Simplified method for derivatization of extractable glyphosate and aminomethylphosphonic acid and their determination by high performance liquid chromatography

Year 2018, Volume: 1 Issue: 2, 19 - 30, 01.04.2018

Jamilu Garba Abd Wahid Samsuri Radziah Othman Muhammad Saiful Ahmad-hamdani

Abstract

This work presents a simple
procedure for pre-column derivatization of glyphosate and aminomethylphosphonic
acid (AMPA) and their determination by high-performance liquid chromatography
(HPLC). Derivatization was achieved by mixing a solution of 0.02 M FMOC-Cl,
0.05 M borate buffer and glyphosate or AMPA, then shaken for 1 hour, later
washed with diethyl ether and ready for analysis. The quantification was
performed by HPLC with fluorescent (FLD) or diode array detector (DAD). The
result of the HPLC-FLD/DAD showed high linearity (R² ≥ 0.995) of
both compounds over eight point’s concentration range and their high recovery
from water compared to soil matrixes. 
The relative standard deviation (RSD) range from 0.1 to 30 % from the
aforementioned matrixes. The limit of detection of HPLC-FLD for glyphosate from
water, sandy and clay soil was 0.008 mg L^-1, 0.021 and 0.132 mg kg^-1
respectively while that of AMPA was 0.004 mg L^-1, 0.74 and 0.224 mg
kg^-1. Meanwhile, the limit of detection of HPLC-DAD for glyphosate from
water, sandy and clay soils was 0.024 mg L^-1, 0.731 and 0.122 mg kg^-1
respectively while that of AMPA for water sample was 0.076 mg L^-1.  This study was unable to determine lower
detection limit for AMPA from soil matrixes by HPLC-DAD thus suggested for more
repeated extraction for increasing quantification of the compound.

Keywords

Glyphosate, aminomethylphosphonic acid, Derivatization, Analytical method, Water

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