Isolation and characterization of fluoride resistant bacteria from groundwaters in Dindigul, Tamilnadu, India

Year 2018, Volume: 1 Issue: 2, 69 - 74, 01.04.2018

K. Kirupa Sree Chellaiah Edward Raja , U. Ramesh

Abstract

The use of microbes to
remove fluoride from water and soil is an extent of applied research and
development. This is the first attempt have been made to examine physiochemical
characteristics and also isolate fluoride resistant bacteria from ground waters
in selected villages at Dindigul district, Tamil nadu, India. Based on high
fluoride resistance (200 mM), three bacterial isolates were selected for
further studies. The isolates authentically identified as genus Pseudomonas. Biochemical and 16S rRNA
sequencing analysis of the isolates revealed that they are closely related
(97%) to Pseudomonas sp. (98%) to Pseudomonas aeruginosa and (97%) to Pseudomonas sp. The 16S rRNA sequences
were submitted in the NCBI under accession numbers MF481852, MF481853, MG751413.
The fluoride resistant bacterial strains were resistant to antibiotics such as
amoxicillin, ampicillin, chloramphenicol, kanamycin and streptomycin. 

Keywords

Ground water, Fluoride resistant bacteria, Antibiotic resistance, Pseudomonas

References

• Kiyoshi OMINE (Dr. Eng, Professor)Department of Civil EngineeringNagasaki University1-14 Bunkyo-machi, Nagasaki 852-8521, JAPANE-mail: [e-mail] [phone]　FAX [phone]
• Dr. G. MARIMUTHUINSA Senior ScientistDepartment of Animal Behavior & PhysiologySchool of Biological SciencesMadurai Kamaraj University Madurai 625 021, India. Ph: [phone]E-mail:[e-mail]
• Dr. U. RameshAssistant ProfessorDept. of Molecular BiologySchool of Biological SciencesMadurai Kamaraj UniversityMadurai-21, Indiaemail:[e-mail]