C, Diaz Gómez M, Oh MS. Use of traditional herbal medicine as an alternative in dental treatment in Mexican dentistry. A review. Pharm. Biol. 2017;55(1):1992-1998.
• de Mello Vieira AE, Delbem ACB, Sassaki KT, Rodrigues E, Cury JA, Cunha RF. Fluoride dose response in pH-cycling models using bovine enamel. Caries Res. 2005;39(6):514-520.
• Du M, Tai B, Jiang H, Zhong J, Greenspan D, Clark A. Efficacy of dentifrice containing bioactive glass (NovaMin) on dentine hypersensitivity. J. Dent. Res. 2004;83(Special Issue A):1586.
• Guerrieri A, Gaucher C, Bonte E, Lasfargues J. Minimal intervention dentistry part 4. Detection and diagnosis of initial caries lesions. Br. Dent. J. 2012;213(11):551-557.
• Haghgoo R, Ahmadvand M, Moshaverinia S. Remineralizing Effect of Topical NovaMin and Nano-hydroxyapatite on caries-like Lesions in Primary teeth. J. Contemp. Dent. Pract. (JCDP). 2016;17(8):645-649.
• Hicks J, Garcia-Godoy F, Flaitz C. Biological factors in dental caries: role of saliva and dental plaque in the dynamic process of demineralization and remineralization (part 1). J. Clin. Pediatr. Dent. 2004;28(1):47-52.
• Janakiram C, Venkitachalam R, Fontelo P, Iafolla TJ, Dye BA. Effectiveness of herbal oral care products in reducing dental plaque & gingivitis–a systematic review and meta-analysis. BMC Complement. Med. Ther. 2020;20(1):1-12.
• Jawale KD, Kamat SB, Patil JA, Nanjannawar GS, Chopade RV. Grape seed extract. An innovation in remineralization. J. Conserv. Dent. (JCD). 2017;20(6):415.
• Jo SY, Chong HJ, Lee EH, Chang NY, Chae JM, Cho JH, Kim SC, Kang KH. Effects of various toothpastes on remineralization of white spot lesions. Korean J. Orthod. 2014;44(3):113-118.
• Kanth MR, Prakash AR, Sreenath G, Reddy VS, Huldah S. Efficacy of specific plant products on microorganisms causing dental caries. J. Clin.Diagn. Res. (JCDR). 2016;10(12):ZM01.
• Korkut B, Korkut D, Yanikoglu F, Tagtekin D. Clinical assessment of demineralization and remineralization surrounding orthodontic brackets with FluoreCam. Asian Pac. J. Trop. Biomed. 2017;7(4):373-377.
• Layer TM. Development of a fluoridated, daily-use toothpaste containing NovaMin technology for the treatment of dentin hypersensitivity. J. Clin. Dent. 2011;22(3):59-61.
• Nagarathana C, Sakunthala B, Naveena Preethi P. An update on current remineralizing agent. Oral Health Dent. Manag. (OHDM). 2015;14(4):183-187.
• Philip N. State of the art enamel remineralization systems: the next frontier in caries management. Caries Res. 2019;53(3):284-295.
• Poureslami H. The effects of plant extracts on dental plaque and caries. Contemporary approach to dental caries. 2012;20:395-402.
• Shaheen SS, Reddy P, Hemalatha SR, Doshi D, Kulkarni S, Kumar M. Antimicrobial efficacy of ten commercially available herbal dentifrices against specific oral microflora–In vitro study. J. Clin. Diagn. Res. (JCDR). 2015;9(4):ZC42.
• Soi S, Vinayak V, Singhal A, Roy S. Fluorides and their role in demineralization and remineralization. J. Dent. Sci. Oral Rehabil. 2013;14:19-21.
• Stahl V, Vasudevan K. Comparison of efficacy of cannabinoids versus commercial oral care products in reducing bacterial content from dental plaque. A preliminary observation. Cureus. 2020;12(1).
• Sudjalim TR, Woods MG, Manton DJ, Reynolds EC. Prevention of demineralization around orthodontic brackets in vitro. Am. J. Orthod. Dentofacial Orthop. 2007;131(6):705.
• Şivet EA, Gökçe ANP, Kargül B. Effect of different remineralization agents on artificial caries lesion: An in-vitro study. Clin. Exp. Health Sci. 2023;13(2):330-336.
• Truta E, Surdu S, Rosu CM, Asaftei M. Hemp-Biochemical diversity and multiple uses. J. Exp. Molec. Biol. 2009;10(2).
• Ullah R, Zafar MS, Shahani N. Potential fluoride toxicity from oral medicaments. A review. Iran. J. Basic Med. Sci. 2017;20(8):841.
• Vasudevan K, Stahl V. Cannabinoids infused mouthwash products are as effective as chlorhexidine on inhibition of total-culturable bacterial content in dental plaque samples. J. Cannabis Res. 2020;2(1):1-9.