Effect of Tianeptine on Oxidative Renal Damage Induced by Ischemia Reperfusion in Rats

Abstract

Renal ischemia-reperfusion (I/R) damage can develop following major surgical conditions such as shock, trauma, and renal transplantation and causes acute renal failure. Kidney I/R damage is known to be a pathological process that begins with the touch free of oxygen and continues with the production of free oxygen radicals. We will investigate the effect of renal I/R injury on tianeptine, an atypical antidepressant drug with antioxidant properties. There was no information on the effect of tianeptine on I/R-induced kidney damage in the literature. The aim of the present study was to investigate the effect of tianeptine on oxidative kidney damage induced by I/R in rats. Albino wistar male rats were divided into groups as renal I/R (RIR) group, 25 mg/kg of thianeptine+renal I/R (TIR-25) group and sham operation (SG) group. The I/R procedure (one hour ischemia, three hours reperfusion) was performed with 25 mg/kg intraperitoneal thiopental sodium anesthesia. Oxidative damage was assessed by malondialdehyde (MDA) and total glutathione (TGSH) measurements in kidney tissue. Biochemical test results showed that the I/R process increased the amount of MDA in the kidney tissue and decreased the tGSH. Tianeptine significantly prevented the increase of MDA and decrease of tGSH in kidney tissue after I/R. Our experimental results suggest that tianeptine may be useful in preventing I/R-induced oxidative renal injury.

Keywords

• Tianeptine,Ischemia/Reperfusion,Kidney,Malondialdehyde,Total glutathione

Tianeptinin Sıçanlarda İskemi Reperfüzyonla Oluşturulan Oksidatif Böbrek Hasarına Etkisi

Abstract

Böbrek iskemi-reperfüzyon (İ/R) hasarı  şok, travma ve böbrek transplantasyonu gibi majör cerrahi durumları takiben gelişebilmektedir ve akut böbrek yetmezliğine neden olmaktadır. Böbrek İ/R hasarının,   dokunun oksijensiz  kalması ile başlayan ve  serbest oksijen radikallerinin üretimi ile devam eden patolojik bir süreç olduğu bilinmektedir. Çalışmamızda böbrek İ/R hasarına etkisini araştıracağımız tianeptin,  antioksidan özelliğe sahip atipik antidepresan bir ilaçtır. Literetürlerde tianeptinin, İ/R ile oluşturulan böbrek hasarına karşı etkisine ait bilgilere rastlanmadı. Çalışmamızın amacı tianeptinin, sıçanlarda İ/R ile oluşturulan oksidatif böbrek hasarına etkisini araştırmaktır. Albino wistar türü erkek sıçanlar renal iskemi-reperfüzyon (RİR), 25 mg/kg tianeptin+renal iskemi-reperfüzyon (TİR-25) ve şam operasyonu uygulanacak (SG) gruplara  ayrıldı. İ/R işlemi (bir saat iskemi, üç saat reperfüzyon), 25 mg/kg intraperitoneal tiopental sodyum anestezisi uygulanarak yapıldı. Oksidatif hasar böbrek dokusunda malondialdehid (MDA) ve total glutatyon (tGSH) ölçümü ile değerlendirildi. Biyokimyasal deney sonuçları, İ/R işleminin böbrek dokusunda MDA miktarının artmasına tGSH’nın ise azalmasına neden olduğunu göstermiştir. Tianeptinin, böbrek dokusunda MDA’nın İ/R ile artmasını ve tGSH’nın azalmasını anlamlı önlediği saptanmıştır. Deney sonuçlarımız, tianeptinin İ/R ile indüklenen oksidatif böbrek hasarını önlemede yararlı olabileceğini düşündürmektedir.

Keywords

• Malondialdehid,Tianeptin,İskemi/Reperfüzyon,Böbrek,Total glutatyon

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