5-FU Drug Loading During the Production of Hydroxyapatite-Gelatin and Hydroxyapatite-Chitosan Biocomposites in the Simulated Body Fluid Medium and Drug Release Studies

Year 2018, , 587 - 605, 30.12.2018

Nalan Erdöl Aydın

https://doi.org/10.18185/erzifbed.413332

Cited By: 2

Abstract

Hydroxyapatite, (HAp,Ca₁₀(PO₄)₆(OH)₂) is a bioceramic applied in different biomedical areas. In
recent studies, it has been concluded that hydroxyapatite nanoparticles has
inhibition effect on different kind of tumors. Therefore, HAp or composite
materials including HAp have been preferred in controlled drug delivery studies
as a drug carrier. However, hard structure and highly fragile structure
of hydroxyapatite limited its usage in clinical applications. This mechanical
disadvantages can be overcomed by using a polymer to produce a
hydroxyapatite-polymer biocomposite.In this study, hydroxyapatite-gelatin
(HAp-GEL) and hydroxyapatite-chitosan (HAp-CTS) biocomposites were produced by
wet precipitation method at pH 7.4 and 37°C implementing glutaraldehyde (GA) as a
cross-linking agent in the SBF (Simulated Body Fluid) medium. Drug loading
process was performed during the production of biocomposites by wet
precipitation method. By running experiments with different amounts (2% and 5%)
of glutaraldehyde (GA) and different biocomposites, the effect of
glutaraldehyde and type of composite on drug loading efficiency and drug
release profiles were investigated. All experiments were performed with
5-Fluorouracil (5-FU) drug. Drug loading performance of 5-FU at the HAp-GEL and
Hap-CTS biocomposites were determined in deionized water, phosphate buffer
solution (PBS) and HCl solution. To determine of drug loading and drug release
performace of the samples, UV spectrophotometer was used. Fourier transform
infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron
microscopy (SEM), thermogravimetric analysis (TGA) and particle size analyses were performed to
characterize the produced biocomposites.

Keywords

Biocomposite, Hydroxyapatite, Drug Release, Drug Loading, Gelatin, Chitosan

Hidroksiapatit-Jelatin ve Hidroksiapatit-Kitosan Biyokompozitlerin Yapay Vücut Sıvısı Ortamında Üretimi Sırasında 5-FU İlacının Yüklenmesi ve İlaç Salım Çalışmaları

Abstract

Hidroksiapatit,
(HAp,Ca₁₀(PO₄)₆(OH)₂) farklı medikal alanlarda uygulaması olan bir
biyoseramiktir. Son yapılan çalışmalarda,
nanoboyutlu HAp parçacıklarının farklı tümör türleri üzerinde gelişimi önleyici
etki gösterdiği görülmüştür. Bu nedenle, HAp veya HAp içeren kompozit
malzemeler kontrollü ilaç salımı uygulamalarında ilaç taşıyıcı ortam olarak
tercih edilmiştir. Ancak, sert yapısı ve yüksek kırılganlığı klinik
uygulamalarda kullanımını sınırlamaktadır. Bu mekanik dezavantajlar, polimer
kullanılarak hidroksiapatit-polimer biyokompozit üretilmesiyle aşılabilmektedir.Bu
çalışmada, hidroksiapatit-jelatin
(HAp-GEL) ve hidroksiapatit-kitosan (HAp-CTS) biyokompozitler, pH 7.4 ve 37°C’
de yaş çöktürme yöntemiyle, çapraz bağlama ajanı olarak gluteraldehitin (GA)
kullanıldığı SBF (Yapay Vücut Sıvısı) ortamında üretilmiştir. İlaç yüklemi
işlemi, biyokompozitlerin yaş çöktürme yöntemi ile üretimi sırasında
gerçekleştirilmiştir. Farklı miktarlarda (%2 ve %5) gluteraldehit (GA) ve
farklı biyokompozitler ile yürütülen deneylerde gluteraldehitin ve biyokompozit
türünün ilaç yükleme verimi ve ilaç salım profili üzerine etkisi incelenmiştir.
Tüm deneyler 5-Fluorouracil (5-FU) ilacı ile gerçekleştirilmiştir. HAp-GEL ve
Hap-CTS biyokompozitlerde 5-FU ilacının yüklenme performansı deiyonize su,
fosfat tampon çözelti (PBS) ve HCl çözelti ortamında incelenmiştir. Numunelerin
5-FU ilaç yükleme ve ilaç salımı performansının belirlenmesinde UV
spektrofotometre kullanılmıştır. Üretilen biyokompozitleri karakterize etmek
için, Fourier dönüşümlü kızılötesi spektroskopi (FTIR), X-ışını kırınımı (XRD),
taramalı elektron mikroskobu (SEM), termogravimetrik analiz (TGA) ve partikül
boyut analizleri yapılmıştır.

Keywords

Hidroksiapatit, Biyokompozit, İlaç Salım, İlaç Yükleme, Jelatin, Kitosan

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