The optimization of growth parameters in a anodic chamber of a microbial fuel cell

Abstract

The pollution is a result of drastically rising fossil fuel usage due to increased global population and industrialization. Today’s world needs to replace fossil fuels with new and renewable energy sources. Microbial Fuel Cells (MFCs) are devices that convert the chemical energy to direct electricity by microbial metabolic activity. Microbial growth was actualized at the anodic chamber of MFC and must be controlled carefully by microbial methods. The main aim of this study is optimizing the growth parameters of microorganisms in two-chambered MFC with optical density, dried and wet weight of microorganisms that were grown at the anode chamber of MFC. Moreover, the Chemical Oxygen Demand (COD) values of medium were determined at fed cycles of MFC. The total volume of MFC was 0.7 L and the connection was made with the salt bridge between anode and cathode compartments. The anode chamber was used for bacterial growth that was taken from Akkaya Dam slime, was fed with molasses medium at 5 days intervals. The OD values were determined with a spectrophotometer at 600 nm, COD values were determined with the standard method and wet-dry weight also determined as a function of the incubation period.

Keywords

• COD removal,growth parameters,microbial fuel cells (MFCs),microbial growth,qm value

References

1. [1]. Du Z., Li H., Gu T, “A state of the art review on microbial fuel cells: A promising technology for wastewater treatment and bioenergy” Biotechnology Advances, 25, 464–482, 2007.
2. [2]. Çatal, T., Li, K., Bermek, H., Liu, H., “Electricity production from twelve monosaccharides using microbial fuel cells”, Journal of Power Sources, 175, 196–200, 2008.
3. [3]. Franks A. E., Nevin K. P., “Microbial Fuel Cells, A Current Review”, Energies, 3, 899-919, 2010.
4. [4]. Singh D., Pratap D., Baranwal Y., Kumar B., Chaudhary R. K., “Microbial fuel cells: A green technology for power generation”, Annals of Biological Research, 1(3), 128-138, 2010.
5. [5]. Bakhshian S., Kariminia H.R., Roshandel R., “Bioelectricity generation enhancement in a dual chamber microbial fuel cell under cathodic enzyme catalyzed dye decolorization” Bioresource Technology, 102(12):6761-5, 2011.
6. [6]. Herrero-Hernandez, E., Smith,T.J., Akid, R., “Electricity generation from wastewaters with starch as carbon source using a mediatorless microbial fuel cell”, Biosensors and Bioelectronics, 39, 194–198, 2013.
7. [7]. Lin C., Wu C., Chiu Y., Tsai S., “Effects of different mediators on electricity generation and microbial structure of a toluene powered microbial fuel cell”, Fuel, 125 30–3,. 2014.
8. [8]. Gude V. G.,” Wastewater treatment in microbial fuel cells e an overview”, Journal of Cleaner Production, 122, 287-307, 2016.

9. [9]. Trapero J. R., Horcajada L., Linares J. J., Lobato J., “Is microbial fuel cell technology ready? An economic answer towards industrial commercialization” Applied Energy, 185, 698–707, 2017.
10. [10]. Park D. H., Zeikus J.G., “Electricity generation in microbial fule cells using neutral red as an elektronophore”, Applied and Environmental Technology, 66(4), 1292 – 1297, 2000.
11. [11]. Larrosa-Guerrero A., Scott K., Head I.M., Mateo F., Ginesta A., Godinez C., “Effect of temperature on the performance of microbial fuel cells”, Fuel, 89, 3985–3994, 2010.
12. [12]. Zhang Y., Min B., Huang L., Angelidaki I., “Electricity generation and microbial community response to substrate changes in microbial fuel cell” Bioresource Technology, 102, 1166–1173, 2011.
13. [13]. Min Li X., Cheng K. Y., Selvam A., Wong J. W.C., “Bioelectricity production from acidic food waste leachate using microbial fuel cells: Effect of microbial inocula”, Process Biochemistry, 48, 283–288. 2013.
14. [14]. Penteado E.D., Fernandez-Marchante C.M., Zaiat M., Canizares P., Gonzalez E.R., Rodrigo M.A., “Influence of sludge age on the performance of MFC treating winery wastewater”, Chemosphere, 151, 163 – 170, 2016.
15. [15]. Agostino V., Ahmet D., Sacco A., Margaria V., Armato C., Quaglio M., “Electrochemical analysis of microbial fule cells based on enriched biofilm communities from freshwater sediment”, Electrochimica Acta, 237, 133 – 143. 2017.
16. [16]. Sirianuntapiboon S., Phothilangka P., Ohnomo S., “Decolorization of molasses wastewater by a strain No.BP103 of acetogenic bacteria” Bioresource Technology, 92, 31 – 39, 2004.
17. [17]. Wang ., Li B., Zeng Q., Liu H., “Antioxidant and free radical scavenging activities of pigments extracted from molasses alcohol wastewater” Food Chemistry, 107, 1198-1204, 2008.
18. [18]. Chae K., Choi M., Lee J., Kim K., Kim I. S., “Effect of different substrates on the performance, bacterial diversity, and bacterial viability in microbial fuel cells” Bioresource Technology, 100, 3518–352,. 2009.
19. [19]. Wang S., Huang L., Gan L., Quan X., Li N., Chen G., Lu L., Xing D., Yang F., “Combined effects of enrichment procedure and non-fermentable or fermentable co-substrate on performance and bacterial community for pentachlorophenol degradation in microbial fuel cells” Bioresource Technology, 120, 120–12,. 2012.
20. [20]. Ahn, Y., Logan, B. E., “Effectiveness of domestic wastewater treatment using microbial fuel cells at ambient and mesophilic temperatures”, Bioresource Technology, 10, 469–475, 2010.
21. [21]. Zhang X., He W., Ren L., Stager J., Evans P. J., Logan B. E., “COD removal characteristics in air-cathode microbial fuel cells” Bioresource Technology, 176, 23–31, 2015.
22. [22]. Logan B. E., Hamelers B., Rozendal R., Schroder U., Keller J., Freguıa S., Aelterman P., Verstraete W., Rabaey K., “Microbial Fuel Cells: Methodology and Technology” Environmental Science & Technology, Vol. 40, no. 17, 5181 – 5192, 2006.
23. [23]. Behera M., Jana P. S., Ghangrekar M.M., “Performance evaluation of low cost microbial fuel cell fabricated using earthen pot with biotic and abiotic cathode” Bioresource Technology, 101, 1183–1189, 2010.
24. [24]. Futamata H., Bretschger O., Cheung A., Kan J., Owen R., Nealson K.H., “Adaptation of soil microbes during establishment of microbial fuel cell consortium fed with lactate” Journal of Bioscience and Bioengineering, Vol. 115 No. 1, 58-63, 2013.
25. [25]. Dönmez G., “Bioaccumulation of the reactive textile dyes by Candida tropicalis growing in molasses medium”, Enzyme and Microbiol. Technology, 30, 363 – 366, 2001.